Selenate regulates the activity of cell wall enzymes to influence cell wall component concentration and thereby affects the uptake and translocation of Cd in the roots of Brassica rapa L

2022 ◽  
pp. 153156
Author(s):  
Li Yang ◽  
ShuangQin Huang ◽  
Yang Liu ◽  
ShunAn Zheng ◽  
Hong Liu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Brassica Rapa ◽  
Component Concentration ◽  
Cell Wall Component ◽  
Cell Wall Enzymes

Molecular Structure of the Outermost Cell Wall Component of Spirillum Serpens

1977 ◽  
Vol 35 ◽  
pp. 342-343
Author(s):  
Wah Chiu ◽  
David Grano
Keyword(s):  
Molecular Structure ◽  
Cell Wall ◽  
Outer Membrane ◽  
Gel Electrophoresis ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Cell Wall Component ◽  
Protein Components ◽  
Exposure Technique ◽  
Structural Aspects

The periodic structure external to the outer membrane of Spirillum serpens VHA has been isolated by similar procedures to those used by Buckmire and Murray (1). From SDS gel electrophoresis, we have found that the isolated fragments contain several protein components, and that the crystalline structure is composed of a glycoprotein component with a molecular weight of ∽ 140,000 daltons (2). Under an electron microscopic examination, we have visualized the hexagonally-packed glycoprotein subunits, as well as the bilayer profile of the outer membrane. In this paper, we will discuss some structural aspects of the crystalline glycoproteins, based on computer-reconstructed images of the external cell wall fragments.The specimens were prepared for electron microscopy in two ways: negatively stained with 1% PTA, and maintained in a frozen-hydrated state (3). The micrographs were taken with a JEM-100B electron microscope with a field emission gun. The minimum exposure technique was essential for imaging the frozen- hydrated specimens.

scholarly journals Synthesis of Lipid-Carbohydrate-Peptidyl-RNA Conjugates to Explore the Limits Imposed by the Substrate Specificity of Cell Wall Enzymes on the Acquisition of Drug Resistance

2018 ◽  
Vol 24 (56) ◽  
pp. 14911-14915 ◽  
Author(s):  
Matthieu Fonvielle ◽  
Ahmed Bouhss ◽  
Coralie Hoareau ◽  
Delphine Patin ◽  
Dominique Mengin-Lecreulx ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Cell Wall ◽  
Substrate Specificity ◽  
Cell Wall Enzymes

Kinetic study on the liquefaction of wood and its three cell wall component in polyhydric alcohols

2014 ◽  
Vol 113 ◽  
pp. 1596-1600 ◽  
Author(s):  
Hairong Zhang ◽  
Huijuan Yang ◽  
Haijun Guo ◽  
Chao Huang ◽  
Lian Xiong ◽  
...  
Keyword(s):  
Cell Wall ◽  
Kinetic Study ◽  
Cell Wall Component ◽  
Polyhydric Alcohols

scholarly journals Contribution of the Cell Wall Component Teichuronopeptide to pH Homeostasis and Alkaliphily in the Alkaliphile Bacillus lentus C-125

1999 ◽  
Vol 181 (21) ◽  
pp. 6600-6606 ◽  
Author(s):  
Rikizo Aono ◽  
Masahiro Ito ◽  
Takayoshi Machida
Keyword(s):  
Cell Wall ◽  
Amino Acid Residues ◽  
Alkaline Ph ◽  
Structural Components ◽  
Chromosomal Dna ◽  
Ph Homeostasis ◽  
Cell Wall Component ◽  
Bacillus Lentus ◽  
Defective Mutant ◽  
Alkaline Conditions

ABSTRACT A teichuronopeptide (TUP) is one of major structural components of the cell wall of the facultative alkaliphilic strain Bacillus lentus C-125. A mutant defective in TUP synthesis grows slowly at alkaline pH. An upper limit of pH for growth of the mutant was 10.4, while that of the parental strain C-125 was 10.8. GenetupA, directing synthesis of TUP, was cloned from C-125 chromosomal DNA. The primary translation product of this gene is likely a cytoplasmic protein (57.3 kDa) consisting of 489 amino acid residues. Introduction of the tupA gene into the TUP-defective mutant complemented the mutation responsible for the pleiotropic phenotypes of the mutant, leading to simultaneous disappearance of the defect in TUP synthesis, the diminished ability for cytoplasmic pH homeostasis, and the low tolerance for alkaline conditions. These results demonstrate that the acidic polymer TUP in the cell wall plays a role in pH homeostasis in this alkaliphile.

scholarly journals Calcium- and hormone-driven regulation of secondary metabolism and cell wall enzymes in grape berry cells

2018 ◽  
Vol 231 ◽  
pp. 57-67 ◽  
Author(s):  
Viviana Martins ◽  
Ana Garcia ◽  
Cátia Costa ◽  
Mariana Sottomayor ◽  
Hernâni Gerós
Keyword(s):  
Cell Wall ◽  
Secondary Metabolism ◽  
Grape Berry ◽  
Cell Wall Enzymes
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