Chemometric Spectrophotometric Methods for Simultaneous Estimation of Metoprolol Succinate and Amlodipine Besylate in Their Tablet Formulation

2021 ◽  
pp. 119641
Author(s):  
Amira H. Kamal ◽  
Sherin F. Hammad ◽  
Doaa N. Kamel
Keyword(s):  
Tablet Formulation ◽  
Simultaneous Estimation ◽  
Amlodipine Besylate ◽  
Metoprolol Succinate ◽  
Spectrophotometric Methods

Stability Indicating HPTLC Method for Simultaneous Determination of Amlodipine Besylate and Lisinopril in Combined Dose Tablet Formulation

2021 ◽  
pp. 6250-6256
Author(s):  
Sagar B. Wankhede ◽  
Deepak S. Khobragade ◽  
Sukeshini B. Lote ◽  
S. Patil
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Degradation Products ◽  
Cost Effective ◽  
Tablet Formulation ◽  
Simultaneous Estimation ◽  
Amlodipine Besylate ◽  
Stability Indicating ◽  
Limit Of Quantitation ◽  
Dose Tablet

A combined dose tablet formulation containing Amlodipine besylate and Lisinopril is used for the treatment of essential hypertension. The present study reports development and validation of stability indicating high performance thin layer chromatographic method for simultaneous estimation of these drugs in combined dose tablet formulation. The two drugs were satisfactorily resolved on aluminum plates precoated with silica gel 60F254 using n-butanol : methanol: ammonia (4:4:1 v/v/v) as mobile phase. The Rf value for lisinopril and amlodipine besylate were 0.27±0.02 and 0.62±0.02, respectively. Densitometric evaluation of the separated bands was performed at 215nm. The calibration curves for lisinopril and amlodipine besylate were found to be linear in the concentration range of 1000-6000ng/band. The method was validated as per ICH guidelines for accuracy, precision, robustness, specificity, limit of detection and limit of quantitation. Statistical analysis proves that the method is suitable for simultaneous analysis of Lisinopril and Amlodipine besylate in pharmaceutical formulation without any interference from the excipients/degradant. The developed method offers several advantages such as sensitive, rapid, cost effective and less time consuming as compared to the reported methods. As the method could effectively separate the drugs from its degradation products, it can be employed as a stability indicating method.

scholarly journals Method Development and Validation of simultaneous estimation for Ramipril and Amlodipine besylate by UV- VISIBLE Spectrophotometric method

2020 ◽  
Vol 11 (3) ◽  
pp. 3828-3832
Author(s):  
Khairunnisa Tasneem ◽  
Sonia K ◽  
Lakshmi K S
Keyword(s):  
Method Development ◽  
Acid Reflux ◽  
Dose Assessment ◽  
Fixed Dose ◽  
Simultaneous Estimation ◽  
Distilled Water ◽  
Amlodipine Besylate ◽  
Spectrophotometric Methods ◽  
Label Claim ◽  
Uv Visible

This experiment shares the method development and analytical validation of an original, accurate and correct UV-Visible Spectrophotometric methods for the fixed dose assessment of Ramipril and Amlodipine besylate. The working solutions of Telmisartan and Ramipril were scanned at 240 nanometer and 210nanometer respectively. The regression strength of Amlodipine besylate and Ramipril over its absorbances take place as y=0.4291x0.0084 and y=0.0399x-0.310 respectively with a correlation coefficient (r2 ) of 0.9998 for Amlodipine besylate and 0.9993 for Ramipril. The intra-day precision in addition inter-day precision for Amlodipine besylate and its % RSD were obtained as 0.08% and 0.25% respectively. The intra-day precision in addition interday precision for Ramipril and% RSD were obtained as 0.16% and 0.24% respectively. The precise amount of tablet formulation were added which holds Alkaline (0.1 N Sodium hydroxide), Acidic (0.1 N Hydrochloric acid) reflux for 3 hours, 3% Oxydol at 50ºC, heat (60ºC), humidity (75 percentage Relative humidity) for 24 hr. and after the particular time diluted to distilled water, separated using Filter paper. From this stock solution, 5 mL section of the filtrate was pipetted out and further thinned with distilled water in a 100 mL standard flask (10 µg/mL). The standard stock solution of two drugs were prepared and compared against a label claim

scholarly journals Simultaneous Spectrophotometric Estimation of Telmisartan and Amlodipine Besylate in Tablet Dosage Form

2015 ◽  
Vol 3 (03) ◽  
pp. 50-54 ◽  
Author(s):  
N. K. Gupta ◽  
A. Peepliwal ◽  
D. S. Rathore ◽  
P. Gupta
Keyword(s):  
Quality Control ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Amlodipine Besylate ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Tablet Dosage ◽  
Routine Quality Control

A simple, accurate and reproducible spectrophotometric methods have been developed for the simultaneous estimation of Telmisartan (TEL) and Amlodipine Besylate (AML) in combined tablet dosage forms. The method involves determination using the simultaneous equation method, the sampling wavelengths selected are ‘TLM’ = 297nm.and ‘AML’ =238nm., over the concentration ranges of 8-48μg/ml for ‘TEL’ and 1-6 μg/ml for ‘AML’ respectively. The method was validated for linearity, accuracy, precision, robustness and application for assay as per ICH guidelines. The proposed method is simple, economical, accurate and precise, and could be successfully employed in routine quality control for the simultaneous analysis of Telmisartan (TEL) and Amlodipine Besylate (AML).

scholarly journals Simultaneous Estimation of Domperidone and Pantoprazole in Solid Dosage Form by UV Spectrophotometry

2006 ◽  
Vol 3 (3) ◽  
pp. 142-145
Author(s):  
P. Ravi Kumar ◽  
P. Bhanu Prakash ◽  
M. Murali Krishna ◽  
M. Santha Yadav ◽  
C. Asha Deepthi
Keyword(s):  
Simultaneous Equation ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Q Value ◽  
Value Analysis ◽  
Spectrophotometric Methods ◽  
Solid Dosage ◽  
Tablet Dosage

Domperidone is an antiemetic and pantoprazole is an antiulcer drug. Simple, precise, rapid and selective simultaneous equation and Q- analysis UV spectrophotometric methods have been developed for the simultaneous determination of domperidone and pantoprazole from combined tablet dosage forms. The methods involve solving of simultaneous equations and Q-value analysis based on measurement absorptivity at 216, 287 and 290 nm respectively. Linearity lies between 1-15 mcg/mL for domperidone and 0-50 mcg/mL for pantoprazole.

scholarly journals Three Different Spectrophotometric Methods for Simultaneous Determination of Pyriproxyfen and Chlorothalonil Residues in Cucumber and Cabbage Samples

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Shilan A. Omer ◽  
Nabil A. Fakhre
Keyword(s):  
Simultaneous Determination ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
The Third ◽  
Mean Centering ◽  
Relative Standard ◽  
One Way Anova

In this study, three simple and accurate spectrophotometric methods for simultaneous determination of pyriproxyfen and chlorothalonil residues in cucumbers and cabbages grown in experimental greenhouse were studied. The first method was based on the zero-crossing technique measurement for first and second derivative spectrophotometry. The second method was based on the first derivative of the ratio spectra. However, the third method was based on mean centering of ratio spectra. These procedures lack any previous separation steps. The calibration curves for three spectrophotometric methods are linear in the concentration range of 1–30 μg·mL−1 and 0.5–7 μg·mL−1 for pyriproxyfen and chlorothalonil successively. The recoveries ranged from 82.12–97.40% for pyriproxyfen and 81.51–97.04% for chlorothalonil with relative standard deviations less than 4.95% and 5.45% in all instances for pyriproxyfen and chlorothalonil, respectively. The results obtained from the proposed methods were compared statistically by using one-way ANOVA, and the results revealed there were no significant differences between ratio spectra and mean centering methods with the zero-crossing technique. The proposed methods are successfully applied for the simultaneous estimation of the residue of both pesticides in cucumber and cabbage samples.

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