scholarly journals In vivo evaluation of microbial reduction after chemo-mechanical preparation of necrotic root canals with or without apical periodontitis

2014 ◽  
Vol 55 (2) ◽  
pp. 89-96
Author(s):  
Ana Moura Teles ◽  
M. Conceição Manso ◽  
Cristina Pina ◽  
José Cabeda
Keyword(s):  
Microbial Reduction ◽  
Apical Periodontitis ◽  
In Vivo Evaluation ◽  
Root Canals ◽  
Mechanical Preparation

scholarly journals Efficacy of Endodontic Treatment on the Persistence of Selected Endodontic Pathogens and on Radiographical Periapical Healing

2015 ◽  
Vol 17 (2) ◽  
pp. 43
Author(s):  
Rodolfo Zeledón DDS, Esp ◽  
Carolina Ballestero DDS, Esp ◽  
Erick Hernández DDS. Esp ◽  
Tatiana Ramírez DDS, MSD ◽  
Marianela Benavides DDS, Esp ◽  
...  
Keyword(s):  
Positive Impact ◽  
Fusobacterium Nucleatum ◽  
Dental Students ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Root Canals ◽  
Prevotella Nigrescens ◽  
Infected Root

The objective of this clinical study was to evaluate by polymerase chain reaction (PCR) the antimicrobial efficacy and the outcome of an endodontic protocol treatment (EPT) performed by under-graduated dental students on infected root canals associated with periapical lesions. Fifty-six patients attending for treatment of pulp necrosis and apical periodontitis were included. A specific EPT approach was performed and DNA extracts were taken at the baseline (S1), after a disinfection-neutralization procedure (S2), post-chemomechanical preparation (S3) and after Intracanal medication (S4) for the presence of specific bacteria. The outcome of the EPT was assessed radiographically using the Periapical Index-system (PAI) after 18-month follow-up. An intergroup evaluation shows that when comparing S1 to S3, S1 to S4, S2 to S3, and S2 to S4, the presence of bacteria were significantly reduced (P < 0.05). Finally, S4 was not significantly reduced when compared with S3 (P > 0.05). The most prevalent species in their respect order were Actinomyces Israelii > Enterococcus Faecalis > Fusobacterium Nucleatum/Prevotella Nigrescens > Phorphyromonas Endodontalis. After the 18-month follow-up, the overall success rate of root canal – treated teeth was of 88% (PAI 1 or 2). This In Vivo study demonstrated that EPT significantly reduced the number of cases with positive results for the studied bacteria, showing a profound positive impact in the outcome of endodontic treatment of teeth diagnosed with Pulp Necrosis and Apical Periodontitis. 

scholarly journals Effects of Contemporary Irrigant Activation Schemes and Subsequent Placement of an Interim Dressing on Bacterial Presence and Activity in Root Canals Associated with Asymptomatic Apical Periodontitis

2020 ◽  
Vol 9 (3) ◽  
pp. 854 ◽  
Author(s):  
Alexandre P. L. Carvalho ◽  
Laura C. L. Nardello ◽  
Fernanda S. Fernandes ◽  
Fernanda P. Bruno ◽  
Luiza R. Paz ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Quantitative Polymerase Chain Reaction ◽  
Apical Periodontitis ◽  
Universal Primers ◽  
Rrna Gene ◽  
Root Canal Preparation ◽  
Root Canals ◽  
Mechanical Preparation ◽  
Root Canal Disinfection

New tools for activating endodontic irrigants have evolved, yet their impact on root canal disinfection, in comparison to the passive placing of an inter-visit medication, have not yet been fully elucidated. The use of DNA- and rRNA-based methods may cast some new light on this issue, as they allow a comparison to be made between microbial presence and activity. Therefore, the aim of this single-arm intervention trial is to evaluate the antibacterial effect of endodontic procedures using both molecular methods. Root canal samples were obtained from 20 patients with asymptomatic apical periodontitis after each treatment step: access cavity, chemo-mechanical preparation, adjunctive procedures (XP-endo Finisher file and passive ultrasonic irrigation), calcium hydroxide medication, and 2nd-visit root canal preparation. DNA and cDNA from the samples were subjected to quantitative polymerase chain reaction with universal primers for the bacterial 16S rRNA gene. Chemo-mechanical preparation promoted a drastic reduction in bacterial levels and activity, whereas the adjunctive procedures did not make a significant contribution to further disinfection. At the 2nd visit, bacteria were active after the use of calcium hydroxide medication; however, they were significantly reduced after a 2nd-visit preparation. Consequently, the lowest bacterial levels were found at the end of the treatment. This clinical trial, which used an rRNA and rDNA combined approach, confirmed previous studies showing that root canal preparation represents the main strategy for root canal disinfection.

scholarly journals In Vivo Study of Calcium Hydroxide Endodontic Treatment in Chronic Apical Periodontitis

2018 ◽  
Vol 24 (4) ◽  
pp. 164-167
Author(s):  
Gheorghiu Irina-Maria ◽  
Mitran Loredana ◽  
M. Mitran ◽  
Temelcea Anca-Nicoleta ◽  
Scarlatesc Sanziana ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Essential Role ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Microbial Load ◽  
Root Canals

Abstract The aim of this study was to evaluate the in vivo efficiency of endodontic treatment with calcium hydroxide in chronic apical periodontitis by determining the microbial load from the endodontic space in different stages of treatment. The microbiological determinations that have been made have led to the conclusion that calcium hydroxide possesses a redoubltable antimicrobial activity, but only with the condition of long-lasting contact with the root canals. Another important result is the essential role of the rigorous biomechanical treatment of the root canals, intracanal medication with calcium hydroxide is addresses mostly to microorganisms located in root canal ramifications.

scholarly journals In Vivo Evaluation of Operative Torque Generated by Two Nickel-Titanium Rotary Instruments during Root Canal Preparation

2019 ◽  
Vol 13 (04) ◽  
pp. 556-562 ◽  
Author(s):  
Gianluca Gambarini ◽  
Massimo Galli ◽  
Marco Seracchiani ◽  
Dario Di Nardo ◽  
Marco A. Versiani ◽  
...  
Keyword(s):  
Root Canal ◽  
Peak Torque ◽  
Root Canal Preparation ◽  
Preparation Time ◽  
In Vivo Evaluation ◽  
Significance Level ◽  
Root Canals ◽  
Maxillary Premolars ◽  
Torque Values

Abstract Objectives This in vivo study evaluated the operative torque and preparation time of ProTaper NEXT (Dentsply Maillefer; Ballaigues, Switzerland) and EdgeFile X7 (EdgeEndo; Albuquerque, New Mexico, United States) rotary systems during root canal preparation of maxillary premolars. Materials and Methods Ten double-rooted maxillary premolars with independent canals were selected. Each canal in each tooth was prepared with one of the rotary systems (n = 10), ProTaper NEXT or EdgeFile X7. The instruments were rotated at 300 rpm with maximum torque set at 2 N.cm using an electric motor (KaVo; Biberach, Germany) that automatically recorded torque values at every 1/10th of a second (ds). Statistical Analysis Operative torque (N.cm) and preparation time (s) of the first shaping instrument (size 17/.04) of both rotary systems were recorded and statistically compared using the Mann–Whiney U test with a significance level set at 5%. Results No instrument exhibited flute deformation or underwent intracanal failure. No differences were found between the instruments regarding the maximum (peak) torque values (p > 0.05). EdgeFile X7 17/.04 required significantly less preparation time (3.75 seconds interquartile range [IQR]: 3.2–9.0) than ProTaper NEXT X1 (15.45 seconds IQR: 8.35–21.1) (p < 0.05). The median operative torque values of ProTaper NEXT X1 (0.26 N.cm; IQR: 0.18–0.49) were significantly higher compared with EdgeFile X7 17/.04 (0.09 N.cm; IQR: 0.05–0.17) (p < 0.05). Conclusions Although no difference was found between the median peak torque values of ProTaper NEXT X1 and EdgeFile X7 17/.04 instruments, the operative torque and instrumentation time results were impacted by their different designs and alloys during clinical preparation of root canals.

scholarly journals Effect of Different Irrigating Solutions and Photo-Activated Therapy for In Vivo Root Canal Treatment

2015 ◽  
Vol 26 (3) ◽  
pp. 228-233 ◽  
Author(s):  
Fernanda Ullmann López ◽  
Patrícia Maria Poli Kopper ◽  
Alvaro Della Bona ◽  
Liviu Steier ◽  
José Antônio Poli de Figueiredo ◽  
...  
Keyword(s):  
Root Canal ◽  
Qualitative Data ◽  
Saline Solution ◽  
Positive Control ◽  
Root Canal Treatment ◽  
Single Visit ◽  
Root Canals ◽  
Mechanical Preparation ◽  
Oral Environment

This study aimed to evaluate histologically the effect of irrigation with 400 ppm Sterilox, 2% and 5% sodium hypochlorite (NaOCl), with and without photo-activated therapy (PAD), in a single-visit root canal treatment of dog's teeth with apical periodontitis (AP). Ten dogs were randomly divided into two groups (n=5): with and without PAD, and the root canals into four subgroups, according to the irrigating solution: SX (400 ppm Sterilox), SH2 (2% NaOCl), SH5 (5% NaOCl) and SS (saline solution) as positive control. A total of 134 root canals were opened and left exposed to the oral environment for 14 days and then sealed for 60 days for AP induction. Then, root canals were treated according to each proposed disinfecting protocol and filled in the same session. After 120 days, the dogs were euthanized and the periapical inflammatory events were evaluated under light microscopy. Qualitative data were submitted to Kruskal-Wallis and Mann-Whitney tests (α=0.05). PAD did not produce significant differences in the scores for apical inflammation when used after chemo-mechanical preparation (p>0.05). The irrigating solutions SX, SH2 and SH5 without PAD were statistically different from SS (p<0.05) that presented the greatest scores for apical inflammation. PAD did not show any additional effect for the treatment of root canals with pulp necrosis and AP in a single visit and 400 ppm Sterilox may be considered an alternative to NaOCl in root canal treatment.

scholarly journals Microbial Reduction by Two Chemical-Mechanical Protocols in Primary Teeth with Pulp Necrosis and Periradicular Lesion - An In Vivo Study

2014 ◽  
Vol 25 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Thaisa Cezária Triches ◽  
Luciene Cristina de Figueiredo ◽  
Magda Feres ◽  
Sérgio Fernando Torres de Freitas ◽  
Gláucia Santos Zimmermann ◽  
...  
Keyword(s):  
Primary Teeth ◽  
Primary Root ◽  
Microbial Reduction ◽  
Treatment Groups ◽  
Single Visit ◽  
Root Canals ◽  
Endodontic Infection ◽  
Multiple Visit ◽  
Before And After

The objective of this study was to determine the efficacy of chemical-mechanical procedures of two endodontic protocols for septic content reduction of root canals from primary teeth with pulp necrosis and periradicular lesion. Twenty-four primary root canals with pulp necrosis and periradicular lesion were divided into two treatment groups (n=12): multiple-visit and single-visit protocols. Samples were collected using sterile paper points before and after endodontic cleaning followed by microbiological identification through checkerboard DNA-DNA hybridization. Statistical analysis was performed using Proportion Test for score=0 comparing the findings before and after treatment for each group (Wilcoxon's test) as well as the differences in scores between protocols (Mann-Whitney's test) (p<0.05). Data were expressed as prevalence (presence or absence) and estimate of the average count (x105 cells) of each species. Differences in proportions of score=0 prior to treatment were non-significant (p=0.415), demonstrating equivalence between groups. A significant increase in score=0 was detected after treatment for both groups (p<0.0001). Single-visit protocol achieved a significantly greater reduction in mean scoring following endodontic treatment (p=0.024). Both protocols were capable of significantly reducing septic content in root canals of primary teeth with periradicular lesion. Moreover, single-visit protocol showed greater efficacy in reducing endodontic infection.

An in vivo evaluation of the penetration of an irrigating solution in root canals

1977 ◽  
Vol 3 (10) ◽  
pp. 394-398 ◽  
Author(s):  
R. Martin Salzgeber ◽  
J. David Brilliant
Keyword(s):  
In Vivo Evaluation ◽  
Root Canals

scholarly journals Antibacterial effect of root canal preparation and calcium hydroxide paste (Calen) intracanal dressing in primary teeth with apical periodontitis

2005 ◽  
Vol 13 (4) ◽  
pp. 351-355 ◽  
Author(s):  
Gisele Faria ◽  
Paulo Nelson-Filho ◽  
Aldevina Campos de Freitas ◽  
Sada Assed ◽  
Izabel Yoko Ito
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Primary Teeth ◽  
Antibacterial Effect ◽  
Apical Periodontitis ◽  
Wilcoxon Test ◽  
Microbial Culture ◽  
Root Canal Preparation ◽  
Root Canals ◽  
Mechanical Preparation

The aim of this study was to evaluate the antibacterial action of root canal mechanical preparation using 2.5% sodium hypochlorite as the irrigating solution and a calcium hydroxide paste as the antibacterial intracanal dressing in human primary teeth root canals with pulp necrosis and apical periodontitis by means of microbial culture. A total of 26 root canals of human primary teeth with pulp necrosis and apical periodontitis were used. Samples were collected before, 72h after biomechanical treatment and 72h after removal of the intracanal dressing. Comparison by Wilcoxon test showed that root canal mechanical preparation effectively eliminated all microorganisms in 20% of the root canals, and the intracanal dressing in 62.5%; however, the cumulative action of biomechanical treatment and intracanal dressing eliminated the microorganisms of 70% of the root canals (p<0.001). Isolated root canal mechanical preparation presented poorer microbiological results that those obtained with root canal mechanical preparation and the use of an intracanal dressing indicating the necessity of topical application of an intracanal medication between sessions in primary teeth with pulp necrosis and apical periodontitis.

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