Stomata regulation by tissue-specific expression of the Citrus sinensis MYB61 transcription factor improves water-use efficiency in Arabidopsis

2018 ◽  
Vol 130 ◽  
pp. 54-60 ◽  
Author(s):  
Jesús L. Romero-Romero ◽  
Claudio Inostroza-Blancheteau ◽  
Daniela Orellana ◽  
Felipe Aquea ◽  
Marjorie Reyes-Díaz ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Water Use Efficiency ◽  
Water Use ◽  
Citrus Sinensis ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Use Efficiency

scholarly journals Human acyl-CoA:cholesterol acyltransferase 2 gene expression in intestinal Caco-2 cells and in hepatocellular carcinoma

2006 ◽  
Vol 394 (3) ◽  
pp. 617-626 ◽  
Author(s):  
Bao-Liang Song ◽  
Can-Hua Wang ◽  
Xiao-Min Yao ◽  
Li Yang ◽  
Wen-Jing Zhang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Transcription Factor ◽  
Molecular Mechanism ◽  
Luciferase Reporter ◽  
Hepatoma Cell Line ◽  
Cis Elements ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Cdx2 Expression

Humans express two ACAT (acyl-CoA:cholesterol acyltransferase) genes, ACAT1 and ACAT2. ACAT1 is ubiquitously expressed, whereas ACAT2 is primarily expressed in intestinal mucosa and plays an important role in intestinal cholesterol absorption. To investigate the molecular mechanism(s) responsible for the tissue-specific expression of ACAT2, we identified five cis-elements within the human ACAT2 promoter, four for the intestinal-specific transcription factor CDX2 (caudal type homeobox transcription factor 2), and one for the transcription factor HNF1α (hepatocyte nuclear factor 1α). Results of luciferase reporter and electrophoretic mobility shift assays show that CDX2 and HNF1α exert a synergistic effect, enhancing the ACAT2 promoter activity through binding to these cis-elements. In undifferentiated Caco-2 cells, the ACAT2 expression is increased when exogenous CDX2 and/or HNF1α are expressed by co-transfection. In differentiated Caco-2 cells, the ACAT2 expression significantly decreases when the endogenous CDX2 or HNF1α expression is suppressed by using RNAi (RNA interference) technology. The expression levels of CDX2, HNF1α, and ACAT2 are all greatly increased when the Caco-2 cells differentiate to become intestinal-like cells. These results provide a molecular mechanism for the tissue-specific expression of ACAT2 in intestine. In normal adult human liver, CDX2 expression is not detectable and the ACAT2 expression is very low. In the hepatoma cell line HepG2 the CDX2 expression is elevated, accounting for its elevated ACAT2 expression. A high percentage (seven of fourteen) of liver samples from patients affected with hepatocellular carcinoma exhibited elevated ACAT2 expression. Thus, the elevated ACAT2 expression may serve as a new biomarker for certain form(s) of hepatocellular carcinoma.

scholarly journals The Arabidopsis GTL1 Transcription Factor Regulates Water Use Efficiency and Drought Tolerance by Modulating Stomatal Density via Transrepression of SDD1

2010 ◽  
Vol 22 (12) ◽  
pp. 4128-4141 ◽  
Author(s):  
Chan Yul Yoo ◽  
Heather E. Pence ◽  
Jing Bo Jin ◽  
Kenji Miura ◽  
Michael J. Gosney ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Water Use Efficiency ◽  
Water Use ◽  
Stomatal Density ◽  
Use Efficiency

scholarly journals A Genetic Pathway Composed of EDT1/HDG11, ERECTA, and E2Fa Loci Regulates Water Use Efficiency by Modulating Stomatal Density

2017 ◽  
Author(s):  
Xiao-Yu Guo ◽  
Yao Wang ◽  
Ping Xu ◽  
Guo-Hua Yu ◽  
Li-Yong Zhang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Water Use Efficiency ◽  
Cell Size ◽  
Water Use ◽  
Nuclear Dna ◽  
Stomatal Density ◽  
Nuclear Dna Content ◽  
Genetic Pathway ◽  
Use Efficiency

AbstractImprovement of crop drought resistance and water use efficiency (WUE) has been a major endeavor in agriculture. ERECTA is the first identified major effector of water use efficiency. However, the underlying molecular mechanism is not well understood. Here, we report a genetic pathway, composed of EDT1/HDG11, ERECTA, and E2Fa loci, which regulates water use efficiency by modulating stomatal density. The HD-START transcription factor EDT1/HDG11 transcriptionally activates ERECTA expression by binding to an HD cis-element in the ERECTA promoter. ERECTA in turn relies on E2Fa to control the expression of cell-cycle related genes and the transition from mitosis to endocycle, which leads to increased nuclear DNA content in leaf cells, and therefore increased cell size and decreased stomatal density. The decreased stomatal density improves plant WUE. Our study demonstrates the EDT1/HDG11-ERECTA-E2Fa genetic pathway that reduces stomatal density by increasing cell size, providing a new avenue to improve WUE of crops.

scholarly journals Poplar GTL1 Is a Ca2+/Calmodulin-Binding Transcription Factor that Functions in Plant Water Use Efficiency and Drought Tolerance

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e32925 ◽  
Author(s):  
Hua Weng ◽  
Chan Yul Yoo ◽  
Michael J. Gosney ◽  
Paul M. Hasegawa ◽  
Michael V. Mickelbart
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Water Use Efficiency ◽  
Water Use ◽  
Plant Water ◽  
Calmodulin Binding ◽  
Plant Water Use ◽  
Use Efficiency

scholarly journals Activity and tissue-specific expression of the transcription factor NF-E1 multigene family.

1990 ◽  
Vol 4 (10) ◽  
pp. 1650-1662 ◽  
Author(s):  
M Yamamoto ◽  
L J Ko ◽  
M W Leonard ◽  
H Beug ◽  
S H Orkin ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Multigene Family ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Future-Proofing Potato for Drought and Heat Tolerance by Overexpression of Hexokinase and SP6A

2021 ◽  
Vol 11 ◽  
Author(s):  
Günter G. Lehretz ◽  
Sophia Sonnewald ◽  
Nitsan Lugassi ◽  
David Granot ◽  
Uwe Sonnewald
Keyword(s):  
Heat Stress ◽  
Abiotic Stress ◽  
Water Use Efficiency ◽  
Water Use ◽  
Abiotic Stress Tolerance ◽  
Crop Productivity ◽  
Specific Expression ◽  
Potato Plants ◽  
Use Efficiency ◽  
Drought And Heat Stress

Crop yield is largely affected by global climate change. Especially periods of heat and drought limit crop productivity worldwide. According to current models of future climate scenarios, heatwaves and periods of drought are likely to increase. Potato, as an important food crop of temperate latitudes, is very sensitive to heat and drought which impact tuber yield and quality. To improve abiotic stress resilience of potato plants, we aimed at co-expressing hexokinase 1 from Arabidopsis thaliana (AtHXK1) in guard cells and SELF-PRUNING 6A (SP6A) using the leaf/stem-specific StLS1 promoter in order to increase water use efficiency as well as tuberization under drought and heat stress. Guard cell-specific expression of AtHXK1 decreased stomatal conductance and improved water use efficiency of transgenic potato plants as has been shown for other crop plants. Additionally, co-expression with the FT-homolog SP6A stimulated tuberization and improved assimilate allocation to developing tubers under control as well as under single and combined drought and heat stress conditions. Thus, co-expression of both proteins provides a novel strategy to improve abiotic stress tolerance of potato plants.

scholarly journals Epigenetics and expression of key genes associated with cardiac fibrosis: NLRP3, MMP2, MMP9, CCN2/CTGF, and AGT

2020 ◽  
Author(s):  
Sruti Chandra ◽  
Kenneth C. Ehrlich ◽  
Michelle Lacey ◽  
Carl Baribault ◽  
Melanie Ehrlich
Keyword(s):  
Extracellular Matrix ◽  
Transcription Factor ◽  
Cardiac Fibrosis ◽  
Transcription Factor Binding ◽  
Open Chromatin ◽  
Specific Expression ◽  
Tissue Specific ◽  
Factor Binding ◽  
Tissue Specific Expression ◽  
Super Enhancer

AbstractExcessive inflammatory signaling and pathological remodeling of the extracellular matrix are important contributors to cardiac fibrosis and involve major changes in gene expression. We examined the relationships between tissue-specific expression and the epigenetics of five genes involved in these pathways, NLRP3, MMP2, MMP9, CCN2/CTGF, and AGT. The proteins encoded by these genes play major fibrosis-related roles in inflammasome formation, degradation of extracellular matrix proteins and remodeling of the extracellular matrix and vasculature, autocrine regulation of fibrosis, or cell signaling. Our analyses showed that the first four of these genes had super-enhancers (unusually strong enhancer clusters) that correlate with their very high expression in monocytes, neutrophils, fibroblasts, or venous cells. Expression of the gene encoding miR-223, a micro-RNA that plays an important role in downregulating NLRP3 protein levels, is also probably driven by the super-enhancer in which it is embedded. Enhancer chromatin for all these genes was inside as well as outside the gene body. While AGT, which encodes precursors of angiotensin II, lacked a super-enhancer, its tissue-specific expression profile correlates with the tissue-specific enhancer chromatin extending into its distant silent gene neighbor (CAPN9). Tissue-specific peaks of DNA hypomethylation, open chromatin (DNaseI hypersensitivity), and transcription factor binding were detected in subregions of these super-enhancers/enhancers that are likely to be the main drivers of expression of their associated gene. We found that CCN2/CTGF is co-expressed with its far-upstream neighbor LINC01013, a noncoding RNA gene, specifically in vein endothelial cells (HUVEC) and chondrocytes. Evidence from chromatin looping profiling (Hi-C) suggests coregulation of these genes in HUVEC. Our findings indicate the importance of understanding the often-overlooked roles of enhancers and their hypomethylated, transcription factor-binding subregions in the regulation of expression of fibrosis-related genes in normal and fibrotic tissue.

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