Comparative proteomic analysis of sulfur-oxidizing Acidithiobacillus ferrooxidans CCM 4253 cultures having lost the ability to couple anaerobic elemental sulfur oxidation with ferric iron reduction

2016 ◽  
Vol 167 (7) ◽  
pp. 587-594 ◽  
Author(s):  
Jiri Kucera ◽  
Ondrej Sedo ◽  
David Potesil ◽  
Oldrich Janiczek ◽  
Zbynek Zdrahal ◽  
...  
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Proteomic Analysis ◽  
Iron Reduction ◽  
Elemental Sulfur ◽  
Ferric Iron ◽  
Sulfur Oxidation ◽  
Comparative Proteomic Analysis ◽  
Sulfur Oxidizing ◽  
Elemental Sulfur Oxidation

Changes in Acidithiobacillus ferrooxidans Ability to Reduce Ferric Iron by Elemental Sulfur

2015 ◽  
Vol 1130 ◽  
pp. 97-100 ◽  
Author(s):  
Jiri Kucera ◽  
Eva Pakostova ◽  
Oldrich Janiczek ◽  
Martin Mandl
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Proteomic Analysis ◽  
Ferrous Iron ◽  
Iron Reduction ◽  
Elemental Sulfur ◽  
Ferric Iron ◽  
Sulfur Metabolism ◽  
Sulfur Oxidation ◽  
Quinone Reductase ◽  
Heterodisulfide Reductase

Ferric iron may act as a thermodynamically favourable electron acceptor during elemental sulfur oxidation byAcidithiobacillus ferrooxidansin extremely acidic anoxic environments. A loss of anaerobic ferric iron reduction ability has been observed in ferrous iron-grownA. ferrooxidansCCM 4253 after aerobic passaging on elemental sulfur. In this study, iron-oxidising cells aerobically adapted from ferrous iron to elemental sulfur were still able to anaerobically reduce ferric iron, however, following aerobic passage on elemental sulfur it could not. Preliminary quantitative proteomic analysis of whole cell lysates of the passage that lost anaerobic ferric iron-reducing activity resulted in 150 repressed protein spots in comparison with the antecedent culture, which retained the activity. Identification of selected protein spots by tandem mass spectrometry revealed physiologically important proteins including rusticyanin and outer-membrane cytochrome Cyc2, which are involved in iron oxidation. Other proteins were associated with sulfur metabolism such as sulfide-quinone reductase and proteins encoded by the thiosulfate dehydrogenase and heterodisulfide reductase complex operons. Furthermore, proteomic analysis identified proteins directly related to anaerobiosis. The results indicate the importance of iron-oxidising system components for anaerobic sulfur oxidation in the studied microbial strain.

Sulfur Oxidation and Coupled Iron Reduction at Low Temperatures

2007 ◽  
Vol 20-21 ◽  
pp. 584-584 ◽  
Author(s):  
Daniel Kupka ◽  
Mark Dopson ◽  
Olli H. Tuovinen
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Low Temperatures ◽  
Iron Reduction ◽  
Elemental Sulfur ◽  
Surface Soil ◽  
Sulfur Oxidation ◽  
Oxygen Limitation ◽  
Northern Siberia ◽  
Elemental Sulfur Oxidation

The purpose of this work was to characterize elemental sulfur oxidation by a psychrotrophic Acidithiobacillus ferrooxidans culture that originated from an AMD-impacted surface soil in a permafrost area in northern Siberia. In this work, the iron-oxidizing culture was cultivated with elemental sulfur with and without Fe2+ or Fe3+ in flasks on a shaker to avoid oxygen limitation.

Elemental Sulfur Oxidation by Thiobacillus spp. and Aerobic Heterotrophic Sulfur-Oxidizing Bacteria

Pedosphere ◽  
2010 ◽  
Vol 20 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Zhi-Hui YANG ◽  
K. STÖVEN ◽  
S. HANEKLAUS ◽  
B.R. SINGH ◽  
E. SCHNUG
Keyword(s):  
Elemental Sulfur ◽  
Sulfur Oxidation ◽  
Sulfur Oxidizing ◽  
Elemental Sulfur Oxidation ◽  
Sulfur Oxidizing Bacteria

scholarly journals Distinct Roles of Acidophiles in Complete Oxidation of High-Sulfur Ferric Leach Product of Zinc Sulfide Concentrate

2020 ◽  
Vol 8 (3) ◽  
pp. 386 ◽  
Author(s):  
Maxim Muravyov ◽  
Anna Panyushkina
Keyword(s):  
Microbial Community ◽  
Zinc Sulfide ◽  
Elemental Sulfur ◽  
Waste Product ◽  
Sulfur Oxidation ◽  
Low Grade ◽  
Sulfide Concentrates ◽  
Sulfide Concentrate ◽  
Elemental Sulfur Oxidation ◽  
Ferric Leaching

A two-step process, which involved ferric leaching with biologically generated solution and subsequent biooxidation with the microbial community, has been previously proposed for the processing of low-grade zinc sulfide concentrates. In this study, we carried out the process of complete biological oxidation of the product of ferric leaching of the zinc concentrate, which contained 9% of sphalerite, 5% of chalcopyrite, and 29.7% of elemental sulfur. After 21 days of biooxidation at 40 °C, sphalerite and chalcopyrite oxidation reached 99 and 69%, respectively, while the level of elemental sulfur oxidation was 97%. The biooxidation residue could be considered a waste product that is inert under aerobic conditions. The results of this study showed that zinc sulfide concentrate processing using a two-step treatment is efficient and promising. The microbial community, which developed during biooxidation, was dominated by Acidithiobacillus caldus, Leptospirillum ferriphilum, Ferroplasma acidiphilum, Sulfobacillus thermotolerans, S. thermosulfidooxidans, and Cuniculiplasma sp. At the same time, F. acidiphilum and A. caldus played crucial roles in the oxidation of sulfide minerals and elemental sulfur, respectively. The addition of L. ferriphilum to A. caldus during biooxidation of the ferric leach product proved to inhibit elemental sulfur oxidation.

Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans ?

2016 ◽  
Vol 167 (5) ◽  
pp. 357-366 ◽  
Author(s):  
Jiri Kucera ◽  
Eva Pakostova ◽  
Jan Lochman ◽  
Oldrich Janiczek ◽  
Martin Mandl
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Ferric Iron ◽  
Sulfur Oxidation ◽  
Anaerobic Sulfur Oxidation

scholarly journals Kinetic Constant Variability in Bacterial Oxidation of Elemental Sulfur

2007 ◽  
Vol 73 (11) ◽  
pp. 3752-3754 ◽  
Author(s):  
Blanka Pokorna ◽  
Martin Mandl ◽  
Sarka Borilova ◽  
Pavla Ceskova ◽  
Romana Markova ◽  
...  
Keyword(s):  
Acidithiobacillus Ferrooxidans ◽  
Elemental Sulfur ◽  
Kinetic Constant ◽  
Sulfur Oxidation ◽  
Growth Yields ◽  
Resting Cells ◽  
Michaelis Constant ◽  
Bacterial Oxidation ◽  
Batch Cultures ◽  
Oxidation Rates

ABSTRACT Wide ranges of growth yields on sulfur (from 2.4 × 1010 to 8.1 × 1011 cells g−1) and maximum sulfur oxidation rates (from 0.068 to 1.30 mmol liter−1 h−1) of an Acidithiobacillus ferrooxidans strain (CCM 4253) were observed in 73 batch cultures. No significant correlation between the constants was observed. Changes of the Michaelis constant for sulfur (from 0.46 to 15.5 mM) in resting cells were also noted.

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