Stress tolerance in Lactobacillus plantarum LMF6 isolated from human breast milk

2020 ◽  
Vol 9 (5) ◽  
pp. 173-184
Author(s):  
Fatima Zohra Baghdad Belhadj ◽  
Faiza Boublenza ◽  
Nour-Eddine Karam
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Food Industry ◽  
Stress Proteins ◽  
Acid Stress ◽  
Human Breast Milk ◽  
Human Breast ◽  
Beneficial Effects ◽  
Sds Page ◽  
Extreme Heat Stress

Lactobacillus plantarum is a lactic acid bacterium widely used in the food industry because of its beneficial effects on human health and its ability of adaptation to different stress conditions, hence the purpose of this work was to study the adaptation abilities of Lactobacillus plantarum LM6 and stress proteins involved during this adapta on. Lb. plantarum LMF6 was isolated from human breast milk and was exposed to acid, alkaline, thermal, oxidative, osmotic, detergent and nutritional stresses in order to determine their effects on growth, viability, tolerance and mortality. SDS-PAGE electrophoresis allowed us to compare the total proteins in the absence and in the presence of stress then the ImageJ® so ware analyzed the obtained pro les. The results show that Lb. plantarum LMF6 is highly tolerant to osmo c (at 9% NaCl, the UFC number is 3.4×1010 UFC/ml), alkaline (4.7×107UFC/ml at pH10), detergent (the UFC number is close to the control), oxydative (3.3×108 UFC/ml), nutri onnal (5.2×107 UFC/ml), acid (pH5, pH4 and pH3) and heat (40°C, 45°C and 50°C with 1.45×1011, 2.78×109 and 2.80×108UFC/ml respec vely) stresses, but sensi ve to extreme acid stress (pH1 and pH2 with mortality rate variable from 5log to 10log) and extreme heat stress (55°C and 60°C when mortality increases to 8log at 60°C). Comparison of proteins profiles allowed us to see quantitative and qualitative differences. Our results allowed to say that Lb. plantarum LMF6 showed interesting characteristics and could be used in food industry as probio c lactobacilli.

scholarly journals Evaluation of probiotic properties of Lactobacillus plantarum WLPL04 isolated from human breast milk

2016 ◽  
Vol 99 (3) ◽  
pp. 1736-1746 ◽  
Author(s):  
Meiling Jiang ◽  
Fen Zhang ◽  
Cuixiang Wan ◽  
Yonghua Xiong ◽  
Nagendra P. Shah ◽  
...  
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Human Breast Milk ◽  
Probiotic Properties ◽  
Human Breast

scholarly journals Draft Genome Sequence of Lactobacillus plantarum WLPL04, Isolated from Human Breast Milk

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
XueYing Tao ◽  
Meiling Jiang ◽  
Feng Zhang ◽  
Feng Xu ◽  
Hua Wei
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Genome Sequence ◽  
Draft Genome ◽  
Healthy Woman ◽  
Draft Genome Sequence ◽  
Trna Genes ◽  
Human Breast Milk ◽  
Human Breast ◽  
Protein Coding

Lactobacillus plantarum WLPL04, a novel strain, was isolated from a breast milk sample from a healthy woman and demonstrated several probiotic functions. Here, we present the draft genome sequence of this strain, which contains 3,192,587 bp, a G+C content of 44.52%, 3,158 protein-coding genes, and 53 tRNA genes.

scholarly journals Perspective: The Role of Human Breast-Milk Extracellular Vesicles in Child Health and Disease

2020 ◽  
Author(s):  
Daniel O'Reilly ◽  
Denis Dorodnykh ◽  
Nina V Avdeenko ◽  
Nikita A Nekliudov ◽  
Johan Garssen ◽  
...  
Keyword(s):  
Breast Milk ◽  
Extracellular Vesicles ◽  
Basic Research ◽  
Normative Values ◽  
Future Research ◽  
Human Breast Milk ◽  
Human Breast ◽  
Mature Milk ◽  
Bioactive Substances ◽  
Beneficial Effects

ABSTRACT Human breast milk (HM) contains multiple bioactive substances determining its impact on children's health. Extracellular vesicles (EVs) are a heterogeneous group of secreted nanoparticles that are present in HM and may be partially responsible for its beneficial effects. The precise roles and content of EVs in HM remain largely unknown. To examine this, we performed a short narrative review on the literature focusing on HM EVs to contextualize the available data, followed by a scoping review of MEDLINE and Embase databases. We identified 424 nonduplicate citations with 19 original studies included. In this perspective, we summarize the evidence around HM EVs, highlight some theoretical considerations based on existing evidence, and provide an overview of some challenges associated with the complexity and heterogeneity of EV research. We consider how the existing data from HM studies conform to the minimal information for studies of EVs (MISEV) guidelines. Across the studies a variety of research methods were utilized involving both bench-based and translational methods, and a range of different EV contents were examined including RNA, proteins, and glycopeptides. We observed a variety of health outcomes in these studies, including allergy and atopy, necrotizing enterocolitis, and HIV. While some promising results have been demonstrated, the heterogeneity in outcomes of interest, methodological limitations, and relatively small number of studies in the field make comparison between studies or further translational work problematic. To date, no studies have examined normative values of HM EVs in a large, diverse population or with respect to potentially important influencing factors such as timing (hind- vs. foremilk), stage (colostrum vs. mature milk), and infant age (preterm vs. term), which makes extrapolation from bench or “basic” research impossible. Future research should focus on addressing the current inadequacies in the literature and utilize MISEV guidelines to inform study design.

Characterization of an exopolysaccharide produced by Lactobacillus plantarum HM47 isolated from human breast milk

2018 ◽  
Vol 73 ◽  
pp. 15-22 ◽  
Author(s):  
Reshma B Nambiar ◽  
Periyar Selvam Sellamuthu ◽  
Anand Babu Perumal ◽  
Emmanuel Rotimi Sadiku ◽  
Goitse Phiri ◽  
...  
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Human Breast Milk ◽  
Human Breast

Organohalogen compounds in human breast milk from Republic of Buryatia, Russia

2007 ◽  
Vol 146 (1) ◽  
pp. 225-232 ◽  
Author(s):  
Oyuna V. Tsydenova ◽  
Agus Sudaryanto ◽  
Natsuko Kajiwara ◽  
Tatsuya Kunisue ◽  
Valeriy B. Batoev ◽  
...  
Keyword(s):  
Breast Milk ◽  
Human Breast Milk ◽  
Human Breast ◽  
Organohalogen Compounds

Human breast milk suppresses the transcriptional regulation of IL-1β-induced NF-κB signaling in human intestinal cells

2004 ◽  
Vol 287 (5) ◽  
pp. C1404-C1411 ◽  
Author(s):  
Ryoko Minekawa ◽  
Takashi Takeda ◽  
Masahiro Sakata ◽  
Masami Hayashi ◽  
Aki Isobe ◽  
...  
Keyword(s):  
Breast Milk ◽  
Pathogenic Bacteria ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Nuclear Factor Κb ◽  
Protective Role ◽  
Intestinal Cells ◽  
Human Breast Milk ◽  
Human Breast ◽  
Activation Pathway

Neonatal necrotizing enterocolitis (NEC), which is a disease with a poor prognosis, is considered to be caused by the coincidence of intestinal ischemia-reperfusion injury and systemic inflammation due to the colonization of pathogenic bacteria. Interleukin (IL)-8, a proinflammatory cytokine, plays an important role in the pathophysiology of NEC. It was recently reported that IL-1β activates the IL-8 gene by regulating the transcriptional nuclear factor κB (NF-κB) signaling pathways in intestinal cells. The protective role of maternal milk in NEC pathogenesis has been reported in both human and animal studies. In this study, we show that human breast milk dramatically suppressed the IL-1β-induced activation of the IL-8 gene promoter by inhibiting the activation pathway of NF-κB. Moreover, we also show that human breast milk induced the production of IκBα. These results suggest that human breast milk could be protective and therapeutic in neonates with NEC by inhibiting the activation pathway of NF-κB.

scholarly journals Comparison of Vitamin D and 25-Hydroxyvitamin D Concentrations in Human Breast Milk between 1989 and 2016–2017

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 573
Author(s):  
Naoko Tsugawa ◽  
Mayu Nishino ◽  
Akiko Kuwabara ◽  
Honami Ogasawara ◽  
Maya Kamao ◽  
...  
Keyword(s):  
Vitamin D ◽  
Breast Milk ◽  
Sun Exposure ◽  
Stepwise Multiple Regression ◽  
Human Breast Milk ◽  
Vitamin D Status ◽  
Human Breast ◽  
Hydroxyvitamin D ◽  
Lactating Mothers ◽  
25 Hydroxyvitamin D

Background: Breast milk is considered the optimal source of nutrition during infancy. Although the vitamin D concentration in human breast milk is generally considered poor for infants, vitamin D in breast milk is an important source for exclusively breastfed infants. Increases in vitamin D insufficiency and deficiency in lactating mothers may reduce vitamin D concentrations in breast milk. This study aimed to compare vitamin D and 25-hydroxyvitamin D (25OHD) concentrations in breast milk collected in 1989 and 2016–2017 and simultaneously analyze them with liquid chromatography-tandem mass spectrometry (LC-MS/MS); the association between the lifestyle of recent lactating mothers (2016–2017) and vitamin D status in human breast milk was also evaluated. Method: Lactating mothers were recruited from three regions of Japan in 1989 (n = 72) and 2016–2017 (n = 90), and milk from 3–4 months was collected in summer and winter. The samples were strictly sealed and stored at −80℃ until measurement. Breast milk vitamin D and 25OHD concentrations were analyzed by LC-MS/MS. Vitamin D intake, sun exposure, and sunscreen use of the lactating mothers in 2016–2017 were assessed. Results: Both vitamin D and 25OHD concentrations in breast milk were higher in the summer regardless of the survey year. Significantly lower vitamin D and 25OHD concentrations were observed in 2016–2017 compared with 1989 in summer, but no survey year difference was observed in winter. The stepwise multiple regression analyses identified season, daily outdoor activity, and suntan in the last 12 months as independent factors associated with vitamin D3 concentrations. Conclusion: The results suggest that low vitamin D status in recent lactating mothers may have decreased vitamin D and 25OHD concentrations in breast milk compared with the 1980s. These results are helpful for developing public health strategies to improve vitamin D status in lactating mothers and infants.

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