Laser micro-dissection microscopy of single Cell-compartments from human placental tissue: A new approach to study trophoblast differentiation

Abstract Diffuse midline gliomas driven by lysine27-to-methionine mutations in histone 3 (H3-K27M DMGs) are among the most fatal brain tumors. Molecular studies including single cell RNA-sequencing (scRNA-seq) of pediatric and predominantly pontine H3-K27M DMGs have shown that the H3-K27M oncohistone keeps glioma cells locked in a stem-like oligodendrocyte precursor cell (OPC) state that is capable of self-renewal and tumor-initiation. However, a comprehensive dissection of the cellular architecture of H3-K27M DMGs across different midline regions and age groups is required to better understand the cell-intrinsic and contextual regulation of H3-K27M DMG cell identities. In particular, the more recently described group of adult H3-K27M DMGs remains understudied. Here, we have collected and characterized 45 H3-K27M mutant patient tumors, spanning pontine (n=26), thalamic (n=17), and spinal (n=2) locations. Median age at surgery was 12 (2-68) years, encompassing 21 early childhood (0-10 years), 12 adolescent (11-20 years), and 12 adult (≥ 21 years) tumors. The majority of samples were obtained pre-treatment (n=28), as opposed to post-treatment or at autopsy (n=17). We profiled all 45 tumors by single cell/single nucleus RNA-seq and selected tumors were further characterized by the single cell assay for transposase-accessible chromatin (scATAC-seq). Our integrated analyses highlight the predominance of transcriptionally and epigenetically defined OPC-like tumor cells as the main cell population of H3-K27M DMGs across all age groups and locations. We further identify distinct age- and location-specific OPC-like cell subpopulations. Comparison of pediatric and adult tumors further demonstrates a significant increase of mesenchymal cell states in adult H3-K27M DMGs, which we link to differences in glioma-associated immune cell compartments between age groups. Together, this study sheds light on the effects of age- and region-dependent microenvironments in shaping cellular identities in H3-K27M DMGs.

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Metabolic Changes in Human Placental Tissue in Diabetes Mellitus

Fetal Nutrition, Metabolism, and Immunology ◽

10.1007/978-1-4684-1191-1_17 ◽

1984 ◽

pp. 209-221

Author(s):

Yoram Z. Diamant ◽

Rita Kissilevitz

Keyword(s):

Diabetes Mellitus ◽

Placental Tissue ◽

Metabolic Changes ◽

Human Placental Tissue

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Seasonally Variant Gene Expression in Full-Term Human Placenta

10.1101/2020.01.27.20018671 ◽

2020 ◽

Author(s):

Danielle A. Clarkson-Townsend ◽

Elizabeth Kennedy ◽

Todd M. Everson ◽

Maya A. Deyssenroth ◽

Amber A. Burt ◽

...

Keyword(s):

Health Outcomes ◽

Placental Tissue ◽

Later Life ◽

Full Term ◽

Season Of Birth ◽

Rhythmic Expression ◽

Health And Development ◽

Human Placental Tissue ◽

Variant Genes ◽

Ribosomal Activity

ABSTRACTSeasonal exposures influence human health and development. The placenta, as a mediator of the maternal and fetal systems and a regulator of development, is an ideal tissue to understand the biological pathways underlying relationships between season of birth and later life health outcomes. Here, we conducted a transcriptome-wide association study of season of birth in full-term human placental tissue to evaluate whether the placenta may be influenced by seasonal cues. Of the analyzed transcripts, 583 displayed differential expression between summer and winter births (FDR q<0.05); among these, BHLHE40, MIR210HG, and HILPDA had increased expression among winter births (Bonferroni p<0.05). Enrichment analyses of the seasonally variant genes between summer and winter births indicated over-representation of transcription factors HIF1A, VDR, and CLOCK, among others, and of GO term pathways related to ribosomal activity and infection. Additionally, a cosinor analysis found rhythmic expression for approximately 11.9% of all 17,664 analyzed placental transcripts. These results suggest that the placenta responds to seasonal cues and add to the growing body of evidence that the placenta acts as a peripheral clock, which may provide a molecular explanation for the extensive associations between season of birth and health outcomes.

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The specific 3H-ouabain binding to Na+/K+-ATPase of the human placental tissue is inhibited by newborn plasma extracts

Steroids ◽

10.1016/0039-128x(88)90148-1 ◽

1988 ◽

Vol 52 (4) ◽

pp. 363-364

Author(s):

Anna Paci ◽

Silvana Balzan ◽

Franca Cocci ◽

Hans Peter Niedermeyer ◽

Aldo Clerico

Keyword(s):

Placental Tissue ◽

Ouabain Binding ◽

Human Placental Tissue

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A new approach reveals syncytia within the visceral musculature ofDrosophila melanogaster

Development ◽

10.1242/dev.128.13.2517 ◽

2001 ◽

Vol 128 (13) ◽

pp. 2517-2524 ◽

Cited By ~ 1

Author(s):

Robert Klapper ◽

Sandra Heuser ◽

Thomas Strasser ◽

Wilfried Janning

Keyword(s):

Drosophila Melanogaster ◽

Single Cell ◽

Cell Transplantation ◽

Reporter Gene ◽

Mononuclear Cells ◽

Single Cells ◽

New Approach ◽

Transplantation Technique ◽

Somatic Musculature ◽

Somatic Muscles

In order to reveal syncytia within the visceral musculature of Drosophila melanogaster, we have combined the GAL4/UAS system with the single-cell transplantation technique. After transplantation of single cells from UAS-GFP donor embryos into ubiquitously GAL4-expressing recipients, the expression of the reporter gene was exclusively activated in syncytia containing both donor- and recipient-derived nuclei. In the first trial, we tested the system in the larval somatic musculature, which is already known to consist of syncytia. By this means we could show that most of the larval somatic muscles are generated by clonally non-related cells. Moreover, using this approach we were able to detect syncytia within the visceral musculature – a tissue that has previously been described as consisting of mononuclear cells. Both the longitudinal visceral musculature of the midgut and the circular musculature of the hindgut consist of syncytia and persist through metamorphosis. This novel application of the transplantation technique might be a powerful tool to trace syncytia in any organism using the GAL4/UAS system.

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From naive iPS cells toward human placental tissue via trophectoderm

Placenta ◽

10.1016/j.placenta.2021.08.047 ◽

2021 ◽

Vol 114 ◽

pp. 151-152

Author(s):

Shingo Io ◽

Eiji Kondoh ◽

Yasuhiro Takashima ◽

Masaki Mandai

Keyword(s):

Placental Tissue ◽

Ips Cells ◽

Human Placental Tissue

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Influence of Fenoterol on Glucose Utilization by Human Placental Tissue in an in vitro Perfusion System

In vitro Perfusion of Human Placental Tissue - Contributions to Gynecology and Obstetrics ◽

10.1159/000410693 ◽

1985 ◽

pp. 168-169 ◽

Cited By ~ 1

Author(s):

H. Zrubek ◽

M. Grudzien ◽

H. Sawulicka-Oleszczuk ◽

J. Oleszczuk ◽

T. Panczyk ◽

...

Keyword(s):

Glucose Utilization ◽

Placental Tissue ◽

Perfusion System ◽

In Vitro Perfusion ◽

Human Placental Tissue

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Course of placental 11beta-hydroxysteroid dehydrogenase type 2 and 15-hydroxyprostaglandin dehydrogenase mRNA expression during human gestation

Acta Endocrinologica ◽

10.1530/eje.0.1450187 ◽

2001 ◽

pp. 187-192 ◽

Cited By ~ 52

Author(s):

E Schoof ◽

M Girstl ◽

W Frobenius ◽

M Kirschbaum ◽

R Repp ◽

...

Keyword(s):

Gene Expression ◽

Mrna Expression ◽

Placental Tissue ◽

Hydroxysteroid Dehydrogenase ◽

Normal Pregnancy ◽

Premature Babies ◽

Gene Level ◽

Human Placental Tissue ◽

Human Placental Cells

BACKGROUND: During human pregnancy, 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2) plays an important role in protecting the fetus from high maternal glucocorticoid concentrations by converting cortisol to inactive cortisone. Furthermore, 11beta-HSD2 is indirectly involved in the regulation of the prostaglandin inactivating enzyme 15-hydroxyprostaglandin dehydrogenase (PGDH), because cortisol reduces the gene expression and enzyme activity of PGDH in human placental cells. OBJECTIVE: To examine developmental changes in placental 11beta-HSD2 and PGDH gene expression during the 2nd and 3rd trimesters of human pregnancies. METHODS: In placental tissue taken from 20 healthy women with normal pregnancy and 20 placentas of 17 mothers giving birth to premature babies, 11beta-HSD2 and PGDH mRNA expression was determined using quantitative real-time PCR. RESULTS: Placental mRNA expression of 11beta-HSD2 and PGDH increased significantly with gestational age (r=0.55, P=0.0002 and r=0.42, P=0.007). In addition, there was a significant correlation between the two enzymes (r=0.58, P<0.0001). CONCLUSIONS: In the course of pregnancy there is an increase in 11beta-HSD2 and PGDH mRNA expression in human placental tissue. This adaptation of 11beta-HSD2 prevents increasing maternal cortisol concentrations from transplacental passage and is exerted at the gene level. 11beta-HSD2 up-regulation may also lead to an increase in PGDH mRNA concentrations that, until term, possibly delays myometrial contractions induced by prostaglandins.

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