Characterization of Prunus domestica L. in vitro regeneration via hypocotyls

2007 ◽  
Vol 112 (4) ◽  
pp. 462-466 ◽  
Author(s):  
Lining Tian ◽  
Susan Sibbald ◽  
Jayasankar Subramanian ◽  
Antonet Svircev
Keyword(s):  
In Vitro Regeneration ◽  
Prunus Domestica

scholarly journals In vitro regeneration and molecular characterization of Jatropha curcas plant

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Mohamed El-Sayed ◽  
Usama I. Aly ◽  
Mervat S. Mohamed ◽  
Mohamed R. Rady
Keyword(s):  
Molecular Characterization ◽  
Jatropha Curcas ◽  
In Vitro Regeneration

In vitro regeneration and cytological characterization of shoots from leaf explants of three accessions of Solanum commersonii

1993 ◽  
Vol 34 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Teodoro Cardi ◽  
Vittoria Iannamico ◽  
Fabrizio D'Ambrosio ◽  
Edgardo Filippone ◽  
Paul F. Lurquin
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Solanum Commersonii

scholarly journals In vitro regeneration and molecular characterization of some varieties of Lycopersicon esculentum Mill. in Bangladesh

2019 ◽  
Vol 54 (2) ◽  
pp. 117-124
Author(s):  
M Billah ◽  
TA Banu ◽  
M Islam ◽  
NA Banu ◽  
S Khan ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Molecular Characterization ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Cotyledonary Node ◽  
Cotyledonary Leaf ◽  
Lycopersicon Esculentum Mill ◽  
Regeneration Response

An efficient regeneration protocol was established for two varieties (BARI tomato-9 and BARI tomato-15) of tomato (Lycopersicon esculentum Mill.) using three explants namely cotyledonary node, cotyledonary leaf and hypocotyls. Among the three explants, maximum number of shoots was produced from cotyledonary leaf explants of BARI tomato-15 on MS with 2.0 mg/l BAP and 0.5 mg/l IAA. In this combination of BAP and IAA 86%, on an average, cotyledonary leaf explants showed regeneration response 14.12 shoots/explants. Explants from hypocotyl showed best results in MS medium with 2.0 mg/l BAP and 0.2 mg/l IAA in both the varieties. In case of cotyledonary node, BARI tomato-15 showed 6.0 shoot/explant on MS with 2.0 mg/l BAP and 1.0 mg/l IAA. Molecular characterization of total ten varieties of tomato in Bangladesh was done by using six arbitrary oligonucleotide RAPD primers. A total of 140 bands were produced where the highest genetic distance (0.6769) was found between BARI tomato-3 and Mintoo tomato and lowest distance (0.1035) was observed between BARI tomato-7 and BARI tomato-8. This result will be useful for designing future breeding programs. Bangladesh J. Sci. Ind. Res.54(2), 117-124, 2019

scholarly journals Influencing factors and structural characterization of hyperhydricity of in vitro regeneration in Brassica oleracea var. italica

2011 ◽  
Vol 91 (1) ◽  
pp. 159-165 ◽  
Author(s):  
Ya Yu ◽  
Yong-Qin Zhao ◽  
Bing Zhao ◽  
Shuxin Ren ◽  
Yang-Dong Guo
Keyword(s):  
Electron Microscopy ◽  
Brassica Oleracea ◽  
Influencing Factors ◽  
Structural Characterization ◽  
Indoleacetic Acid ◽  
Sucrose Concentration ◽  
In Vitro Regeneration ◽  
Transmission Electron

Yu, U., Zhao, Y.-Q., Zhao, B., Ren, S. and Guo, Y.-D. 2011. Influencing factors and structural characterization of hyperhydricity of in vitro regeneration in Brassica oleracea var. italica. Can. J. Plant Sci. 91: 159–165. This study examines factors that affect the occurrence of hyperhydric tissue in in vitro cultures of Brassica oleracea variety italica. The anatomy of normal and hyperhydric leaves of plantlets regenerated from the hypocotyls was compared using scanning electron microscopy and transmission electron microscopy. In hyperhydric leaves palisade tissue was absent and the spongy mesophyll displayed large, unorganized intercellular spaces. Hyperhydric leaves had abnormal stomata with deformed guard cells. Significant ultrastructural differences were observed between chloroplasts in normal and hyperhydric leaves. The effects of zeatin, indoleacetic acid, silver nitrate and sucrose on the formation of hyperhydric shoots were studied. Zeatin was the most important factor, followed by sucrose concentration, AgNO3 and indoleacetic acid. The process of hyperhydricity was found to be reversed by increasing the agar concentration and eliminating NH4NO3 from the macro-elements in the MS medium. This is the first report of hyperhydricity in Brassica oleracea, and our study gives a better understanding of the factors that influence hyperhydricity during in vitro regeneration in Brassica crops.

Assays for Phospholipid-Dependent Formation of Thrombin and Xa: A Potential Method for Quantifying Lupus Anticoagulant Activity

1991 ◽  
Vol 66 (04) ◽  
pp. 453-458 ◽  
Author(s):  
John T Brandt
Keyword(s):  
Specific Activity ◽  
Anticoagulant Activity ◽  
Factor Xa ◽  
Clinical Importance ◽  
Potential Method ◽  
Quantitative Expression ◽  
Increased Risk ◽  
Apparent Association

SummaryLupus anticoagulants (LAs) are antibodies which interfere with phospholipid-dependent procoagulant reactions. Their clinical importance is due to their apparent association with an increased risk of thrombo-embolic disease. To date there have been few assays for quantifying the specific activity of these antibodies in vitro and this has hampered attempts to purify and characterize these antibodies. Methods for determining phospholipid-dependent generation of thrombin and factor Xa are described. Isolated IgG fractions from 7 of 9 patients with LAs were found to reproducibly inhibit enzyme generation in these assay systems, permitting quantitative expression of inhibitor activity. Different patterns of inhibitory activity, based on the relative inhibition of thrombin and factor Xa generation, were found, further substantiating the known heterogeneity of these antibodies. These systems may prove helpful in further purification and characterization of LAs.

Characterization of the Original Christmas Disease Mutation (Cysteine 206 → Serine): From Clinical Recognition to Molecular Pathogenesis

1992 ◽  
Vol 67 (01) ◽  
pp. 063-065 ◽  
Author(s):  
Sherryl A M Taylor ◽  
Jacalyn Duffin ◽  
Cherie Cameron ◽  
Jerome Teitel ◽  
Bernadette Garvey ◽  
...  
Keyword(s):  
Nucleotide Substitution ◽  
Novel Mutation ◽  
Factor Ix ◽  
Causative Mutation ◽  
Coding Region ◽  
Body Of Knowledge ◽  
Polymerase Chain ◽  
Splice Junctions

SummaryChristmas disease was first reported as a distinct clinical entity in two manuscripts published in 1952 (1, 2). The eponym associated with this disorder, is the surname of the first patient examined in detail and reported by Biggs and colleagues in a paper describing the clinical and laboratory features of seven affected individuals (3). This patient has severe factor IX coagulant deficiency (less than 0.01 units/ml) and no detectable circulating factor IX antigen (less than 0.01 units/ml). Coding sequence and splice junctions of the factor IX gene from this patient have been amplified in vitro through the polymerase chain reaction (PCR). One nucleotide substitution was identified at nucleotide 30,070 where a guanine was replaced by a cytosine. This mutation alters the amino acid encoded at position 206 in the factor IX protein from cysteine to serine. The non conservative nature of this substitution, the absence of this change in more than 200 previously sequenced factor IX genes and the fact that the remainder of the coding region of this gene was normal, all provide strong circumstantial evidence in favour of this change being the causative mutation in this patient. The molecular characterization of this novel mutation in the index case of Christmas disease, contributes to the rapidly expanding body of knowledge pertaining to Christmas disease pathogenesis.

Sign in / Sign up

Export Citation Format

Share Document