Biocatalytic transesterification of algal oil employing a heterogenous methanol tolerant lipase enzyme aggregate from Bacillus mycoides strain CV18

2021 ◽  
Author(s):  
Disha Sharma ◽  
Shivani Uniyal ◽  
Lakshmi Tewari ◽  
Disha Sharma
Keyword(s):  
Bacillus Mycoides ◽  
Algal Oil ◽  
Lipase Enzyme ◽  
Methanol Tolerant

EFFECT OF LIPASE ENZYME ON THE RIPENING OF WHITE PICKLED CHEESE

2001 ◽  
Vol 8 (3) ◽  
pp. 205-213 ◽  
Author(s):  
SERDAR AYDEMIR ◽  
NIHAT AKIN ◽  
CELALETTIN KOÇAK
Keyword(s):  
Lipase Enzyme

scholarly journals Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Azhar Najjar ◽  
Elhagag Ahmed Hassan ◽  
Nidal Zabermawi ◽  
Saber H. Saber ◽  
Leena H. Bajrai ◽  
...  
Keyword(s):  
Ph Value ◽  
Energy Content ◽  
Value Added ◽  
Relative Activity ◽  
Economic Feasibility ◽  
Biodiesel Production ◽  
Molar Ratios ◽  
Cooking Oil ◽  
Enzyme Biocatalysis ◽  
Methanol Tolerant

AbstractIn this study, two highly thermotolerant and methanol-tolerant lipase-producing bacteria were isolated from cooking oil and they exhibited a high number of catalytic lipase activities recording 18.65 ± 0.68 U/mL and 13.14 ± 0.03 U/mL, respectively. Bacterial isolates were identified according to phenotypic and genotypic 16S rRNA characterization as Kocuria flava ASU5 (MT919305) and Bacillus circulans ASU11 (MT919306). Lipases produced from Kocuria flava ASU5 showed the highest methanol tolerance, recording 98.4% relative activity as well as exhibited high thermostability and alkaline stability. Under the optimum conditions obtained from 3D plots of response surface methodology design, the Kocuria flava ASU5 biocatalyst exhibited an 83.08% yield of biodiesel at optimized reaction variables of, 60 ○C, pH value 8 and 1:2 oil/alcohol molar ratios in the reaction mixture. As well as, the obtained results showed the interactions of temperature/methanol were significant effects, whereas this was not noted in the case of temperature/pH and pH/methanol interactions. The obtained amount of biodiesel from cooking oil was 83.08%, which was analyzed by a GC/Ms profile. The produced biodiesel was confirmed by Fourier-transform infrared spectroscopy (FTIR) approaches showing an absorption band at 1743 cm−1, which is recognized for its absorption in the carbonyl group (C=O) which is characteristic of ester absorption. The energy content generated from biodiesel synthesized was estimated as 12,628.5 kJ/mol. Consequently, Kocuria flava MT919305 may provide promising thermostable, methanol-tolerant lipases, which may improve the economic feasibility and biotechnology of enzyme biocatalysis in the synthesis of value-added green chemicals.

Evaluation of Bacillus mycoides isolate BmJ and B. mojavensis isolate 203-7 for the control of anthracnose of cucurbits caused by Glomerella cingulata var. orbiculare

2009 ◽  
Vol 48 (2) ◽  
pp. 140-146 ◽  
Author(s):  
Oliver T. Neher ◽  
Mareike R. Johnston ◽  
Nina K. Zidack ◽  
Barry J. Jacobsen
Keyword(s):  
Glomerella Cingulata ◽  
Bacillus Mycoides

Methanol-Tolerant Oxygen Reduction Reaction at Pt–Pd/C Alloy Nanocatalysts

2010 ◽  
Vol 8 (1) ◽  
Author(s):  
A. Mary Remona ◽  
K. L. N. Phani
Keyword(s):  
Oxygen Reduction Reaction ◽  
Oxygen Reduction ◽  
Rotating Disk ◽  
Reduction Reaction ◽  
Rotating Disk Electrode ◽  
Methanol Tolerance ◽  
Pd Catalysts ◽  
Onset Potential ◽  
Direct Methanol ◽  
Methanol Tolerant

Carbon-supported platinum and Pt–Pd alloy electrocatalysts with different Pt/Pd atomic ratios were synthesized by a microemulsion method at room temperature (metal loading is 10 wt %). The Pt–Pd/C bimetallic catalysts showed a single-phase fcc structure and the mean particle size of Pt–Pd/C catalysts was found to be lower than that of Pt/C. The methanol-tolerant studies of the catalysts were carried out by activity evaluation of oxygen reduction reaction (ORR) on Pt–Pd catalysts using a rotating disk electrode (RDE). The studies indicated that the order of methanol tolerance was found to be PtPd3/C>PtPd/C>Pt3Pd/C. The oxygen reduction activities of all Pt–Pd/C were considerably larger than that of Pt/C with respect to onset and overpotential values. The Pd-loaded catalysts shift the onset potential of ORR by 125 mVMSE, 53 mVMSE, and 41 mVMSE to less cathodic potentials for Pt3Pd/C, PtPd/C, and PtPd3/C, respectively, with reference to Pt/C and the Pt3Pd/C catalyst showed greater shift in the onset value than the other PtPd catalysts reported in literature. Moreover, the Pt–Pd/C catalysts exhibited much higher methanol tolerance during ORR than the Pt/C, assessing that these catalysts may function as a methanol-tolerant cathode catalysts in a direct methanol fuel cell.

Safety evaluation of DHA-rich Algal Oil from Schizochytrium sp.

2011 ◽  
Vol 49 (12) ◽  
pp. 3310-3318 ◽  
Author(s):  
I. Fedorova-Dahms ◽  
P.A. Marone ◽  
M. Bauter ◽  
A.S. Ryan
Keyword(s):  
Safety Evaluation ◽  
Algal Oil

Enhancing biosurfactant production from an indigenous strain of Bacillus mycoides by optimizing the growth conditions using a response surface methodology

2010 ◽  
Vol 163 (3) ◽  
pp. 188-194 ◽  
Author(s):  
A.R. Najafi ◽  
M.R. Rahimpour ◽  
A.H. Jahanmiri ◽  
R. Roostaazad ◽  
D. Arabian ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Growth Conditions ◽  
Biosurfactant Production ◽  
Bacillus Mycoides
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