Purification of pyranose oxidase from the white rot fungus Irpex lacteus and its cooperation with laccase in lignin degradation

2014 ◽  
Vol 49 (12) ◽  
pp. 2191-2198 ◽  
Author(s):  
Ming-Qiang Ai ◽  
Fang-Fang Wang ◽  
Yu-Zhong Zhang ◽  
Feng Huang
Keyword(s):  
Lignin Degradation ◽  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Pyranose Oxidase

scholarly journals Alkali treatment of fungal pretreated wheat straw for bioethanol production

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
María García-Torreiro ◽  
Miguel Álvarez Pallín ◽  
María López-Abelairas ◽  
Thelmo A. Lu-Chau ◽  
Juan M. Lema
Keyword(s):  
Wheat Straw ◽  
Alkali Treatment ◽  
Alkaline Treatment ◽  
White Rot ◽  
White Rot Fungus ◽  
Process Conditions ◽  
Alkali Pretreatment ◽  
Irpex Lacteus ◽  
C 30 ◽  
Pretreated Wheat Straw

AbstractBioconversion of lignocellulosic materials into ethanol requires an intermediate pretreatment step for conditioning biomass. Sugar yields from wheat straw were previously improved by the addition of a mild alkali pretreatment step before bioconversion by the white-rot fungus Irpex lacteus. In this work, an alternative alkaline treatment, which significantly reduces water consumption, was implemented and optimized. Sugar recovery increased 117% with respect to the previously developed alkaline wash process at optimal process conditions (30°C, 30 minutes and 35.7% (w/w) of NaOH). In order to further reduce operational costs, a system for alkali recycling was implemented. This resulted in the treatment of 150% more wheat straw using the same amount of NaOH. Finally, enzymatic hydrolysis was optimized and resulted in a reduction of enzyme dose of 33%.

Irpex lacteus, a white-rot fungus with biotechnological potential — review

2009 ◽  
Vol 54 (5) ◽  
pp. 375-390 ◽  
Author(s):  
Č. Novotný ◽  
T. Cajthaml ◽  
K. Svobodová ◽  
M. Šušla ◽  
V. Šašek
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Biotechnological Potential

scholarly journals Characterization of Acetylxylan Esterase from White-Rot Fungus Irpex lacteus

2019 ◽  
Vol 66 (4) ◽  
pp. 131-137
Author(s):  
Sangho Koh ◽  
Seika Imamura ◽  
Naoto Fujino ◽  
Masahiro Mizuno ◽  
Nobuaki Sato ◽  
...  
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Acetylxylan Esterase

Bioremediation of the heavy metal complex dye Isolan Dark Blue 2SGL-01 by white rot fungus Irpex lacteus

2011 ◽  
Vol 198 ◽  
pp. 198-205 ◽  
Author(s):  
Duraisamy Kalpana ◽  
Jae Hong Shim ◽  
Byung-Taek Oh ◽  
Kalaiselvi Senthil ◽  
Yang Soo Lee
Keyword(s):  
Heavy Metal ◽  
Metal Complex ◽  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Dark Blue

Iron and calcium translocation from pure gypsum and iron-amended gypsum by two brown rot fungi and a white rot fungus

Holzforschung ◽  
2008 ◽  
Vol 62 (6) ◽  
Author(s):  
Jonathan S. Schilling ◽  
Kaitlyn M. Bissonnette
Keyword(s):  
Building Materials ◽  
Woody Debris ◽  
White Rot Fungi ◽  
Brown Rot ◽  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Brown Rot Fungi ◽  
Ca Oxalate ◽  
Iron And Calcium

AbstractWood-degrading fungi commonly grow in contact with calcium (Ca)-containing building materials and may import Ca and iron (Fe) from soil into forest woody debris. For brown rot fungi, imported Ca2+may neutralize oxalate, while Fe3+may facilitate Fenton-based degradation mechanisms. We previously demonstrated, in two independent trials, that degradation of spruce by wood-degrading fungi was not promoted when Ca or Fe were imported from gypsum or metallic Fe, respectively. Here, we tested pine wood with lower endogenous Ca than the spruce blocks used in prior experiments, and included a pure gypsum treatment and one amended with 1% with FeSO4. Electron microscopy with microanalysis verified that brown rot fungiSerpula himantioidesandGloeophyllum trabeumand the white rot fungusIrpex lacteusgrew on gypsum and produced iron-free Ca-oxalate crystals away from the gypsum surface. Wood cation analysis verified significant Fe import by both brown rot isolates in Fe-containing treatments. Wood degradation was highest in Fe-gypsum-containing treatments for all three fungi, although only wood degraded byI. lacteushad significant Ca import. We suggest that Fe impurities may not exacerbate brown rot, and that both brown and white rot fungi may utilize Ca-containing materials.

Enhancement of lignin degradation and laccase activity in Pleurotus ostreatus by cotton stalk extract

1998 ◽  
Vol 44 (7) ◽  
pp. 676-680 ◽  
Author(s):  
Orly Ardon ◽  
Zohar Kerem ◽  
Yitzhak Hadar
Keyword(s):  
Oxygen Consumption ◽  
Pleurotus Ostreatus ◽  
Lignin Degradation ◽  
Laccase Activity ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Biodegradation ◽  
Cotton Stalk ◽  
Fermentation System ◽  
Uv Visible

The white rot fungus Pleurotus ostreatus was grown in a chemically defined solid state fermentation system amended with cotton stalk extract (CSE).Treated cultures exhibited increased laccase activity as well as enhanced lignin mineralization. Mineralization of [14C]lignin initialized 4 days earlier in CSE-supplemented cultures than in control cultures. Total mineralization in the first 16 days was 15% in the CSE-treated cultures, compared with only 7% in the controls. Cotton stalk extract also contained compounds that serve as substrates for laccase purified from P. ostreatus as shown by oxygen consumption, as well as changes in the UV–visible spectrum.Key words: cotton, Pleurotusostreatus, white rot, laccase, lignin biodegradation.

Lignin degrading system of white-rot fungi and its exploitation for dye decolorization

2002 ◽  
Vol 48 (10) ◽  
pp. 857-870 ◽  
Author(s):  
Vishal Shah ◽  
Frantisek Nerud
Keyword(s):  
Lignin Degradation ◽  
White Rot Fungi ◽  
Alcohol Oxidase ◽  
Dye Decolorization ◽  
White Rot ◽  
Low Molecular Weight ◽  
Low Molecular Weight Compounds ◽  
Pyranose Oxidase ◽  
Degrading System ◽  
Glyoxal Oxidase

With global attention and research now focused on looking for the abatement of pollution, white-rot fungi is one of the hopes of the future. The lignin-degrading ability of these fungi have been the focus of attention for many years and have been exploited for a wide array of human benefits. This review highlights the various enzymes produced by white-rot fungi for lignin degradation, namely laccases, peroxidases, aryl alcohol oxidase, glyoxal oxidase, and pyranose oxidase. Also discussed are the various radicals and low molecular weight compounds that are being produced by white-rot fungi and its role in lignin degradation. A brief summary on the developments in research of decolorization of dyes using white-rot fungi has been made.Key words: lignin degradation, white-rot fungi, laccase, peroxidase, radicals, dye decolorization.

scholarly journals Factors Governing Degradation of Phenol in Pharmaceutical Wastewater by White-rot Fungi: A Batch Study

2015 ◽  
Vol 9 (1) ◽  
pp. 93-99 ◽  
Author(s):  
M. Bernats ◽  
T. Juhna
Keyword(s):  
Synthetic Medium ◽  
Biomass Concentration ◽  
White Rot Fungi ◽  
Total Phenol ◽  
White Rot ◽  
White Rot Fungus ◽  
Optimal Conditions ◽  
Pharmaceutical Wastewater ◽  
Irpex Lacteus ◽  
Low Efficiency

Phenol is a major contaminant in the industrial water effluent, including pharmaceutical wastewaters. Although several physic-chemical methods for removal of phenol exist, they are of high cost, low efficiency, and generate toxic by-products. Thus, there is a need to develop technologies for biological removal of phenol from wastewater. In this study, the degradation of phenol in pharmaceutical wastewater by monoculture of white-rot fungi was studied. The degradation rate of total phenol in batch flasks by four fungal monocultures of Trametes versicolor, Phanerochaete chrysosporium, Gloeophyllum trabeum and Irpex lacteus in synthetic medium was compared. The results showed that white-rot fungus T.Versicolor was the most effective of the species. Further selection tests of optimal conditions of biomass concentration, pH and temperature were done, indicating that optimal conditions of degradation are at pH 5-6, temperature 25 °C, and biomass inoculum 10% (v/v). Under optimal conditions, total phenol was reduced by 93%, concentration of total phenol decreasing from 420±12 mg/l to 29±1 mg/l in seven days, with T.Versicolor specie. This study suggested that biological treatment with fungi may effectively be used as a pre-treatment stage for removal of phenol before polishing wastewater with conventional biological methods.

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