EFFECTS OF PARSLEY SEEDS AT DIFFERENT LEVELS AS NATURAL ADDITIVES ON SEMEN QUALITY IN RABBIT MALES

2018 ◽  
Vol 1 (1) ◽  
pp. 81-92
Author(s):  
Jamal A. Embark
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Control Diet ◽  
Plasma Concentrations ◽  
Sperm Concentration ◽  
Reproductive Traits ◽  
Quality Parameters ◽  
Control Group ◽  
Semen Collection ◽  
Different Levels

This study is designed to evaluate the effect of using parsley seeds (PAS) at different levels as a natural on semen quality and reduce the effect of lipidperoxidation of bucks. Forty New Zealand rabbit bucks 8 month old were divided into four groups (each 10 rabbit bucks). The groups were divided as follows: Control diet (free from PAS), T1: Control diet + 5g/kg PAS, T2: Control diet + 10g/kg PAS; T3: Control diet + 15g/kg PAS. Semen sample were collected twice a week; from each rabbit buck by artificial vagina. The first semen collection was used to evaluate semen quality parameters. However, the second semen collection was used for analysis semen plasma. Results revealed that feeding diets containing different levels of PAS significantly (P<0.05) increased semen volume, mass activity, individual motility, PSV, sperm concentration, total sperm and seminal plasma activity of ALP enzyme and significant (P<0.05) decrease in dead sperm and seminal plasma concentrations of total lipids, triglycerides, cholesterol and MDA and seminal plasma activities of AST and ALT enzymes and TAC as compared with the control group. In conclusion, dietary supplementation with different levels of PAS, especially at the level of 15 g/kg (T3) resulted in significant improvement in semen quality, antioxidant status and reproductive traits of rabbit bucks.

scholarly journals Effect of Ivermectin on semen parameters and levels of Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) enzymes in seminal plasma of Iraqi Awassi rams

2014 ◽  
Vol 13 (1) ◽  
pp. 115
Author(s):  
Ali. J. Ali
Keyword(s):  
Seminal Plasma ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Semen Quality ◽  
Harmful Effect ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Control Group ◽  
Volume Percentage ◽  
Semen Collection

The objective of this study was to evaluate the effect of Ivermectin on semen quality and estimation of alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in seminal plasma. Six mature Awassi rams aged 2-3 years and weighing 38-42 Kg were used in this study. Semen collection was done weekly for five weeks from the same ram. The first two collection considered as training for the animal and discarded, the second two collection were considers for the control group. After administration of the drug (Ivermectin), semen samples were collected from the Awassi ram 1, 7,14 days. The parameter studies were included semen volume, percentage motility of sperm, sperm concentration, live sperm, and morphology percentage. Samples of seminal plasma were analyzed for the estimation of alanine aminotransferase (ALT), aspartate aminotransferase (AST). Results of this study indicate that Ivermectin leads to significant (P<0.05) decreased in semen parameters after the first semen collection. Reaction time significantly higher (P<0.05) in the treated group after 1 day of injection. Alanine aminotransferase ALT, Aspartate aminotransferase AST show no significant differences in activities in seminal plasma during the study. In conclusion: it is preferable to use the animals for the purpose of reproduction only after at least 7 days after Ivermectin injection due to it harmful effect on semen quality.

scholarly journals Determination of selected components in seminal plasma of donkey stallions and their correlation to semen quality parameters

2019 ◽  
Vol 88 (4) ◽  
pp. 377-384 ◽  
Author(s):  
Michal Vyvial ◽  
Eliška Horáčková ◽  
Markéta Sedlinská ◽  
Eva Jánová ◽  
Šárka Krisová ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Significant Positive Correlation ◽  
Volume Concentration ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Gamma Glutamyl Transferase ◽  
Positive Correlation ◽  
Plasma Selenium ◽  
Glutamyl Transferase

Evaluation of selected components of seminal plasma as markers of ejaculate quality may be of interest when assessing the quality of semen. The aim of the study was to evaluate some biochemical parameters of seminal plasma in donkeys and compare them with the volume, concentration and motility of the ejaculate. A total of 11 ejaculates were collected from five donkey stallions (between one and three from each animal). Each ejaculate was assessed for volume, concentration and motility, and the seminal plasma biochemically examined after centrifugation. Total protein, creatinine, urea, alkaline phosphatase (ALP), alanine-amino-transferase (ALT), aspartate-amino-transferase, creatine kinase, gamma-glutamyl-transferase, lactate-dehydrogenase (LDH), sodium, potassium (K+), calcium, phosphate, chloride, copper (Cu), cholesterol, vitamin E (Vit E), magnesium (Mg2+), seminal plasma selenium (Se SP) and seminal plasma zinc (Zn SP) were identified. Biochemical analysis of the seminal plasma was then statistically evaluated using the Spearman coefficient of correlation. A significant positive correlation between ALP, ALT, LDH, Cu, Se SP and Zn SP and sperm concentration was found. Also there was a significant positive correlation between Vit E and sperm motility. Finally, there was a significant positive correlation between urea, K+, Mg2+ and the ejaculate volume. This is the first report about selected biochemical components of donkey's seminal plasma that we know of. However, further research using a larger number of individuals is necessary to confirm that these components of seminal plasma are useful as markers of ejaculate quality in donkey stallions.

Effect of dietary ellagic acid supplementation on semen quality parameters in chickens

2015 ◽  
Vol 55 (1) ◽  
pp. 107 ◽  
Author(s):  
M. Shanmugam ◽  
S. V. Rama Rao
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Seminal Plasma ◽  
Ellagic Acid ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Superoxide Dismutase Activity ◽  
Semen Parameters ◽  
Reducing Ability

Ellagic acid (EA), a polyphenoloic constituent in fruits and nuts, has been shown to have antioxidant and antiapoptotic properties. The aim of this study was to investigate the effect of dietary EA supplementation on semen quality parameters in chickens. Forty roosters (Dahlem Red breed), 28 weeks of age, were randomly assigned to four groups and fed diets containing EA at 0, 25, 50, or 75 mg/kg for 8 weeks. Semen was evaluated for volume, appearance, individual motility, sperm concentration, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) dye reduction test, and live, dead and abnormal sperm counts. The seminal plasma was separated and evaluated for protein, alkaline phosphatase, and lipid peroxidation. At the end of the experiment, blood from individual birds was collected, and serum was separated and evaluated for superoxide dismutase activity, lipid peroxidation, and ferric-reducing ability of plasma. Supplementation with EA had no effect (P > 0.05) on gross semen parameters. Seminal plasma protein and lipid peroxidation were different (P < 0.01) between the treatment groups. The group fed EA at 75 mg/kg had high (P < 0.01) seminal plasma lipid peroxidation and lower (P < 0.05) serum superoxide dismutase activity. In conclusion, dietary supplementation of EA in chickens had no effect on the gross semen qualities and increased lipid peroxidation in seminal plasma.

scholarly journals Variation of Semen Quality Among Three Generations (F1, F2 and F3) of Holstein Friesian Upgraded Breeding Bulls of Bangladesh

2020 ◽  
Vol 7 (3) ◽  
pp. 457-463
Author(s):  
Faruk Hossain ◽  
Md Golam Sorowar ◽  
Sharmin Akter Suma ◽  
Abdullah Al Mansur ◽  
Md Mahbubul Hoque ◽  
...  
Keyword(s):  
First Generation ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Quality Parameter ◽  
Microsoft Excel ◽  
Three Generations ◽  
Holstein Friesian ◽  
Recorded Data ◽  
Mass Activity

The experiment was conducted in a well-known private livestock research and development farm in Bangladesh to find out the variation of semen quality parameter among three generations (F1, F2 and F3) of Holstein Friesian upgraded breeding bulls. A total of 312 ejaculates were collected from 6 upgraded breeding bullsthrought the experimental year. The recorded data were summarized using Microsoft Excel 2010 and analyzed using GraphPad Prism 5 softwere. Out of the 312 ejaculates, 273 (87.50%) were found to be creamy in color followed by 26 (8.33%) and 13 (4.17%) as yellowish and watery, respectively. Generation had significant (P<0.05) effect on ejaculate volume, consistancy, mass activity, sperm concentration, initial and post freezing motility. The highest (7.389±0.19ml) and the lowest (5.156±0.13ml) volume of semen were found in third (F3) and first generation (F1), respectively. The mass activity ranges from 3.74±0.04 to 4.30±0.05. Sperm concentration and pH varied insignificantly (p>0.05) but initial motility and post freezing motility had the significant differences among the three generations. Initial motility ranges from 75.87±0.32 to 78.40±0.38 percent and the post freezing motility ranges from 50.38±0.41 to 52.16±0.43 percent. It could be concluded that most of the semen quality parameters were influenced by generation and freezing. Semen characteristics were better in F2 followed by F3 and F1 generation in upgraded Holstein Friesian bulls. Res. Agric., Livest. Fish.7(3): 457-463,  December 2020

scholarly journals Effect of Age and Frequency of Collection on Quality of Jersey Bulls Semen at National Livestock Breeding Center (NLBC), Nepal

2019 ◽  
Vol 7 (1) ◽  
pp. 88-95 ◽  
Author(s):  
S. Sankhi ◽  
K.R. Sapkota ◽  
B. Regmi
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Bull Semen ◽  
Livestock Breeding ◽  
Abnormal Sperm ◽  
Breeding Center ◽  
Live Sperm ◽  
Level Of Significance ◽  
Effect Of Age

Objective: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara. Materials and Methodology: Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0 Results: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection. Conclusion: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95

scholarly journals Association between semen quality among men with different occupational exposures and risk of recurrent spontaneous abortion in island residents

2019 ◽  
Vol 02 (02) ◽  
pp. 49-57
Author(s):  
Wenli Cao ◽  
Xinyao Pan ◽  
Feijun Ye ◽  
Jing Zhou ◽  
Zengshu Huang ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Occupational Exposures ◽  
Recurrent Spontaneous Abortion ◽  
Semen Parameters ◽  
Control Group ◽  
Effective Prevention ◽  
Mechanistic Investigation ◽  
Significant Difference

Objective: This study aimed to evaluate the semen quality of male adults whose spouses suffer from recurrent spontaneous abortion (RSA) in the island area, and to explore the association between RSA and occupational exposures. Methods: A total of 131 male patients were recruited and divided into two groups: spouse with recurrent spontaneous abortion group (RSA group, [Formula: see text]) and the normal fertility group (control group, [Formula: see text]). Information such as height, weight and occupational exposure history of 131 men were obtained. Semen samples were collected and analyzed. Differences in semen parameters and DNA fragmentation index (DFI) between the two groups were compared. Odds ratios (ORs) and their corresponding 95% confidence intervals were calculated to evaluate the association between occupational exposures and RSA status. Results: Overall, no significant difference was found in sperm concentration, progressive motility and normal morphology rate between RSA and control groups. Only DFI was observed to be significantly higher in the RSA group. Evaluation of the receiver operating characteristic (ROC) curve showed DFI (AUC: 0.623, [Formula: see text]) could discriminate between males from the couples with RSA and without RSA. Conclusion: The analysis of conventional semen parameters could not directly reflect their influence on embryonic development, and test of integrity of the sperm DNA is of paramount importance to fully understand male fertility. It is crucial to conduct studies regarding occupational exposures and pregnancy loss and/or RSA risk, since it will provide population-level data to aid in the identification of important risk factors that warrant further mechanistic investigation, and eventually lead to effective prevention and treatment strategies.

104 COMPARING DIFFERENT LEVELS OF OSMOLALITY OF SUCROSE EXTENDER ON THE VIABILITY OF SPERMATOZOA IN BACTRIAN CAMEL (CAMELUS BACTRIANUS)

2006 ◽  
Vol 18 (2) ◽  
pp. 160 ◽  
Author(s):  
S. Mosaferi ◽  
A. Niasari-Naslaji ◽  
N. Bahmani ◽  
A. A. Gharahdaghi ◽  
A. Abarghani ◽  
...  
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Bactrian Camel ◽  
Dromedary Camel ◽  
Camelus Bactrianus ◽  
Semen Collection ◽  
History Of ◽  
Different Levels ◽  
Artificial Vagina

Disaccharides have been used as an extender for dromedary camel semen (Bravo et al. 2000 Anim. Reprod. Sci. 62, 173-193). More recently we have investigated the effect of different concentrations of lactose extender on the viability of Bactrian camel spermatozoa (Mosafer et al. 2005 Reprod. Fertil. Dev. 17, 197). Considering the osmolality (316.1 � 1.48 mOsm/kg) and pH (7.4 � 0.03) of Bactrian camel semen (Mosaferi et al. 2005 Theriogenology 63, 92-101), the objective of this study was to investigate the effect of osmolality of sucrose extender on the viability of Bactrian camel spermatozoa. Sucrose at the concentrations of 9, 10, 11, 12, and 13% with osmolalities of 292, 331, 356, 386, and 410 mOsm/kg, respectively, were prepared. All extenders contained 20% egg yolk and antibiotics, with pH adjusted to 6.9. Semen was collected from camels with a sound history of semen quality and fertility (n = 3) using a modified artificial vagina and divided into different treatments after mechanical reduction of semen viscosity (3). Progressive forward motility of spermatozoa was examined at the time of semen collection and at 4, 12, and 24 h after incubation at 4�C. Data were analyzed using the GLM procedure in SAS/STAT after arcsin transformation. At the time of semen dilution, the progressive forward motility of spermatozoa was greater at osmolality of 331 (23%) compared with 292 (1%), 386 (6%), and 410 (3.5%) mOsm/kg (P < 0.05). No progressive forward motility of spermatozoa was noticed after 4 h incubation at 4�C at osmolalities of 292, 386, and 410 mOsm/kg. At this time, a significant decrease (P < 0.05) of progressive forward motility occurred at osmolalities of 331 (4%) and 356 (0.5%) compared with that of the time of dilution. After 12 and 24 h incubation at 4�C, no progressive forward motility of spermatozoa was detected in any of these extenders. In conclusion, 10% sucrose (331 mOsm/kg) at the adjusted pH of 6.9 was the most suitable concentration of this disaccharide for preserving Bactrian camel semen for less than 4 h under chilled conditions.

Relationship between semen quality and seminal plasma cholesterol, triacylglycerols and proteins in the domestic cat

2019 ◽  
Vol 22 (10) ◽  
pp. 882-889 ◽  
Author(s):  
María F García ◽  
Romina Nuñez Favre ◽  
María C Stornelli ◽  
Ramiro Rearte ◽  
María C García Mitacek ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Count ◽  
Sodium Dodecyl ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Domestic Cat ◽  
Sds Page ◽  
The Relationship

Objectives The current study aimed to evaluate the relationship between specific seminal plasma components – cholesterol (CHOL), triacylglycerols (TAG) and total protein (PROT) concentrations – and semen quality in cats. A further aim was to determine the relationship between specific seminal protein bands and semen quality. Methods Thirteen toms, 2–5 years of age, were included. Semen collection was performed by electroejaculation every 4 weeks. Fifty-eight ejaculates were assessed for motility, velocity, volume, sperm concentration, total sperm count, viability, acrosome integrity, plasma membrane integrity and sperm morphology. Samples were divided into two groups: good semen quality (GSQ) and poor semen quality (PSQ). After evaluation, seminal plasma was separated from the sperm by centrifugation and stored at −20°C. CHOL, TAG and PROT concentrations were then assessed and seminal plasma protein profile was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results Seminal plasma CHOL and TAG concentrations, motility, velocity, sperm concentration, total sperm count and sperm morphology were significantly higher in GSQ cats compared with PSQ cats ( P <0.01). Moreover, seminal plasma SDS-PAGE analysis showed an identifiable extra band exclusively in the GSQ group. Conclusions and relevance Data obtained in this study showed that seminal plasma CHOL and TAG concentrations and specific protein bands could be used to improve semen evaluation in toms. In this sense, the 14 kDa protein band could be a valuable marker for semen quality in the cat and should be further investigated. However, more studies are necessary to determine its relationship with fertility.

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