Effects of contaminated surface water and groundwater from a rare earth mining area on the biology and the physiology of Sprague-Dawley rats

2021 ◽  
Vol 761 ◽  
pp. 144123
Author(s):  
Xiaoying He ◽  
Ting Yuan ◽  
Xinying Jiang ◽  
Hui Yang ◽  
Chun Li Zheng
Keyword(s):  
Rare Earth ◽  
Surface Water ◽  
Mining Area ◽  
Sprague Dawley ◽  
Surface Water And Groundwater ◽  
Sprague Dawley Rats

scholarly journals Surface Water and Groundwater Quality Evaluation in a Mining Area

2020 ◽  
Vol 71 (6) ◽  
pp. 124-131
Author(s):  
Alina Cochiorca ◽  
Valentin Nedeff ◽  
Narcis Barsan ◽  
Ion Sandu ◽  
Emilian Mosnegutu ◽  
...  
Keyword(s):  
Surface Water ◽  
Dissolved Oxygen ◽  
Mining Area ◽  
Chemical Parameters ◽  
Chemical Indicators ◽  
Surface Water And Groundwater ◽  
Physico Chemical ◽  
Three Rivers

This paper presents a monitoring of groundwater and surface water in the mining area Tg. Ocna, by analyzing 16 physico-chemical parameters (pH, turbidity, dissolved oxygen, Pb, Ni, Cu, Fe, Mn, Zn, Cr, Mg, SO42-, Al, NO2-, NO3-, NH4+), from three rivers Slanic, Trotus and Valcele, a small lake Groapa Burlacu, a fountain and three monitoring drilling wells (F11, F16 and F17). Studies on the quality of surface water and groundwater in areas near mines are important due to extraction and exploitation of salt. The samples were collected in April 2019. Following the analyzes, their results are included in the following concentration ranges: pH 7.21 to 8.46 unit. pH, turbidity 0.54 to 169 NTU, dissolved oxygen 6 to 8.59 mg/L, Pb 0.075 to 0.095 mg/L, Ni 0.026 to 1.05 mg/L, Cu 0.088 to 0.809 mg/L, NO2- 0.001 to 0.037 mg/L and NO3- 0.290 to 4.34 mg/L. The pH, turbidity and dissolved oxygen were measured in situ using portable equipment�s. As for the other parameters, they were analyzed in the water laboratory from Vasile Alecsandri University of Bacau by using spectrophotometry. Some concentrations of the physico-chemical indicators of the water samples analyzed were found to be more than the water quality classes.

Reversed-Phase High-Performance Liquid Chromatography for the Determination of 15 Rare Earth Elements in Surface Water Sample Collected in a Mining Area from Lavras do Sul/RS, Brazil

2019 ◽  
Vol 82 (5) ◽  
pp. 843-856 ◽  
Author(s):  
Alexsandro Nunes Colim ◽  
Paulo Cícero do Nascimento ◽  
Bruna Avila Wiethan ◽  
Franciéle Rovasi Adolfo ◽  
Lucielle Codeim Dresch ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Rare Earth ◽  
Surface Water ◽  
Rare Earth Elements ◽  
High Performance ◽  
Mining Area ◽  
Reversed Phase ◽  
Surface Water Sample

Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

1982 ◽  
Vol 40 ◽  
pp. 216-217
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Microscopic Study ◽  
Pituitary Adenomas ◽  
Wistar Rats ◽  
Microscopic Level ◽  
Sprague Dawley ◽  
Prolactin Cells ◽  
Light Microscopic Level ◽  
Ultrastructural Investigation ◽  
Sprague Dawley Rats ◽  
Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

Early Development of Drug-Induced Hepatic Necrosis

1971 ◽  
Vol 29 ◽  
pp. 576-577
Author(s):  
F. G. Zaki ◽  
E. Detzi ◽  
C. H. Keysser
Keyword(s):  
Early Development ◽  
Hepatic Necrosis ◽  
Screening Tests ◽  
Dense Matrix ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Drug Induced ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
Microscopic Studies

This study represents the first in a series of investigations carried out to elucidate the mechanism(s) of early hepatocellular damage induced by drugs and other related compounds. During screening tests of CNS-active compounds in rats, it has been found that daily oral administration of one of these compounds at a dose level of 40 mg. per kg. of body weight induced diffuse massive hepatic necrosis within 7 weeks in Charles River Sprague Dawley rats of both sexes. Partial hepatectomy enhanced the development of this peculiar type of necrosis (3 weeks instead of 7) while treatment with phenobarbital prior to the administration of the drug delayed the appearance of necrosis but did not reduce its severity.Electron microscopic studies revealed that early development of this liver injury (2 days after the administration of the drug) appeared in the form of small dark osmiophilic vesicles located around the bile canaliculi of all hepatocytes (Fig. 1). These structures differed from the regular microbodies or the pericanalicular multivesicular bodies. They first appeared regularly rounded with electron dense matrix bound with a single membrane. After one week on the drug, these vesicles appeared vacuolated and resembled autophagosomes which soon developed whorls of concentric lamellae or cisterns characteristic of lysosomes (Fig. 2). These lysosomes were found, later on, scattered all over the hepatocytes.

Ultrastructural investigation of spontaneous pituitary gonadotroph cell adenomas in aging Sprague-Dawley rats

1983 ◽  
Vol 41 ◽  
pp. 702-703
Author(s):  
D. J. McComb ◽  
J. Beri ◽  
F. Zak ◽  
K. Kovacs
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Epoxy Resin ◽  
Immunoelectron Microscopy ◽  
Tumor Type ◽  
Gonadal Function ◽  
Sprague Dawley ◽  
Male And Female ◽  
Reticulin Fibers ◽  
Sprague Dawley Rats

Gonadotroph cell adenomas of the pituitary are infrequent in human patients and are not invariably associated with altered gonadal function. To date, no animal model of this tumor type exists. Herein, we describe spontaneous gonadotroph cell adenomas in old male and female Sprague-Dawley rats by histology, immunocytology and electron microscopy.The material consisted of the pituitaries of 27 male and 38 female Sprague Dawley rats, all 26 months of age or older, removed at routine autopsy. Sections of formal in-fixed, paraffin-embedded tissue were stained with hematoxylin-phloxine-saffron (HPS), the PAS method and the Gordon-Sweet technique for the demonstration of reticulin fibers. For immunostaining, sections were exposed to anti-rat β-LH, anti-ratβ-TSH, anti-rat PRL, anti-rat GH and anti-rat ACTH 1-39. For electron microscopy, tissue was fixed in 2.5% glutaraldehyde, postfixed in 1% OsO4 and embedded in epoxy-resin. Tissue fixed in 10% formalin, embedded in epoxy resin without osmification, was used for immunoelectron microscopy.

Freeze-etch studies of epiphyseal growth plate cartilage reveal matrix vesicles associated with calcification

1978 ◽  
Vol 36 (2) ◽  
pp. 670-671
Author(s):  
Russell N. A. Cecil ◽  
H. Clarke Anderson
Keyword(s):  
Chromic Acid ◽  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Matrix Vesicle ◽  
Glycerol Solution ◽  
Sprague Dawley ◽  
Epiphyseal Growth Plate ◽  
Growth Plates ◽  
Sprague Dawley Rats ◽  
Tibial Epiphyseal

Unfixed proximal tibial epiphyseal growth plates were studied by freeze-etch to confirm the presence of extracellular calcifying matrix vesicles and to determine the substructure of matrix vesicle membranes as compared to plasma and other membranes of intact chondrocytes. Growth plates from 6-10 week old Sprague-Dawley rats were cut into 1x3 mm blocks whose long dimension was oriented either perpendicular or parallel to the long axis of the tibia. Some blocks were fixed at pH 7. 0 in 0. 2M cacodylate - buffered 2. 5% glutaraldehyde for 1 hour at 4ÅC. The blocks were immersed in 30% glycerol solution at 4ÅC for 1 hour, frozen in liquid nitrogen, and then fractured, etched for 2 minutes, and coated with platinum, carbon and 0. 2% Formvar solution. The replicas were cleaned with chromic acid, floated onto Formvar coated grids, and examined with a Phillips EM 300 electron microscope.Fixed and unfixed specimens appeared similar in ultrastructure. Chondrocytes, matrix, and matrix vesicles were identified. In specimens fractured parallel to the long axis of the tibia, the reserve, proliferative, hypertrophic, and calcifying zones could be discerned as described by light and electron microscopy.

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