Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran

2010 ◽  
Vol 45 (1) ◽  
pp. 120-128 ◽  
Author(s):  
Khushal Brijwani ◽  
Harinder Singh Oberoi ◽  
Praveen V. Vadlani
Keyword(s):  
Solid State ◽  
Mixed Culture ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme System ◽  
Cellulolytic Enzyme ◽  
Soybean Hulls

Optimization of Solid State Fermentation of Mixed-Culture of Penicillium Consortium for Cellulase and Lytic Polysaccharide Monooxygenases Production

2021 ◽  
Vol 15 (2) ◽  
pp. 180-187
Author(s):  
Zi-Han Xu ◽  
Cui-Yi Liang ◽  
Wen Wang ◽  
Qiong Wang ◽  
Wei Qi ◽  
...  
Keyword(s):  
Solid State ◽  
Mixed Culture ◽  
Solid State Fermentation ◽  
Process Condition ◽  
Nitrogen Sources ◽  
Cellulolytic Enzyme ◽  
Mixture Ratio ◽  
Lytic Polysaccharide Monooxygenases ◽  
Polysaccharide Monooxygenases ◽  
Ssf Process

In order to produce higher titre of cellulolytic enzyme cocktails, present study deals with four Penicillium consortium mixed-culture and to investigate their feasibility of producing higher cellulase and auxiliary enzymes via solid state fermentation (SSF). Among different lignocellulosic waste, rice straw was found to be the most suitable substrate. In addition, the Penicillium consortium in a mixture ratio of 1:1:1:1 which exhibited higher enzyme activity than the monoculture. Nitrogen sources of tryptone had significant influences on cellulase and lytic polysaccharide monooxygenases (LPMOs) production. The highest cellulase and LPMO activities were 16.65 ± 1.83 U/g and 33.87 ± 0.45 U/g, respectively. The SSF process condition used to obtain these activities were at 30 °C during 5 days. These results show that the optimized studied of mixed-cultivation system with Penicillium consortium have potential to be exploiting a complex consortium for the enrichment of cellulolytic enzyme cocktails for bioethanol processes.

Upgrading of valuable food component contents and anti-nutritional factors depletion by solid-state fermentation: A way to valorize wheat bran for nutrition

2020 ◽  
pp. 103159
Author(s):  
Sonja Jakovetić Tanasković ◽  
Nataša Šekuljica ◽  
Jelena Jovanović ◽  
Ivana Gazikalović ◽  
Sanja Grbavčić ◽  
...  
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Food Component ◽  
Nutritional Factors

scholarly journals Comparative Evaluation of Agroindustrial Byproducts for the Production of Alkaline Protease by Wild and Mutant Strains ofBacillus subtilisin Submerged and Solid State Fermentation

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Hamid Mukhtar ◽  
Ikramul Haq
Keyword(s):  
Bacillus Subtilis ◽  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Alkaline Protease ◽  
Enzyme Production ◽  
Enhanced Production ◽  
Industrial Byproducts ◽  
Agroindustrial Byproducts

The present study describes the screening of different agroindustrial byproducts for enhanced production of alkaline protease by a wild and EMS induced mutant strain ofBacillus subtilisIH-72EMS8. During submerged fermentation, different agro-industrial byproducts were tested which include defatted seed meals of rape, guar, sunflower, gluten, cotton, soybean, and gram. In addition to these meals, rice bran, wheat bran, and wheat flour were also evaluated for protease production. Of all the byproducts tested, soybean meal at a concentration of 20 g/L gave maximum production of the enzyme, that is, 5.74  ±  0.26 U/mL from wild and 11.28  ±  0.45 U/mL from mutant strain, during submerged fermentation. Different mesh sizes (coarse, medium, and fine) of the soybean meal were also evaluated, and a finely ground soybean meal (fine mesh) was found to be the best. In addition to the defatted seed meals, their alkali extracts were also tested for the production of alkaline protease byBacillus subtilis, but these were proved nonsignificant for enhanced production of the enzyme. The production of the enzyme was also studied in solid state fermentation, and different agro-industrial byproducts were also evaluated for enzyme production. Wheat bran partially replaced with guar meal was found as the best substrate for maximum enzyme production under solid state fermentation conditions.

scholarly journals Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct

2004 ◽  
Vol 47 (5) ◽  
pp. 813-819 ◽  
Author(s):  
Natalia Martin ◽  
Simone Regina de Souza ◽  
Roberto da Silva ◽  
Eleni Gomes
Keyword(s):  
Solid State ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Sugar Cane Bagasse ◽  
Pectin Lyase ◽  
Optimum Activity ◽  
Fungal Strains ◽  
Penicillium Sp ◽  
Polygalacturonase Production ◽  
Pectinase Production

Pectin lyase and polygalacturonase production by newly isolated fungal strains was carried out in solid-state fermentation. Moniliella SB9 and Penicillium sp EGC5 produced polygalcturonase (PG) and pectin lyase (PL) on mixture of orange bagasse, sugar cane bagasse and wheat bran as substrate. PG and PL produced by Moniliella presented optimum activity at pH 4.5 and 10.0 and at 55 and 45°C, respectively, while these enzymes from Penicillium sp presented optimum activity at pH 4.5-5.0 and 9.0 and 40°C, respectively.

Integrated Strategies to Enhance Cellulolytic Enzyme Production Using an Instrumented Bioreactor for Solid-State Fermentation of Sugarcane Bagasse

2012 ◽  
Vol 6 (1) ◽  
pp. 142-152 ◽  
Author(s):  
Ursula Fabiola Rodríguez-Zúñiga ◽  
Sonia Couri ◽  
Victor Bertucci Neto ◽  
Silvio Crestana ◽  
Cristiane Sanchez Farinas
Keyword(s):  
Solid State ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Cellulolytic Enzyme ◽  
Integrated Strategies
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