scholarly journals Microbial Reduction by Two Chemical-Mechanical Protocols in Primary Teeth with Pulp Necrosis and Periradicular Lesion - An In Vivo Study

2014 ◽  
Vol 25 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Thaisa Cezária Triches ◽  
Luciene Cristina de Figueiredo ◽  
Magda Feres ◽  
Sérgio Fernando Torres de Freitas ◽  
Gláucia Santos Zimmermann ◽  
...  
Keyword(s):  
Primary Teeth ◽  
Primary Root ◽  
Microbial Reduction ◽  
Treatment Groups ◽  
Single Visit ◽  
Root Canals ◽  
Endodontic Infection ◽  
Multiple Visit ◽  
Before And After

The objective of this study was to determine the efficacy of chemical-mechanical procedures of two endodontic protocols for septic content reduction of root canals from primary teeth with pulp necrosis and periradicular lesion. Twenty-four primary root canals with pulp necrosis and periradicular lesion were divided into two treatment groups (n=12): multiple-visit and single-visit protocols. Samples were collected using sterile paper points before and after endodontic cleaning followed by microbiological identification through checkerboard DNA-DNA hybridization. Statistical analysis was performed using Proportion Test for score=0 comparing the findings before and after treatment for each group (Wilcoxon's test) as well as the differences in scores between protocols (Mann-Whitney's test) (p<0.05). Data were expressed as prevalence (presence or absence) and estimate of the average count (x105 cells) of each species. Differences in proportions of score=0 prior to treatment were non-significant (p=0.415), demonstrating equivalence between groups. A significant increase in score=0 was detected after treatment for both groups (p<0.0001). Single-visit protocol achieved a significantly greater reduction in mean scoring following endodontic treatment (p=0.024). Both protocols were capable of significantly reducing septic content in root canals of primary teeth with periradicular lesion. Moreover, single-visit protocol showed greater efficacy in reducing endodontic infection.

COMPARISON OF MANUAL AND ROTARY INSTRUMENTATION TECHNIQUES FOR REMAINING DENTIN THICKNESS IN PRIMARY TEETH USING CBCT

2019 ◽  
Vol 1 (1) ◽  
pp. 01-06
Author(s):  
ANCHANA V ◽  
SHENVI AKSHATA AJAY ◽  
PRAHLAD GADICHERLA ◽  
DHANANJAYA G ◽  
PUSHPALATHA C
Keyword(s):  
Stainless Steel ◽  
Null Hypothesis ◽  
Primary Teeth ◽  
Primary Root ◽  
Root Canal Preparation ◽  
Cleaning Efficiency ◽  
Root Canals ◽  
Before And After ◽  
3D Software ◽  
Dentin Thickness

Aim: The purpose of this study was to compare the residual dentin thickness after manual and rotary instrumentation technique in primary root canals using CBCT. The null hypothesis was that there is no difference in the cleaning efficiency of both manual and rotary technique. Methodology: Manual filing was performed on 30 root canals using stainless steel S-files. Rotary instrumentation was carried out on a further 30 root canals, using rotary ProTaper files in a 300-rotation per minute (r.p.m.) Handpiece (NSK Endomate DT) according to the manufacturer’s instructions. The roots were aligned perpendicularly to the beam, and they were scanned before and after instrumentation by using the CBCT operating at 60 kV and 2.5mA. Pre-instrumentation and post-instrumentation measurements of canals were performed by using the Carestream 3D software. Results: When both the manual and rotary methods of canal instrumentation were compared, it was found that the reduction of dentinal thickness with respect to the manual method was more than the rotary method. Conclusions: There was more dentine thickness reduction in manual instrumentation than rotary instrumentation for root canal preparation in primary teeth.

Аpplying of high-frequency monopolar diathermocoagulation in the treatment of chronic pulpitis

2020 ◽  
Vol 1 (12) ◽  
pp. 40-42
Author(s):  
F. Yu. Daurova ◽  
D. I. Tomaeva ◽  
S. V. Podkopaeva ◽  
Yu. A. Taptun
Keyword(s):  
Root Canal ◽  
High Frequency ◽  
Antibacterial Effect ◽  
Comparison Group ◽  
Poor Quality ◽  
Endodontic Treatment ◽  
Root Canals ◽  
The Third ◽  
Before And After

Relevance: the reason for the development of complications in endodontic treatment is poor-quality instrumental treatment root canals.Aims: a study of the animicrobial action and clinical efficacy of high-frequency monopolar diathermocoagulation in the treatment of chronic forms of pulpitis.Materials and methods: 102 patients with various chronic forms of pulpitis were divided into three groups of 34 patients each. In the first two groups, high-frequency monopolar diathermocoagulation was used in endodontic treatment in different modes. In the third group, endodontic treatment was carried out without the use of diathermocoagulation (comparison group). The root canal microflora in chronic pulpitis in vivo was studied twice-before and after diathermocoagulation.Results: it was established that high-frequency monopolar diathermocoagulation in the effect mode is 3, power is 4 (4.1 W) and effect is 4, power is 4 (5.4 W) with an exposure time of 3 seconds, it has a pronounced antibacterial effect on all presented pathogenic microflora obtained from the root canals of the teeth.

scholarly journals In vitro evaluation of the effectiveness of the chemomechanical preparation against Enterococcus faecalis after single- or multiple-visit root canal treatment

2007 ◽  
Vol 21 (4) ◽  
pp. 308-313 ◽  
Author(s):  
Eduardo Diogo Gurgel-Filho ◽  
Nilton Vivacqua-Gomes ◽  
Brenda Paula Figueiredo de Almeida Gomes ◽  
Caio Cezar Randi Ferraz ◽  
Alexandre Augusto Zaia ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Control Groups ◽  
Single Visit ◽  
Root Canals ◽  
Multiple Visit ◽  
Microbial Samples ◽  
Chlorhexidine Gel

The purpose was to assess the elimination of Enterococcus faecalis in vitro in human mandibular premolars after chemomechanical preparation with or without the use of a calcium hydroxide dressing. After 60 days of contamination with E. faecalis, the root canals were prepared using the Crown-Down technique combined with 2% chlorhexidine gel irrigation. Then, the specimens were divided into two experimental groups, treated in a single visit or in multiple visits, and two control groups. The multiple-visit group received a dressing with calcium hydroxide for 14 days (CalenTM) and the single-visit group did not receive any medication. In the two control groups, the canals were filled with BHI after chemomechanical preparation with 2% chlorhexidine gel or distilled water. Microbial samples were taken from the root canals for colony forming unit count for each phase of the treatment using sterile paper points inside the root canal lumen. Data were ranked and analyzed by the Kruskal-Wallis statistical test. The residual microbial colonies were then assessed. The results showed that chemomechanical preparation using 2% chlorhexidine gel with no intra-canal dressing reduced by 100% the E. faecalis contamination of the root canal lumen. The calcium-hydroxide group that received the 14-day intra-canal dressing allowed a small number of bacteria to grow between visits, but without statistical differences between groups.

Comparative Analysis of Root Dentin Morphology and Structure of Human Versus Bovine Primary Teeth

2015 ◽  
Vol 21 (3) ◽  
pp. 689-694 ◽  
Author(s):  
Bruna M. Costa ◽  
Alexsandra S. Iwamoto ◽  
Regina M. Puppin-Rontani ◽  
Fernanda M. Pascon
Keyword(s):  
Repeated Measures ◽  
Primary Teeth ◽  
Primary Root ◽  
Repeated Measures Anova ◽  
Root Canals ◽  
Morphology And Structure ◽  
Root Dentin ◽  
Dentin Tubules ◽  
Morphological Differences ◽  
Scanning Electron

AbstractThis study evaluated the structural and morphological differences between human and bovine primary root canals. Primary human maxillary central incisors (H) (n=9) and primary bovine incisors (B) (n=9) were selected. The roots were sectioned in the vestibular-lingual direction, planed and delimited in cervical, middle, and apical thirds. Tubule density (number of tubules per mm2) and diameter were analyzed by scanning electron microscopy (1,000 and 5,000×) using Image J 1.47 software. Data were submitted to two-way repeated measures ANOVA and Tukey tests (α=0.05). The highest tubule density was observed for B (28.527±1.717 mm2) compared with H (15.931±0.170 mm2) (p<0.01). Regarding root thirds, the cervical third presented a greater tubule density (26.417±11.654 mm2) than the apical third (17.999±5.873 mm2). The diameter of the dentin tubules was not different for cervical (3.50±0.08 µm), middle (3.45±0.30 µm) and apical thirds (3.42±0.33 µm) and substrate (H—3.29±0.14 µm; B—3.63±0.06 µm). It could be concluded that: (1) the radicular dentin structure of human and bovine primary teeth and root thirds differ in terms of the tubule density; (2) the radicular dentin morphology of human and bovine primary teeth and root thirds are similar in terms of the diameter of the dentin tubules.

β2-Adrenoceptor desensitization in human alveolar macrophages induced by inhaled terbutaline in vivo is not counteracted by budesonide

2001 ◽  
Vol 100 (4) ◽  
pp. 451-457 ◽  
Author(s):  
Anita ZETTERLUND ◽  
Paul HJEMDAHL ◽  
Kjell LARSSON
Keyword(s):  
Alveolar Macrophages ◽  
Control Group ◽  
Transcriptional Level ◽  
Down Regulation ◽  
Bronchodilator Response ◽  
Treatment Groups ◽  
Before And After ◽  
Β2 Agonist

In vitro studies suggest that glucocorticoids may counteract β-agonist-induced desensitization of β-adrenoceptors by actions at the transcriptional level, but the clinical relevance of such findings is not clear. Oral terbutaline treatment decreases β-adrenoceptor sensitivity in alveolar macrophages in vivo. This effect is not counteracted by inhaled or orally taken steroids. We therefore examined whether inhaled terbutaline elicited a similar effect on β2-adrenoceptor sensitivity in alveolar macrophages, and if co-treatment with an inhaled steroid, budesonide, would prevent such down-regulation. Bronchoalveolar lavage (BAL) and lung function tests, including bronchodilator responses to inhaled terbutaline, were performed before and after 2 weeks of regular inhalation of terbutaline, 0.5 mg three times daily, and budesonide, 400 µg twice daily, or placebo, in 24 healthy volunteers. Four untreated subjects served as controls. A marked, approx. 90%, decrease in isoprenaline-induced cAMP accumulation in alveolar macrophages was found in both treatment groups after 2 weeks, with no difference between placebo and budesonide (P = 0.45). In the untreated control group, cAMP responses to both isoprenaline and prostaglandin E1 tended to be lower on the second occasion. A limited, non-specific desensitization of adenylate cyclase activity thus contributed to the marked desensitization elicited by terbutaline inhalations. The bronchodilator response to inhaled terbutaline did not change after treatment in any of the three groups (F = 0.9, P = 0.50). In conclusion, inhalation of a β-agonist induced marked down-regulation of β2-adrenoceptor sensitivity in alveolar macrophages in vivo without influencing the bronchodilator response to a β2-agonist in healthy subjects. Co-treatment with an inhaled steroid failed to counteract the desensitization of alveolar macrophage β2-adrenoceptors.

scholarly journals Antibacterial Efficacy of Different Herbal Based Irrigant Solutions in Deciduous Teeth

2021 ◽  
Author(s):  
Naamatullah Ahmed Mohammed ◽  
Bahar Jaafar Selivany
Keyword(s):  
Green Tea ◽  
Normal Saline ◽  
Primary Teeth ◽  
Primary Root ◽  
Negative Control ◽  
Deciduous Teeth ◽  
Antibacterial Efficacy ◽  
Endodontic Infection ◽  
Significant Difference

Introduction: Endodontic infection requires the successful removal of microorganisms from the root canal system. The most effective irrigant solution is sodium hypochlorite, but possible problems due to its toxicity require the look for new alternatives. Constant increases in antibiotic resistance and side-effects caused by chemical irrigation have shifted research towards the production of herbal alternatives, especially in paediatric dentistry. Aim: To evaluate and compare the antibacterial efficacy of miswak, green tea, chamomile and 0.5% NaOCl as an endodontic irrigants against Enterococcus faecalis in primary root canals. Materials and Methods: The in-vitro study was conducted at the Department of Conservative Dentistry and Department of Microbiology, College of Dentistry, University of Duhok from January 2020 till June 2020. Seventy five freshly extracted intact human mandibular primary molars were decoronated at Cemento-Enamel Junction (CEJ) and distal roots were separated and biomechanically prepared up to F3 Protaper file and stored in normal saline. The specimens were inoculated with Enterococccus faecalis suspension and incubated for 72 hours. Specimens were divided into five groups containing fifteen teeth each (n=15). Freshly prepared alcoholic extracts of miswak, green tea and chamomile were used as an irrigant solution against E.faecalis compared to NaOCl as positive control and normal saline as negative control. Swabs were collected using F3 protaper paper points. The number of colonies was counted in suitable plate under good illumination and manual lens for magnification. Statistical analysis was performed by using Kruskal-Wallis one-way Analysis and Student-Newman-Keuls Method. Statistical Package for the Social Sciences (SPSS) version 26.0 was used. The p-values more than 0.05 were considered as statistically non significant. Results: Green Tea, among the herbal experimental groups, had the most effective antibacterial effect against E.faecalis. No significant statistical difference was detected between green tea (p=0.272) and NaOCl; however, there was significant difference between miswak, chamomile and NaOCl as well as between the rest herbal experimental groups and normal saline. Conclusion: NaOCl remains the gold standard as irrigant in primary teeth. Green tea extract may help in reducing E.faecalis inside the canals of primary teeth. Good efficacy against E.faecalis was also shown by Miswak and chamomile however the results obtained were not significant when compared with NaOCl.

scholarly journals Single-visit versus multiple-visits endodontic treatment of apical periodontitis: A meta-analysis of outcome assessed by intra oral radiographs

2019 ◽  
Author(s):  
Bahar Dehghan Manshadi ◽  
Asgeir Bårdsen
Keyword(s):  
Root Canal ◽  
Null Hypothesis ◽  
Meta Analysis ◽  
Primary Root ◽  
Apical Periodontitis ◽  
Root Canal Treatment ◽  
Treatment Modalities ◽  
National Library ◽  
Single Visit ◽  
Multiple Visit

Abstract The established multiple-visit treatment for necrotic teeth with apical periodontitis has been challenged by single-visit treatment. However, there is no clear support for the one or the other treatment in the literature. The aim of this meta-analysis was to investigate if non-surgical primary root canal treatment carried out in single-visit or multiple-visit makes any difference in terms of radiographic healing in necrotic teeth with apical periodontitis. The null hypothesis was: there is no difference in the rate of apical healing for the two treatment modalities The literature search was organized through the National Centre for Biotechnology Information (NCBI) at the U.S. National Library of Medicine (NLM the National Library of Medicine (NLM)) using PubMed database and EMBASE Ovid to select articles with single-visit or multiple-visit included teeth with pulp necrosis and apical periodontitis. Of the 60 identified studies, only eight studies were included in current meta-analysis. The main reasons for exclusions were teeth with other diagnosis except necrotic pulp and apical periodontitis, studies with no comparison between single- and multiple-visit endodontics within the same study and studies without healing rate appearance. Results from each of the included studies were entered into the statistical program Stata, Version 15 (Texas, USA). The odds ratio (OR) and the 95% confidence interval for each study were calculated (dependent variable: single-visit/multiple-visit, independent variables: healed/not healed based on intra oral radiographs) with the random effect model. There was no significant difference in radiographic evidence of periapical healing between single-visit endodontics and multiple-visit treatment modalities, of the primary root canal treatment in necrotic teeth with apical periodontitis (OR = 1.10, 95% CI: 0.67-1.83) as evaluated on intra oral radiographs. Thus, the null hypothesis could not be rejected.

scholarly journals Effect of Different Irrigating Solutions and Photo-Activated Therapy for In Vivo Root Canal Treatment

2015 ◽  
Vol 26 (3) ◽  
pp. 228-233 ◽  
Author(s):  
Fernanda Ullmann López ◽  
Patrícia Maria Poli Kopper ◽  
Alvaro Della Bona ◽  
Liviu Steier ◽  
José Antônio Poli de Figueiredo ◽  
...  
Keyword(s):  
Root Canal ◽  
Qualitative Data ◽  
Saline Solution ◽  
Positive Control ◽  
Root Canal Treatment ◽  
Single Visit ◽  
Root Canals ◽  
Mechanical Preparation ◽  
Oral Environment

This study aimed to evaluate histologically the effect of irrigation with 400 ppm Sterilox, 2% and 5% sodium hypochlorite (NaOCl), with and without photo-activated therapy (PAD), in a single-visit root canal treatment of dog's teeth with apical periodontitis (AP). Ten dogs were randomly divided into two groups (n=5): with and without PAD, and the root canals into four subgroups, according to the irrigating solution: SX (400 ppm Sterilox), SH2 (2% NaOCl), SH5 (5% NaOCl) and SS (saline solution) as positive control. A total of 134 root canals were opened and left exposed to the oral environment for 14 days and then sealed for 60 days for AP induction. Then, root canals were treated according to each proposed disinfecting protocol and filled in the same session. After 120 days, the dogs were euthanized and the periapical inflammatory events were evaluated under light microscopy. Qualitative data were submitted to Kruskal-Wallis and Mann-Whitney tests (α=0.05). PAD did not produce significant differences in the scores for apical inflammation when used after chemo-mechanical preparation (p>0.05). The irrigating solutions SX, SH2 and SH5 without PAD were statistically different from SS (p<0.05) that presented the greatest scores for apical inflammation. PAD did not show any additional effect for the treatment of root canals with pulp necrosis and AP in a single visit and 400 ppm Sterilox may be considered an alternative to NaOCl in root canal treatment.

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