The periodontal bacterium Porphyromonas gingivalis impairs spiral artery remodelling and induces acute atherosis in a rat model of periodontitis

Placenta ◽  
2016 ◽  
Vol 45 ◽  
pp. 89
Author(s):  
Mary Brown ◽  
Priscilla Phillips ◽  
Ann Progulske-Fox ◽  
Leticia Reyes
Keyword(s):  
Porphyromonas Gingivalis ◽  
Rat Model ◽  
Spiral Artery ◽  
Acute Atherosis

scholarly journals Role of Porphyromonas gingivalis Phosphoserine Phosphatase Enzyme SerB in Inflammation, Immune Response, and Induction of Alveolar Bone Resorption in Rats

2010 ◽  
Vol 78 (11) ◽  
pp. 4560-4569 ◽  
Author(s):  
Brian Bainbridge ◽  
Raj K. Verma ◽  
Christie Eastman ◽  
Bilal Yehia ◽  
Mercedes Rivera ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Epithelial Cells ◽  
Periodontal Disease ◽  
Porphyromonas Gingivalis ◽  
Rat Model ◽  
Alveolar Bone ◽  
Critical Role ◽  
Polymorphonuclear Neutrophil ◽  
Gingival Epithelial Cells ◽  
Phosphatase Enzyme

ABSTRACT Porphyromonas gingivalis secretes a serine phosphatase enzyme, SerB, upon contact with gingival epithelial cells in vitro. The SerB protein plays a critical role in internalization and survival of the organism in epithelial cells. SerB is also responsible for the inhibition of interleukin-8 (IL-8) secretion from gingival epithelial cells infected with P. gingivalis. This study examined the ability of a P. gingivalis SerB mutant to colonize the oral cavity and induce gingival inflammation, immune responses, and alveolar bone resorption in a rat model of periodontal disease. Both P. gingivalis ATCC 33277 and an isogenic ΔSerB mutant colonized the oral cavities of rats during the 12-week experimental period. Both of the strains induced significant (P < 0.05) systemic levels of immunoglobulin G (IgG) and isotypes IgG1, IgG2a, and IgG2b, indicating the involvement of both T helper type 1 (Th1) and Th2 responses to infection. Both strains induced significantly (P < 0.05) higher levels of alveolar bone resorption in infected rats than in sham-infected control rats. However, horizontal and interproximal alveolar bone resorption induced by the SerB mutant was significantly (P < 0.05) lower than that induced by the parental strain. Rats infected with the ΔSerB mutant exhibited significantly higher levels of apical migration of the junctional epithelium (P < 0.01) and polymorphonuclear neutrophil (PMN) recruitment (P < 0.001) into the gingival tissues than rats infected with the wild type. In conclusion, in a rat model of periodontal disease, the SerB phosphatase of P. gingivalis is required for maximal alveolar bone resorption, and in the absence of SerB, more PMNs are recruited into the gingival tissues.

scholarly journals CD56 expression of intravascular trophoblasts defines a class of vasculopathy in preeclampsia and other pregnancy complications

2018 ◽  
Author(s):  
Peilin Zhang
Keyword(s):  
Gestational Diabetes ◽  
Fetal Distress ◽  
Spiral Artery ◽  
Phenotypic Switch ◽  
Trophoblastic Cell ◽  
Medial Necrosis ◽  
Cd56 Expression ◽  
Implantation Sites ◽  
Cellular Components ◽  
Acute Atherosis

AbstractWe have discovered the expression of CD56 on the intravascular trophoblasts in the maternal spiral artery at implantation sites, and we have also found the similar phenotypic switch of intravascular trophoblasts in the decidual vasculopathy in preeclampsia. Currently we have examined 124 placentas from the patients with preeclampsia and 84 placentas from patients with other pregnancy associated complications without preeclampsia. CD56 expression on the intravascular trophoblasts can be seen in classic decidual vasculopathy such as fibrinoid medial necrosis and acute atherosis. In addition, partial involvement of the decidual vessels with classic vasculopathy can also be identified by CD56 expression. The cellular components of the classic vasculopathy in preeclampsia showed immunoreactivity to cytokeratin and CD68, in addition to CD56 expression, indicating the fetal trophoblastic cell origin. The classic decidual vasculopathy including acute atherosis and fibrinoid medial necrosis can be unified and designated as CD56-related vasculopathy. The CD56 related vasculopathy is associated not only to preeclampsia but also to other pregnancy related complications, such as gestational diabetes, placental infarcts, intervillous thrombosis and other fetal distress syndromes. Our study defined a spectrum of decidual vasculopathy spanning from the classic preeclampsia to other complications important to pregnancy that can be highlighted by CD56 expression, pointing to different direction of pathogenesis of preeclampsia and other pregnancy associated complications.

Changes in endovascular trophoblast invasion and spiral artery remodelling at term in a transgenic preeclamptic rat model

Placenta ◽  
2010 ◽  
Vol 31 (4) ◽  
pp. 320-326 ◽  
Author(s):  
N. Geusens ◽  
L. Hering ◽  
S. Verlohren ◽  
C. Luyten ◽  
K. Drijkoningen ◽  
...  
Keyword(s):  
Rat Model ◽  
Trophoblast Invasion ◽  
Spiral Artery ◽  
Endovascular Trophoblast

Endovascular Trophoblast Invasion, Spiral Artery Remodelling and Uteroplacental Haemodynamics in a Transgenic Rat Model of Pre-eclampsia

Placenta ◽  
2008 ◽  
Vol 29 (7) ◽  
pp. 614-623 ◽  
Author(s):  
N. Geusens ◽  
S. Verlohren ◽  
C. Luyten ◽  
M. Taube ◽  
L. Hering ◽  
...  
Keyword(s):  
Rat Model ◽  
Trophoblast Invasion ◽  
Transgenic Rat ◽  
Spiral Artery ◽  
Endovascular Trophoblast ◽  
Transgenic Rat Model

scholarly journals Effect of Porphyromonas gingivalis infection on leukocyte count in rat model of diabetes mellitus

2018 ◽  
Vol 4 (1) ◽  
pp. 27
Author(s):  
Safira Niza Ulfita ◽  
Agustin Wulan Suci Dharmayanti ◽  
Budi Yuwono
Keyword(s):  
Diabetes Mellitus ◽  
Porphyromonas Gingivalis ◽  
Rat Model ◽  
Metabolic Disorder ◽  
Leukocyte Count ◽  
Systemic Disease ◽  
Cell Counting ◽  
The Third ◽  
Cellular Changes ◽  
Counting Chamber

Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia and become the third cause of death in Indonesia. The most common oral complications occured in DM is periodontitis. Porphyromonas gingivalis (Pg) is the main etiology of periodontitis, one of periodonthopathogen relate to systemic disease. However this theory still controversial, DM and periodontitis have the same pathogenesis, thus are cellular changes. Pg is predicted affect the total leukocytes count in DM. The aim of this study was to determine the effect of Pg infection to total leukocytes count in DM rat model. The rat model of DM was obtained by injection of streptozotocin with different dosage for 5 days sequentially then injected by Pg on distobuccal and distopalatal sulcus of the maxillary first molars for 19 days with interval 3 days. The total leukocytes were calculated manually by Neubauer improved cell counting chamber. The results demonstrated that rat model of DM which injected by Pg has the lowest total leukocytes count. Therefore, Pg infection decreased the total leukocytesin DM rat model.

Sign in / Sign up

Export Citation Format

Share Document