Double ionophore application may improve rates of blastocyst formation and clinical pregnancy in cases of failed single application

2021 ◽  
Author(s):  
Omar Shebl ◽  
Elisabeth Reiter ◽  
Sabine Enengl ◽  
Christina Allerstorfer ◽  
Gudrun Schappacher-Tilp ◽  
...  
Keyword(s):  
Clinical Pregnancy ◽  
Blastocyst Formation ◽  
Single Application

scholarly journals Increasing dominant follicular proportion negatively associated with good clinical outcomes in GnRH-a prolonged protocol: a large-sample retrospective analysis

2021 ◽  
Author(s):  
Houming Su ◽  
Youhua Lai ◽  
Jie Li ◽  
Tingting Liao ◽  
Licheng Ji ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
Retrospective Analysis ◽  
Formation Rate ◽  
Clinical Pregnancy ◽  
Blastocyst Formation ◽  
Negatively Associated ◽  
Large Sample ◽  
Group A ◽  
Group B

Abstract Background: Nowadays, there is no universal criteria for trigger time during controlled ovarian hyperstimulation (COH). Particularly, in the so-called GnRH-a prolonged protocol, widely used in China, the ideal time to trigger ovulation is not yet well defined. Methods: This was a large-sample retrospective analysis. Between January 2016 and January 2020, 1,925 young patients who underwent their first in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles with normal ovarian response were divided into three groups based on their dominant follicular proportions (DFP, defined as ≥18 mm follicles /≥14 mm follicles; Group A: < 30%; Group B: 30% - 60%; Group C: ≥ 60%). Binary logistic regression and multivariate linear regression were used to assessed whether DFP levels were related to clinical pregnancy, number of blastocysts frozen, blastocyst formation rate and low blastocysts frozen. Results: Binary logistics regression analysis showed that compared with Group A, the OR for clinical pregnancy was 1.345 in Group B (P = 0.023); however, there was no statistical difference between Group C and Group A (P = 0.216). On one hand, multivariate linear regression analysis indicated that DFP was negatively associated with number of blastocysts frozen (β ± SE: Group B vs Group A = -0.319 ± 0.115, P = 0.006; Group C vs Group A = -0.432 ± 0.154, P = 0.005) as well as blastocyst formation rate (β ± SE: Group B vs Group A = -0.035 ± 0.016, P = 0.031; Group C vs Group A = -0.039 ± 0.021, P = 0.067). On the other hand, compared with group A, the OR for low blastocyst frozen was 1.312 in Group B (P = 0.039) and was 1.417 in Group C (P = 0.041). Conclusions: Excessive delay of trigger in GnRH-a prolonged protocol might reduce the developmental potential of oocytes and reduce the number of available blastocysts, which might result in a lower cumulative pregnancy rate. But further confirmation by strict prospective randomized controlled study should be needed.Trial registration: https://clinicaltrials.gov/; NCT03305510; Registered 08 October 2017 - Retrospectively registered.

scholarly journals Laser-assisted hatching in lower grade cleavage stage embryos improves blastocyst formation: results from a retrospective study

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Weihai Xu ◽  
Ling Zhang ◽  
Lin Zhang ◽  
Zhen Jin ◽  
Limei Wu ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Subsequent Development ◽  
Clinical Pregnancy ◽  
Clinical Pregnancy Rate ◽  
Assisted Hatching ◽  
Lower Grade ◽  
Blastocyst Formation ◽  
Cleavage Stage ◽  
Good Grade ◽  
Blastocyst Rate

Abstract Background Laser-assisted hatching (LAH) has been widely applied to facilitate blastocyst hatching in IVF-ET treatment, however, the effect of LAH on subsequent development and clinical outcomes of the lower grade cleavage stage embryos (LGCE) remains unknown. Our study aimed at evaluating the effect of LAH on blastocyst formation and the clinical pregnancy outcomes of LGCE embryos after transfer. Methods A total of 608 cycles of IVF/ICSI treatment from November 2017 to September 2019 were included in our study as follows: 296 in the LAH group and 312 in the N-LAH group. The total blastocyst rate, usable blastocyst rate, good-grade blastocyst rate and clinical pregnancy rate were statistically compared between the two groups. Results The total blastocyst rate (50.7% vs 40.2%, P < 0.001), usable blastocyst rate (31.0% vs 18.6%, P < 0.001) were significantly higher in the LAH group than those in the N-LAH group. After analysis of generalized estimating equations, LAH was positively correlated with the blastocyst rate (B = 0.201, OR 95% CI = 1.074–1.393, P = 0.002), usable blastocyst rate (B = 0.478, OR 95% CI = 1.331–1.955, P < 0.001). However, the clinical pregnancy rate after blastocyst transfer did not differ between LAH group and N-LAH group (49.4% vs 40.0%, P > 0.05, respectively). Conclusions A higher proportion of total blastocysts and usable blastocysts can be obtained by LAH in LGCE, which may be beneficial to the outcome of the IVF/ICSI-ET cycle.

scholarly journals Developmental potential of surplus morulas with delayed and/or incomplete compaction after freezing-thawing procedures

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Ni-Chin Tsai ◽  
Yu-Ting Su ◽  
Yu-Ju Lin ◽  
Hsin-Ju Chiang ◽  
Fu-Jen Huang ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Live Birth ◽  
Formation Rate ◽  
Live Birth Rate ◽  
Preimplantation Genetic Screening ◽  
Poor Quality ◽  
Clinical Pregnancy ◽  
Clinical Pregnancy Rate ◽  
Blastocyst Formation ◽  
Developmental Potential

Abstract Background Morulas with delayed growth sometimes coexist with blastocysts. There is still limited evidence regarding the optimal disposal of surplus morulas. With the advancement of vitrification, the freezing-thawing technique has been widely applied to zygotes with 2 pronuclei, as well as embryos at the cleavage and blastocyst stages. The freezing of morulas, however, has rarely been discussed. The purpose of this study was to investigate whether these poor-quality and slow-growing morulas are worthy of cryopreservation. Methods This is a retrospective, observational, proof-of-concept study. A total of 1033 day 5/6 surplus morulas were cryopreserved from January 2015 to December 2018. The study included 167 women undergoing 180 frozen embryo transfer cycles. After the morulas underwent freezing-thawing procedures, their development was monitored for an additional day. The primary outcome was the blastocyst formation rate. Secondary outcomes were clinical pregnancy rate, live birth rate and abortion rate. Results A total of 347 surplus morulas were thawed. All studied morulas showed delayed compaction (day 5, n = 329; day 6, n = 18) and were graded as having low (M1, n = 54), medium (M2, n = 138) or high (M3, n = 155) fragmentation. The post-thaw survival rate was 79.3%. After 1 day in extended culture, the blastocyst formation rate was 66.6%, and the top-quality blastocyst formation rate was 23.6%. The day 5 morulas graded as M1, M2, and M3 had blastocyst formation rates of 88.9, 74.0, and 52.8% (p < 0.001), respectively, and the top-quality blastocyst formation rates were 64.8, 25.2, and 9.0% (p < 0.001), respectively. The clinical pregnancy rate was 33.6%. Conclusions The post-thaw blastocyst formation rate was satisfactory, with approximately one-half of heavily fragmented morulas (M3) developing into blastocysts. Most of the poor-quality morulas were worth to freeze, with the reasonable goal of obtaining pregnancy and live birth. This alternative strategy may be a feasible approach for coping with poor-quality surplus morulas in non-PGS (preimplantation genetic screening) cycles.

Experience of using time-lapse microscopy in IVF and ICSI programs

2020 ◽  
pp. 47-50
Author(s):  
N. V. Saraeva ◽  
N. V. Spiridonova ◽  
M. T. Tugushev ◽  
O. V. Shurygina ◽  
A. I. Sinitsyna
Keyword(s):  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Selection Procedure ◽  
Time Lapse ◽  
Single Embryo Transfer ◽  
Main Group ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

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