Applications of 3D printed bone tissue engineering scaffolds in the stem cell field

There is a growing interest in developing 3D porous scaffolds with tunable architectures for bone tissue engineering. Surface topography has been shown to control stem cell behavior including differentiation. In this study, we printed 3D porous scaffolds with wavy or linear patterns to investigate the effect of wavy scaffold architecture on human mesenchymal stem cell (hMSC) osteogenesis. Five distinct wavy scaffolds were designed using sinusoidal waveforms with varying wavelengths and amplitudes, and orthogonal scaffolds were designed using linear patterns. We found that hMSCs attached to wavy patterns, spread by taking the shape of the curvatures presented by the wavy patterns, exhibited an elongated shape and mature focal adhesion points, and differentiated into the osteogenic lineage. When compared to orthogonal scaffolds, hMSCs on wavy scaffolds showed significantly enhanced osteogenesis, indicated by higher calcium deposition, alkaline phosphatase activity, and osteocalcin staining. This study aids in the development of 3D scaffolds with novel architectures to direct stem osteogenesis for bone tissue engineering.

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3D-printed β-TCP bone tissue engineering scaffolds: Effects of chemistry on in vivo biological properties in a rabbit tibia model

Journal of Materials Research ◽

10.1557/jmr.2018.233 ◽

2018 ◽

Vol 33 (14) ◽

pp. 1939-1947 ◽

Cited By ~ 13

Author(s):

Samit Kumar Nandi ◽

Gary Fielding ◽

Dishary Banerjee ◽

Amit Bandyopadhyay ◽

Susmita Bose

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Biological Properties ◽

Tissue Engineering Scaffolds ◽

Rabbit Tibia ◽

3D Printed ◽

Image Position

Abstract

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3D printed tricalcium phosphate bone tissue engineering scaffolds: effect of SrO and MgO doping on in vivo osteogenesis in a rat distal femoral defect model

Biomaterials Science ◽

10.1039/c3bm60132c ◽

2013 ◽

Vol 1 (12) ◽

pp. 1250 ◽

Cited By ~ 99

Author(s):

Solaiman Tarafder ◽

Neal M. Davies ◽

Amit Bandyopadhyay ◽

Susmita Bose

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tricalcium Phosphate ◽

Defect Model ◽

Tissue Engineering Scaffolds ◽

Femoral Defect ◽

3D Printed

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HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

Dentika Dental Journal ◽

10.32734/dentika.v19i2.457 ◽

2016 ◽

Vol 19 (2) ◽

pp. 93-100

Author(s):

Lalita El Milla

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Functional Groups ◽

Bone Growth ◽

Three Dimensional ◽

Dimensional Structure ◽

Tissue Engineering Scaffolds ◽

Hydroxyapatite Powder ◽

Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

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Study on the cytocompatibility, mechanical and antimicrobial properties of 3D printed composite scaffolds based on PVA/ Gold nanoparticles (AuNP)/ Ampicillin (AMP) for bone tissue engineering

Materials Today Communications ◽

10.1016/j.mtcomm.2021.102458 ◽

2021 ◽

pp. 102458

Author(s):

Aysenur Topsakal ◽

Swati Midha ◽

Esra Yuca ◽

Arı Tukay ◽

Hilal Turkoglu Sasmazel ◽

...

Keyword(s):

Tissue Engineering ◽

Gold Nanoparticles ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Antimicrobial Properties ◽

Composite Scaffolds ◽

3D Printed

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Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

Biomedical glasses ◽

10.1515/bglass-2020-0006 ◽

2020 ◽

Vol 6 (1) ◽

pp. 57-69

Author(s):

Amirhosein Fathi ◽

Farzad Kermani ◽

Aliasghar Behnamghader ◽

Sara Banijamali ◽

Masoud Mozafari ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Three Dimensional ◽

Layer By Layer ◽

Composite Scaffolds ◽

3D Printed ◽

Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

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