scholarly journals Urinary Concentrations of Parabens and Serum Hormone Levels, Semen Quality Parameters, and Sperm DNA Damage

2011 ◽  
Vol 119 (2) ◽  
pp. 252-257 ◽  
Author(s):  
John D. Meeker ◽  
Tiffany Yang ◽  
Xiaoyun Ye ◽  
Antonia M. Calafat ◽  
Russ Hauser
Keyword(s):  
Dna Damage ◽  
Semen Quality ◽  
Quality Parameters ◽  
Hormone Levels ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Serum Hormone

scholarly journals Human Semen Quality, Sperm DNA Damage, and the Level of Urinary Concentrations of 1N and TCPY, the Biomarkers of Nonpersistent Insecticides

2018 ◽  
Vol 13 (1) ◽  
pp. 155798831881659 ◽  
Author(s):  
Emila Dziewirska ◽  
Michał Radwan ◽  
Bartosz Wielgomas ◽  
Anna Klimowska ◽  
Paweł Radwan ◽  
...  
Keyword(s):  
Dna Damage ◽  
Environmental Exposure ◽  
Semen Quality ◽  
Semen Analysis ◽  
Positive Association ◽  
Quality Parameters ◽  
Normal Morphology ◽  
Sperm Dna Damage ◽  
Fragmentation Index ◽  
Sperm Dna

The aim of the study was to evaluate the association between environmental exposure to nonpersistent insecticides and semen quality (concentration, motility, morphology, computer-aided semen analysis [CASA] parameters, and sperm DNA damage). Urine samples ( n = 315) collected from men who attended the infertility clinic with normal semen concentration of 15 to 300 mln/ml and age under 45 years were analyzed for two metabolites (1-naphthol [1N] and 3,5,6-trichloro-2-pyridinol [TCPY]) of nonpersistent insecticides. Participants provided semen, blood, and saliva samples; additionally, men filled a detailed questionnaire. The results identified that urinary TCPY concentration was significantly associated with a decrease in motility; also there was a positive association between TCPY and DNA fragmentation index (DFI). 1N concentration was negatively associated with a percentage of sperm with normal morphology and positively with one of the CASA parameters (curvilinear velocity [VCL]). The results suggest that environmental exposure to nonpersistent insecticides may have an impact on semen quality parameters and sperm DNA damage.

Effect of cooled storage on quality and DNA integrity of Asian elephant (Elephas maximus) spermatozoa

2012 ◽  
Vol 24 (8) ◽  
pp. 1105 ◽  
Author(s):  
P. Imrat ◽  
S. Mahasawangkul ◽  
J. Gosálvez ◽  
P. Suthanmapinanth ◽  
P. Sombutputorn ◽  
...  
Keyword(s):  
Semen Quality ◽  
Storage Conditions ◽  
Quality Parameters ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Dna Stability ◽  
Subjective Impression ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Rate Of Increase

Artificial insemination (AI) is a potentially useful tool for breeding captive elephants because it facilitates efforts to minimise inbreeding. However, cooled storage of elephant semen markedly reduces fertility. This study compared the effects on semen-quality parameters, including sperm DNA fragmentation, of storing elephant semen at 4°C or 15°C in a commonly-used diluent (TEST) or a diluent developed to protect against sperm DNA damage (BullMax). Storing elephant semen for >24 h in either extender at either temperature resulted in decreases in sperm motility, viability, acrosome integrity and DNA integrity (P < 0.05); the decrease in motility was especially rapid. A subjective impression of circular sperm movement in TEST was confirmed by a higher curvilinear velocity and amplitude of lateral head displacement, but lower straight-line velocity and linearity than in BullMax. Initial percentages of spermatozoa with fragmented DNA (%SDF) did not differ between extenders or temperatures, but the rate of increase in %SDF during a 48-h incubation at 37°C was higher in TEST than in BullMax (P < 0.05). In conclusion, BullMax allows more linear movement and better preserves DNA stability of stored elephant spermatozoa than TEST. Sperm DNA stability during incubation at 37°C is a promising, discriminative parameter for selecting semen storage conditions of bulls for elephant AI.

scholarly journals Does the gp130 adenine (A)/thymine (T) (rs1900173) gene polymorphism affect semen quality or sperm DNA damage?

2019 ◽  
Vol 112 (3) ◽  
pp. e374
Author(s):  
Joao Batista A. Oliveira ◽  
Claudia G. Petersen ◽  
Ana Lucia Mauri ◽  
Adriana Renzi ◽  
Laura D. Vagnini ◽  
...  
Keyword(s):  
Dna Damage ◽  
Gene Polymorphism ◽  
Semen Quality ◽  
Sperm Dna Damage ◽  
Sperm Dna

scholarly journals The aging male: Relationship between male age, sperm quality and sperm DNA damage in an unselected population of 3124 men attending the fertility centre for the first time

2019 ◽  
Vol 90 (4) ◽  
pp. 254-259 ◽  
Author(s):  
Alessandro Colasante ◽  
Maria Giulia Minasi ◽  
Filomena Scarselli ◽  
Valentina Casciani ◽  
Vincenzo Zazzaro ◽  
...  
Keyword(s):  
Dna Damage ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Quality ◽  
World Health ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Male Age ◽  
Sperm Dna

Objective: the aim of our study was to put forward insights to treat any possible correlation among sperm quality, sperm DNA damage and male age as they may have fertility implications for men who choose to delay fatherhood. Materials and methods: Our study is a non-interventional retrospective analysis of 3124 semen samples from patients that were investigated for the conventional semen parameters. Tunel test assay was set up for the evaluation of the sperm DNA fragmentation index (DFI). We applied the Kappa index to compare both the 1999 and the 2010 World Health Organization (WHO) reference criteria to evaluate the competence of such semen parameters categorization during the standard routine of our laboratory. Results: With regards to our findings, it is possible to underline a significant relationship between aging and semen volume (p = 0.001), motility (p = 0.009), semen viscosity (p < 0.003) and sperm DNA damage (p < 0.009). We found a trend when focusing on the semen concentration (p = 0.05). The analysis of sperm morphology did not show any influence with advancing age (p = 0.606). When comparing both the 1999 and the 2010 WHO scales we found no accordance in the appraisal of sperm morphology but a very good one in the evaluation of the other parameters. Conclusions: Conventional semen analysis represents the opportunity to draw up a proxy insight on the male fertility status even if semen quality can only indirectly assess the probability of pregnancy. Several studies have verified a decay in the male reproductive system, sperm quality and fertility with advancing age although the reported results are not yet conclusive. Our results substantially agree with those findings outlined in the literature. Moreover we find that the discrepancy between the two WHO reference scales would eventually lead to an improper diagnosis of infertility.

Human Semen Quality, Sperm DNA Damage, and the Level of Reproductive Hormones in Relation to Urinary Concentrations of Parabens

2017 ◽  
Vol 59 (11) ◽  
pp. 1034-1040 ◽  
Author(s):  
Joanna Jurewicz ◽  
Michał Radwan ◽  
Bartosz Wielgomas ◽  
Emila Dziewirska ◽  
Anetta Karwacka ◽  
...  
Keyword(s):  
Dna Damage ◽  
Semen Quality ◽  
Reproductive Hormones ◽  
Human Semen ◽  
Sperm Dna Damage ◽  
Sperm Dna

scholarly journals Are semen quality parameters sufficient for biomonitoring spermatozoa DNA integrity and oxidatively damaged DNA

Biomonitoring ◽  
2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Hueiwang Anna Jeng ◽  
Ruei-Nian Li ◽  
Wen-Yi Lin
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Semen Quality ◽  
Quality Parameters ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Phase System ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna

Abstract:The present study aimed to investigate the relationship between semen quality parameters and DNA integrity, and determine whether semen quality parameters could serve as a reliable biomarker for monitoring sperm DNA damage. Conventional semen parameters from a total of 202 male human subjects were analyzed. DNA fragmentation and 8-oxo-7,8-dihydro-2′- deoxyguanosine (8-oxoGuo) were used to assess sperm DNA integrity. DNA fragmentation was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and sperm chromatin structure assay (SCSA), while 8-oxodGuo was quantified by the liquid chromatography/tandem mass spectrometry (LC-MS/MS) coupled with an on-line solid phase system. The levels of 8-oxodGuo levels in sperm were related to the percentages of DNA fragmentation measured by both the TUNEL and SCSA (r = 0.22, p = 0.048; r = 0.12, p = 0.039). Sperm vitality, motility and morphology from all of the participants exhibited a weak correlation with the levels of 8-oxodGuo and the percentages of DNA fragmentation. Semen quality parameters may be independent of the formation of DNA fragmentation and oxidative adducts in sperm. Semen quality parameters may be insufficient to monitor sperm DNA fragmentation and oxidative damage. DNA damage in sperm is recommended to be included in routine measurements.

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