Concerted transcription of auxin and carbohydrate homeostasis-related genes underlies improved adventitious rooting of microcuttings derived from far-red treated Eucalyptus globulus Labill mother plants

2015 ◽  
Vol 97 ◽  
pp. 11-19 ◽  
Author(s):  
Carolina Michels Ruedell ◽  
Márcia Rodrigues de Almeida ◽  
Arthur Germano Fett-Neto
2019 ◽  
Vol 17 (3) ◽  
pp. e0803 ◽  
Author(s):  
Nikoleta-Kleio Denaxa ◽  
Peter A. Roussos ◽  
Stavros N. Vemmos ◽  
Konstantinos Fasseas

Aim of study: To assess the role of polyphenol oxidase (PPO), peroxidase (POD) and indole-3-acetic acid oxidase (IAAox) during adventitious rooting (Ar) in semi-hardwood cuttings of the easy-to-root olive cv. ‘Arbequina’ and the difficult-to-root cv. ‘Kalamata’. Simultaneously, a histological study was carried out in both cultivars to investigate the tissue related with Ar development.Area of study: The rooting experiments were carried out in ‘Kostelenos’ nurseries (Troizinia, Greece) and in Agricultural University of Athens.Material and methods: Plant material to set up the experiment was collected from current year shoots from 15-year-old mother plants of ‘Arbequina’ and ‘Kalamata’ at three different seasons (summer, autumn and spring). The auxin indole-3-butyric acid (IBA) at 2000 mg L-1 was used as rooting inducer.Main results: Analysis revealed that ‘Kalamata’ had significantly higher enzymatic activities before experiment onset and during Ar compared to ‘Arbequina’. Control cuttings of both cultivars exhibited increased enzymatic activities compared to IBA treated ones. IAAox was on average three times higher in ‘Kalamata’ than in ‘Arbequina’ and exhibited significant peaks during Ar. Similar peaks of POD and PPO activities were also detected. Histological analyses in ‘Kalamata’ revealed a continuous sheath of sclerenchyma ring and increased cortex thickness. Significant cell proliferation occurred in the phloem region in ‘Arbequina’ 15 days after planting and afterwards the root initials started developing in the secondary phloem from cambial cells.Research highlights: The differences in enzymatic activities as well as in stem anatomy could partly justify the different rooting ability of both cultivars.


Revista CERES ◽  
2016 ◽  
Vol 63 (5) ◽  
pp. 691-697 ◽  
Author(s):  
Rodrigo Sobreira Alexandre ◽  
Poliana Rangel Costa ◽  
Kristhiano Chagas ◽  
Livia Giro Mayrinck ◽  
João Antônio Dutra Giles ◽  
...  

ABSTRACT Passiflora mucronata Lam. is resistant to Fusarim oxysporum f. passifloraceae and therefore can be used as rootstock for the species Passiflora edulis Sims. f. flavicarpa. The rootstocks in this case can be vegetatively propagated through cuttings. The objective of this study was to evaluate the effect of different types of cuttings and different concentrations of indole-3-butyric acid (IBA) on the adventitious rooting of P. mucronata. The experiments were arranged in a completely randomized design with four replications of 16 cuttings each. In Experiment 1, the treatments consisted of the different types of cuttings from mother plants grown in protected environment: shoot tips; two leaves and two nodes; one leaf and one node; leafless with two nodes; leafless with one node. In Experiment 2, the cuttings were taken from field plants and treated with the following IBA concentrations: 0; 1000 mg kg-1 (0.0036456 mol L-1); 1500 mg kg-1 (0.0054684 mol L-1) and 2000 mg kg-1 (0.0072912 mol L-1). The characteristics evaluated in the experiments 1 and 2 were: survival (1 and 2); budding (1 and 2); shoot number (2), shoot length (2), number of shoot leaves (2);shoot dry mass (2); callusing (1); rooting (1 and 2); root number (1 and 2), largest root length (1 and 2), root volume (1 and 2), and root dry mass (1 and 2). We found that, instead the shoot tips, the cuttings of the type leafless with two nodes are the best for P. mucronata rooting. Neither the cuttings from greenhouse plants nor the cuttings from field plants require growth regulators for rooting of P. mucronata cuttings of the type leafless with two nodes.


2017 ◽  
Vol 8 ◽  
Author(s):  
Márcio L. Aumond ◽  
Artur T. de Araujo ◽  
Camila F. de Oliveira Junkes ◽  
Márcia R. de Almeida ◽  
Hélio N. Matsuura ◽  
...  

New Forests ◽  
2008 ◽  
Vol 36 (3) ◽  
pp. 261-271 ◽  
Author(s):  
Joséli Schwambach ◽  
Carolina Michels Ruedell ◽  
Márcia Rodrigues de Almeida ◽  
Ricardo Miguel Penchel ◽  
Elias Frank de Araújo ◽  
...  

2011 ◽  
Vol 5 (S7) ◽  
Author(s):  
Arthur Fett-Neto ◽  
Marcia De Almeida ◽  
Carolina Ruedell

2005 ◽  
Vol 25 (4) ◽  
pp. 487-494 ◽  
Author(s):  
J. Schwambach ◽  
C. Fadanelli ◽  
A. G. Fett-Neto

2020 ◽  
Vol 38 (3) ◽  
pp. 429-435
Author(s):  
Renata de Almeida Maggioni ◽  
Leandro Porto Latoh ◽  
Leandro Marcolino Vieira ◽  
Emilio Romanini Netto ◽  
Katia Christina Zuffellato-Ribas

The objective of this study was to evaluate the viability of the Ginkgo biloba mini-cutting technique, as well as the influence of substrates and different concentrations of indole butyric acid (IBA) on adventitious rooting in addition to the protein and sugar content in the mini-cutting. Mini-cuttings were 4 ± 1 cm in length, with the bases immersed in solutions of 0, 1000, 2000, and 3000 mg L-1 IBA. They were then planted in polypropylene tubes using two substrates (vermiculite and Tropstrato®) and maintained under greenhouse conditions for 60 d. The experiment was carried out with a 2 × 4 factorial scheme (substrates × IBA). There was no influence of IBA application on the promotion of rhizogenesis in Ginkgo biloba mini-cuttings. The rooting percentages were higher than 55% regardless of the treatment used. The vermiculite substrate showed a higher number of roots (4.94) and lower mortality (11.60) of mini-cuttings than Tropstrato®. We conclude that the mini-cutting technique is feasible for Ginkgo biloba, and the use of IBA is not necessary. We found that the induction ofadventitious rooting depended on the biochemical composition of the mother plants, due to the translocation of non-reducing sugars and leaf proteins for root formation.


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