Effect of Akt activation on apoptosis-related gene expression in the crop tissues of male and female pigeons (Columba livia)

Apoptosis or programmed cell death is involved in many diseases include end-stage renal failure. Apoptosis-related genes include both stimulate genes and inhibitory gene of apoptosis. The genes which stimulate apoptosis include p53 and p21-WAF. The genes which inhibit apoptosis include bcl-2 gene family. The mechanisms of apoptosis include p53-dependend pathway and p53- independent pathway. We hypothesized that apoptosis-related genes may activate in renal graft rejection after kidney transplantation. Therefore, the present study was designed to investigate apoptosis-related gene expression and localization by immunohistochemical staining (IHCS) in human renal tissues with graft rejection and compare with that in normal human renal tissue.Human renal biopsy (n=5) were obtained after kidney transplantation with mild and moderate renal rejection. Normal human kidney biopsy was obtained during nephrectomy. P53, p21-WAF and Bcl-2 levels in renal tissue were determined by IHCS. The results of IHCS was evaluated by IHCS staining density scores (0, no staining; 1, minimal staining; 2, mild staining; 3, moderate staining; and 4, strong staining).

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Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients

PLoS ONE ◽

10.1371/journal.pone.0165582 ◽

2016 ◽

Vol 11 (11) ◽

pp. e0165582 ◽

Cited By ~ 8

Author(s):

Saskia MC Langemeijer ◽

Niccolo Mariani ◽

Ruth Knops ◽

Christian Gilissen ◽

Rob Woestenenk ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Expression Profiling ◽

Hematopoietic Cell ◽

Related Gene ◽

Cell Fractions ◽

Apoptosis Related Gene

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Proliferation and Apoptosis-Related Gene Expression in Experimental Acquired Immunodeficiency Syndrome-Related Simian Lymphoma

Blood ◽

10.1182/blood.v93.4.1364.404k19_1364_1371 ◽

1999 ◽

Vol 93 (4) ◽

pp. 1364-1371

Author(s):

Esmeralda Castaños-Vélez ◽

Thomas Heiden ◽

Marianne Ekman ◽

Joseph Lawrence ◽

Gunnel Biberfeld ◽

...

Keyword(s):

Gene Expression ◽

Acquired Immunodeficiency Syndrome ◽

Proliferative Activity ◽

Epstein Barr Virus ◽

Related Gene ◽

Barr Virus ◽

Acquired Immunodeficiency ◽

Epstein Barr ◽

Immunodeficiency Syndrome ◽

Apoptosis Related Gene

Lymphomas in 10 cynomolgus monkeys infected with a simian immunodeficiency virus (SIVsm) were studied with regard to proliferative activity and apoptosis-related gene expression. All were diffuse large-cell lymphomas, showed mono or oligoclonality and a 9/10 diploid cellular DNA content. Expression of a simian homologue to Epstein-Barr virus (HVMF-1) was shown in nine cases. The lymphomas showed moderate to high proliferative activity by Ki67 immunostaining and DNA flow cytometry, and a low number of apoptotic cells detected by TdT-mediated dUTP nick-end labeling (TUNEL). Immunohistochemistry showed abundant tumor infiltrating TIA-1+ cytotoxic lymphocytes (CTL) and macrophages. Bcl-2, Mcl-1, and also Bax and Bak, but not p53 were demonstrable in the tumor cells by immunostaining. Our findings suggest a causal relationship between HVMF-1 infection and a low apoptotic index of the lymphomas due to the expression of Bcl-2. The apparent inefficient function of tumor-infiltrating CTL could be due to inactivation of CTL and/or resistance of the lymphoma cells to CTL effects. The tumors showed immunoreactivity for CD18, CD29, and CD49d, but not for CD11a, mimicking the phenotype of human Epstein-Barr virus (EBV)–related lymphomas. In summary, our observations indicate a high similarity between this simian model of acquired immunodeficiency syndrome (AIDS)-related lymphomas (ARL) and human ARL and other immunosuppression-related lymphomas.

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