Characterization of an exopolysaccharide produced by Lactobacillus plantarum HM47 isolated from human breast milk

2018 ◽  
Vol 73 ◽  
pp. 15-22 ◽  
Author(s):  
Reshma B Nambiar ◽  
Periyar Selvam Sellamuthu ◽  
Anand Babu Perumal ◽  
Emmanuel Rotimi Sadiku ◽  
Goitse Phiri ◽  
...  
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Human Breast Milk ◽  
Human Breast

Stress tolerance in Lactobacillus plantarum LMF6 isolated from human breast milk

2020 ◽  
Vol 9 (5) ◽  
pp. 173-184
Author(s):  
Fatima Zohra Baghdad Belhadj ◽  
Faiza Boublenza ◽  
Nour-Eddine Karam
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Food Industry ◽  
Stress Proteins ◽  
Acid Stress ◽  
Human Breast Milk ◽  
Human Breast ◽  
Beneficial Effects ◽  
Sds Page ◽  
Extreme Heat Stress

Lactobacillus plantarum is a lactic acid bacterium widely used in the food industry because of its beneficial effects on human health and its ability of adaptation to different stress conditions, hence the purpose of this work was to study the adaptation abilities of Lactobacillus plantarum LM6 and stress proteins involved during this adapta on. Lb. plantarum LMF6 was isolated from human breast milk and was exposed to acid, alkaline, thermal, oxidative, osmotic, detergent and nutritional stresses in order to determine their effects on growth, viability, tolerance and mortality. SDS-PAGE electrophoresis allowed us to compare the total proteins in the absence and in the presence of stress then the ImageJ® so ware analyzed the obtained pro les. The results show that Lb. plantarum LMF6 is highly tolerant to osmo c (at 9% NaCl, the UFC number is 3.4×1010 UFC/ml), alkaline (4.7×107UFC/ml at pH10), detergent (the UFC number is close to the control), oxydative (3.3×108 UFC/ml), nutri onnal (5.2×107 UFC/ml), acid (pH5, pH4 and pH3) and heat (40°C, 45°C and 50°C with 1.45×1011, 2.78×109 and 2.80×108UFC/ml respec vely) stresses, but sensi ve to extreme acid stress (pH1 and pH2 with mortality rate variable from 5log to 10log) and extreme heat stress (55°C and 60°C when mortality increases to 8log at 60°C). Comparison of proteins profiles allowed us to see quantitative and qualitative differences. Our results allowed to say that Lb. plantarum LMF6 showed interesting characteristics and could be used in food industry as probio c lactobacilli.

scholarly journals Evaluation of probiotic properties of Lactobacillus plantarum WLPL04 isolated from human breast milk

2016 ◽  
Vol 99 (3) ◽  
pp. 1736-1746 ◽  
Author(s):  
Meiling Jiang ◽  
Fen Zhang ◽  
Cuixiang Wan ◽  
Yonghua Xiong ◽  
Nagendra P. Shah ◽  
...  
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Human Breast Milk ◽  
Probiotic Properties ◽  
Human Breast

scholarly journals Draft Genome Sequence of Lactobacillus plantarum WLPL04, Isolated from Human Breast Milk

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
XueYing Tao ◽  
Meiling Jiang ◽  
Feng Zhang ◽  
Feng Xu ◽  
Hua Wei
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Genome Sequence ◽  
Draft Genome ◽  
Healthy Woman ◽  
Draft Genome Sequence ◽  
Trna Genes ◽  
Human Breast Milk ◽  
Human Breast ◽  
Protein Coding

Lactobacillus plantarum WLPL04, a novel strain, was isolated from a breast milk sample from a healthy woman and demonstrated several probiotic functions. Here, we present the draft genome sequence of this strain, which contains 3,192,587 bp, a G+C content of 44.52%, 3,158 protein-coding genes, and 53 tRNA genes.

scholarly journals Characterization of the Cultivable Microbiota in Fresh and Stored Mature Human Breast Milk

2019 ◽  
Vol 10 ◽  
Author(s):  
Clarissa Schwab ◽  
Evelyn Voney ◽  
Alejandro Ramirez Garcia ◽  
Michaela Vischer ◽  
Christophe Lacroix
Keyword(s):  
Breast Milk ◽  
Human Breast Milk ◽  
Human Breast

scholarly journals Characterization of Biosurfactant Produced by Probiotic Bacteria Isolated from Human Breast Milk

2018 ◽  
Vol 3 (1) ◽  
pp. 18-24
Author(s):  
Laya Tabrizi Rayeni ◽  
Shahla Soltani Nezhad
Keyword(s):  
Breast Milk ◽  
Gene Sequencing ◽  
Human Breast Milk ◽  
Sequencing Analysis ◽  
Human Breast ◽  
16S Rdna Gene ◽  
Pediococcus Pentosaceus ◽  
Milk Samples ◽  
16S Rdna Gene Sequencing

Background: Breast milk is an important nutrient source for rapidly growing neonates since breastfeeding protects the newborn against some disease. This effect may be due to the useful and natural microflora of breast milk. Biosurfactants are unique amphipathic compounds produced by some microorganisms. The present study demonstrates the isolation and characterization of biosurfactant producing bacteria from human breast milk samples. Methods: The human breast milk samples were collected aseptically and then cultured in MRS agar media. The biosurfactant producing ability of the isolated strains was investigated by hemolytic assay, oil spreading method, drop collapse test and emulsification index assay. The screened isolates were identified by 16S rDNA gene sequencing analysis. In vitro antibacterial activities of biosurfactants against some Gram-positive and Gram negative bacteria were investigated by the agar disc diffusion method. This biosurfactant was characterized with Fourier transform infrared spectroscopy (FTIR). Results: In this study, 337 different colonies were isolated from 42 breast milk samples. The best isolates were identified as Pediococcus pentosaceus HM-1, Pediococcus pentosaceus HM-2 and Pediococcus pentosaceus HM-3 based on microscopic and 16S rDNA gene sequencing analysis. The biosurfactant extracted from screened strains exhibited a broad spectrum of antagonistic activity against some pathogenic bacteria. The results showed similarity to lipopeptide biosurfactants like surfactin. Conclusion: Bacterial strains isolated in this study could be valuable sources for novel biosurfactants. The Human breast milk could be a safe source for isolation of biosurfactant producing probiotic bacteria and for improve intestinal microflora of infants.

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