Nitrogen and phosphorus interaction and cytokinin: Responses of the primary root of Arabidopsis thaliana and the pdr1 mutant

Plant Science ◽  
2013 ◽  
Vol 198 ◽  
pp. 91-97 ◽  
Author(s):  
Taiguer Cerutti ◽  
Carla Andréa Delatorre
Keyword(s):  
Arabidopsis Thaliana ◽  
Primary Root ◽  
Nitrogen And Phosphorus

scholarly journals Two Expansin Genes, AtEXPA4 and AtEXPB5, Are Redundantly Required for Pollen Tube Growth and AtEXPA4 Is Involved in Primary Root Elongation in Arabidopsis thaliana

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 249
Author(s):  
Weimiao Liu ◽  
Liai Xu ◽  
Hui Lin ◽  
Jiashu Cao
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Cell Walls ◽  
Root Elongation ◽  
Expression Patterns ◽  
Primary Root ◽  
Pollen Grains ◽  
Tube Growth ◽  
Cell Wall Binding

The growth of plant cells is inseparable from relaxation and expansion of cell walls. Expansins are a class of cell wall binding proteins, which play important roles in the relaxation of cell walls. Although there are many members in expansin gene family, the functions of most expansin genes in plant growth and development are still poorly understood. In this study, the functions of two expansin genes, AtEXPA4 and AtEXPB5 were characterized in Arabidopsis thaliana. AtEXPA4 and AtEXPB5 displayed consistent expression patterns in mature pollen grains and pollen tubes, but AtEXPA4 also showed a high expression level in primary roots. Two single mutants, atexpa4 and atexpb5, showed normal reproductive development, whereas atexpa4atexpb5 double mutant was defective in pollen tube growth. Moreover, AtEXPA4 overexpression enhanced primary root elongation, on the contrary, knocking out AtEXPA4 made the growth of primary root slower. Our results indicated that AtEXPA4 and AtEXPB5 were redundantly involved in pollen tube growth and AtEXPA4 was required for primary root elongation.

Apical meristem exhaustion during determinate primary root growth in the moots koom 1 mutant of Arabidopsis thaliana

Planta ◽  
2011 ◽  
Vol 234 (6) ◽  
pp. 1163-1177 ◽  
Author(s):  
Alejandra Hernández-Barrera ◽  
Yamel Ugartechea-Chirino ◽  
Svetlana Shishkova ◽  
Selene Napsucialy-Mendivil ◽  
Aleš Soukup ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Root Growth ◽  
Apical Meristem ◽  
Primary Root ◽  
Primary Root Growth

scholarly journals Overexpression of a Novel Component Induces HAK5 and Enhances Growth in Arabidopsis

ISRN Botany ◽  
2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Eri Adams ◽  
Celine Diaz ◽  
Minami Matsui ◽  
Ryoung Shin
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Adaptive Response ◽  
Screening Method ◽  
Primary Root ◽  
Nutrient Deficiency ◽  
Potassium Deficiency ◽  
Low Potassium ◽  
Primary Root Growth ◽  
Gene Hunting

Plants have developed mechanisms to adapt to the potassium deficient conditions over the years. In Arabidopsis thaliana, expression of a potassium transporter HAK5 is induced in low potassium conditions as an adaptive response to nutrient deficiency. In order to understand the mechanism in which HAK5 is regulated, the full-length cDNA overexpressor gene hunting system was employed as a screening method. Of 40 genes recovered, At4g18280 was found to be dramatically induced in response to potassium-deficiency and salt stress. Plants overexpressing this gene showed higher HAK5 expression and enhanced growth. These plants were also less sensitive to potassium-deficiency in terms of primary root growth. Taken together, these data suggest that this novel component, At4g18280, contributes to regulation of HAK5 and, consequently, tolerance to potassium-deficiency in plants.

scholarly journals Exogenous Nitro-Oleic Acid Treatment Inhibits Primary Root Growth by Reducing the Mitosis in the Meristem in Arabidopsis thaliana

2020 ◽  
Vol 11 ◽  
Author(s):  
Luciano M. Di Fino ◽  
Ignacio Cerrudo ◽  
Sonia R. Salvatore ◽  
Francisco J. Schopfer ◽  
Carlos García-Mata ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Oleic Acid ◽  
Root Growth ◽  
Acid Treatment ◽  
Primary Root ◽  
Primary Root Growth

scholarly journals Nitrate Induction of Primary Root Growth Requires Cytokinin Signaling in Arabidopsis thaliana

2019 ◽  
Vol 61 (2) ◽  
pp. 342-352 ◽  
Author(s):  
Pamela A Naulin ◽  
Grace I Armijo ◽  
Andrea S Vega ◽  
Karem P Tamayo ◽  
Diana E Gras ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Division ◽  
Root Growth ◽  
Primary Root ◽  
Growth Arrest ◽  
Root Tip ◽  
Wild Type ◽  
Cytokinin Signaling ◽  
Control Growth ◽  
Primary Root Growth

Abstract Nitrate can act as a potent signal to control growth and development in plants. In this study, we show that nitrate is able to stimulate primary root growth via increased meristem activity and cytokinin signaling. Cytokinin perception and biosynthesis mutants displayed shorter roots as compared with wild-type plants when grown with nitrate as the only nitrogen source. Histological analysis of the root tip revealed decreased cell division and elongation in the cytokinin receptor double mutant ahk2/ahk4 as compared with wild-type plants under a sufficient nitrate regime. Interestingly, a nitrate-dependent root growth arrest was observed between days 5 and 6 after sowing. Wild-type plants were able to recover from this growth arrest, while cytokinin signaling or biosynthesis mutants were not. Transcriptome analysis revealed significant changes in gene expression after, but not before, this transition in contrasting genotypes and nitrate regimes. We identified genes involved in both cell division and elongation as potentially important for primary root growth in response to nitrate. Our results provide evidence linking nitrate and cytokinin signaling for the control of primary root growth in Arabidopsis thaliana.

Genetic and contig map of a 2200-kb region encompassing 5.5 cM on chromosome 1 of Arabidopsis thaliana

Genome ◽  
1996 ◽  
Vol 39 (6) ◽  
pp. 1086-1092 ◽  
Author(s):  
Christian S. Hardtke ◽  
Thomas Berleth
Keyword(s):  
Arabidopsis Thaliana ◽  
Positional Cloning ◽  
Yeast Artificial Chromosome ◽  
Physical Map ◽  
Primary Root ◽  
Artificial Chromosome ◽  
Chromosome 1 ◽  
Rflp Markers ◽  
Caps Markers ◽  
Yac Contig

In the course of the isolation of the MONOPTEROS (MP) gene, required for primary root formation in Arabidopsis thaliana, a yeast artificial chromosome (YAC) contig encompassing approximately 2200 kilobases corresponding to 5.5 cM on the top arm of chromosome 1 was established. Forty-six YAC clones were characterized and 12 new restriction fragment length polymorphism (RFLP) markers are presented. Three new codominant amplified polymorphic sequence (CAPS) markers were generated that enabled high resolution genetic mapping and correlation of physical and genetic distances along the contig. The map contributes to the completion of a physical map of the Arabidopsis genome and should facilitate positional cloning of other genes in the region as well as studies on genome organization. We also present another set of 11 physically linked probes, as well as mapping data for additional RFLP markers within a broader interval of 10.4 cM. Key words : Arabidopsis, CAPS markers, MONOPTEROS gene, physical map, RFLP markers, YAC contig.

scholarly journals Bradyrhizobium japonicum IRAT FA3 alters Arabidopsis thaliana root architecture via regulation of auxin efflux transporters PIN2, PIN3, PIN7 and ABCB19

2021 ◽  
Author(s):  
Mercedes Schroeder ◽  
Melissa Y. Gomez ◽  
Nathan K. McLain ◽  
Emma Gachomo
Keyword(s):  
Arabidopsis Thaliana ◽  
Root System ◽  
Root Development ◽  
Bradyrhizobium Japonicum ◽  
Lateral Root ◽  
Plant Root ◽  
Primary Root ◽  
Efflux Transporters ◽  
Loss Of Function ◽  
Lateral Root Primordia

Beneficial rhizobacteria can stimulate changes in plant root development. While root system growth is mediated by multiple factors, the regulated distribution of the phytohormone auxin within root tissues plays a principal role. Auxin transport facilitators help to generate the auxin gradients and maxima that determine root structure. Here, we show that the plant growth-promoting rhizobacterial strain Bradyrhizobium japonicum IRAT FA3 influences specific auxin efflux transporters to alter Arabidopsis thaliana root morphology. Gene expression profiling of host transcripts in control and B. japonicum-inoculated roots of the wild type A. thaliana accession Col-0 confirmed upregulation of PIN2, PIN3, PIN7 and ABCB19 with B. japonicum and identified genes potentially contributing to a diverse array of auxin-related responses. Co-cultivation of the bacterium with loss-of-function auxin efflux transport mutants revealed that B. japonicum requires PIN3, PIN7 and ABCB19 to increase lateral root development and utilizes PIN2 to reduce primary root length. Accelerated lateral root primordia production due to B. japonicum was not observed in single pin3, pin7 or abcb19 mutants, suggesting independent roles for PIN3, PIN7 and ABCB19 during the plant-microbe interaction. Our work demonstrates B. japonicum’s influence over host transcriptional reprogramming during plant interaction with this beneficial microbe and the subsequent alterations to root system architecture.

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