Comparative pharmacokinetics using a microbiological assay and high performance liquid chromatography following intravenous administration of cefquinome in lactating goats with and without experimentally induced Staphylococcus aureus mastitis

2015 ◽  
Vol 133 ◽  
pp. 67-76 ◽  
Author(s):  
S.A. El Badawy ◽  
A.M. Amer ◽  
G.M. Kamel ◽  
K.M. Eldeib ◽  
P.D. Constable
Keyword(s):  
Staphylococcus Aureus ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Intravenous Administration ◽  
High Performance ◽  
Microbiological Assay ◽  
Lactating Goats ◽  
Experimentally Induced

scholarly journals Correlations between milk production and kinetic variables in milk of cephalothin administered to lactating goats

2012 ◽  
Vol 49 (No. 10) ◽  
pp. 370-372 ◽  
Author(s):  
R. Rule ◽  
C. Cordiviola ◽  
M. Vita ◽  
R. Lacchini
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Milk Production ◽  
Standard Error ◽  
High Performance ◽  
Correlation Coefficients ◽  
Intravenous Route ◽  
Milk Samples ◽  
Lactating Goats

The aim of the present study was to correlate the milk production and the kinetic variables in milk of cephalothin administered to goats. Twenty healthy creole goats in milk production were used. Cephalothin was administered by intravenous route (20 mg/kg b.w.). Milk samples were collected at 0.25, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 8.0 and 12.0 hours postadministration of the antibiotic. Cephalothin concentrations were measured in milk samples by high performance liquid chromatography. The values (mean &plusmn; standard error) of milk production collected during 24 hours previous to the administration of the antibiotic were 761.5 &plusmn; 111.1 ml. The results of the kinetic variables (mean &plusmn; standard error) of cephalothin in milk were: AUC = 5.4 &plusmn; 1.6 &micro;g/ml/h; C<sub>max</sub>= 1.1 &plusmn; 0.3 &micro;g/ml and t<sub>max </sub>= 1.7 &plusmn; 0.1 h. The correlation coefficients AUC-milk production, C<sub>max</sub>-milk production and t<sub>max</sub>-milk production were: 0.602 (P &lt; 0.01), 0.596 (P &lt; 0.01) and 0.398 (P &lt; 0.1), respectively. In conclusion, the areas under the curve and the maximum concentrations and the time to reach them in milk are in fact related to the volume of milk produced by the goats

scholarly journals A Spectrum of Changes Occurs in Peptidoglycan Composition of Glycopeptide-Intermediate Clinical Staphylococcus aureus Isolates

2001 ◽  
Vol 45 (1) ◽  
pp. 280-287 ◽  
Author(s):  
Susan Boyle-Vavra ◽  
Harald Labischinski ◽  
Christine C. Ebert ◽  
Kerstin Ehlert ◽  
Robert S. Daum
Keyword(s):  
Staphylococcus Aureus ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Biochemical Change ◽  
Glycopeptide Resistance ◽  
Resistance Phenotype ◽  
Consistent Feature ◽  
The U.S

ABSTRACT The mechanism of glycopeptide resistance in Staphylococcus aureus is not known with certainty. Because the target of vancomycin is the d-Ala–d-Ala terminus of the stem peptide of the peptidoglycan precursor, by subjecting muropeptides to reversed-phase high-performance liquid chromatography, we investigated peptidoglycan obtained from glycopeptide-intermediateS. aureus (GISA) isolates for changes in composition and evaluated whether any peptidoglycan structural change was a consistent feature of clinical GISA isolates. GISA isolates Mu50 and Mu3 from Japan had the large glutamate-containing monomeric peak demonstrated previously, although strain H1, a vancomycin-susceptible MRSA isolate from Japan that was clonally related to Mu3 and Mu50, and afemC mutant that we studied, did also. For the U.S. GISA isolates, strain NJ had a large monomeric peak with a retention time identical to that described for the glutamate-containing monomer in strains H1, Mu3, and Mu50. However, a much smaller corresponding peak was seen in GISA MI, and this peak was absent from both GISA PC and a recent GISA isolate obtained from an adult patient in Illinois (strain IL). These data suggest that a uniform alteration in peptidoglycan composition cannot be discerned among the GISA isolates and indicate that a single genetic or biochemical change is unlikely to account for the glycopeptide resistance phenotype in the clinical GISA isolates observed to date. Furthermore, a large monomeric glutamate-containing peak is not sufficient to confer the resistance phenotype.

scholarly journals Senyawa Antibakteri Escherichia coli ATCC 35218 dan Staphylococcus aureus ATCC 25923 dari Kapang Endofit Taman Nasional Gunung Halimun

2012 ◽  
Vol 12 (1) ◽  
pp. 21 ◽  
Author(s):  
Ruth Melliawati ◽  
Harni Harni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Retention Time ◽  
Endophytic Fungus ◽  
High Performance ◽  
Agar Plate ◽  
Chloroform Fraction ◽  
Antibacterial Compounds

Endophytic fungus is a microorganism which live in the interstitial spaces healthy tissues of the host plant, andhas capability to produce secondary metabolite such as micotoxin, antibiotic and antiviral. This research wasaimed to find out the isolates of endophytic fungus produce antibacterial compounds to inhibit Escerichia coliATCC 35218 and Staphylococcus aureus ATCC 25923, and to investigate the Retention time (Rt) of the antibacterialcompounds produced by endophytic fungus with High Performance Liquid Chromatography (HPLC) methods.Diffusion Agar Plate Method was used to examine the antibacterial compounds on Escerichia coli ATCC 35218 andStaphylococcus aureus ATCC 25923. While the antibacterial compounds were examined with Thin LayerChromatography (TLC) and High Performance Liquid Chromatography (HPLC) methods, and the result werecompared with Chloramphenicol and Ampicillin antibiotic standart. Two isolates of endophytic fungus namelyHl.46F.211 and HL.57F.258 were inhibited the growth Escherichia coli ATCC 35218 and three isolates namelyHL.48F217, HL.53F.243 and HL.57F.258 showed antagonistic action against Staphylococcus aureus ATCC 25923.The results of TLC and HPLC analysing method show that antibacterial compounds produced by endophytic fungusHL.46F.211 had Rt (Retention Time) rate similar with antibiotic Chloramphenicol at 2,796 (at water fraction) and Rtantibiotic Amphycillin at 2,662 (at Chloroform fraction). While antibacterial compounds produced by endophyticfungus HL.57F.258 had Rt rate similar with antibiotic Amphycillin at 2,650 (at Chloroform fraction).

scholarly journals EVALUASI FORMULASI RADIOFARMAKA 99mTc-SIPROFLOKSASIN DENGAN REDUKTOR SnCl2·2H2O dan Sn-TARTRAT

2019 ◽  
Vol 20 (2) ◽  
pp. 57
Author(s):  
Rizky Juwita Sugiharti ◽  
Iim Halimah ◽  
Maula Eka Sriyani ◽  
Eva Maria Widyasari
Keyword(s):  
Staphylococcus Aureus ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance

99mTc-siprofloksasin adalah radiofarmaka berbasis antibiotik untuk diagnosis infeksi yang disebabkan oleh bakteri. Pada penelitian ini radiofarmaka 99mTc-siprofloksasin disiapkan dengan menggunakan dua macam reduktor yaitu SnCl2·2H2O dan Sn-tartrat. Penelitian ini bertujuan membandingkan masing-masing reduktor untuk menghasilkan radiofarmaka 99mTc-siprofloksasin dengan nilai kemurnian radiokimia yang tinggi dan akumulasi yang baik di daerah infeksi. Hasil dari penelitian ini menunjukkan bahwa kondisi optimum diperoleh pada jumlah reduktor SnCl2·2H2O sebanyak 25 µg dengan kemurnian radiokimia sebesar  94,25 + 2,04 dan Sn-tartrat sebanyak 400 µg dengan kemurnian radiokimia sebesar  95,06 + 1,00 Pengujian kemurnian  radiokimia 99mTc-siprofloksasin menggunakan High Performance Liquid Chromatography memperlihatkan profil kromatogram yang sama untuk masing-masing reduktor dengan puncak dari 99mTc-siprofloksasin berada pada waktu retensi 7,17 menit. Hasil uji biodistribusi pada paha mencit yang terinfeksi bakteri Staphylococcus aureus untuk formula 99mTc-siprofloksasin dengan reduktor SnCl2·2H2O 1 jam dan 3 jam pasca injeksi menunjukkan rasio target/nontarget relatif konstan yaitu sebesar 2,71 dan 2,25. Sedangkan rasio target/nontarget 99mTc-siprofloksasin dengan reduktor Sn-tartrat pada 1 jam dan 3 jam pasca injeksi sebesar 1,78 dan 2,20 Dari hasil penelitian dapat diketahui bahwa formulasi radiofarmaka 99mTc-siprofoksasin dengan dua macam reduktor SnCl2.2H2O dan Sn – tartrat memiliki karakteristik yang berbeda. Kata kunci : radiofarmaka,99mTc-siprofloksasin, reduktor, kemurnian radiokimia

Quality of Vancomycin for Injection Formulations in Brazil

2019 ◽  
Vol 15 (3) ◽  
pp. 280-285
Author(s):  
Gabriela Secco ◽  
Cristiane Sachetti ◽  
Luciana Grazziotin Rossato-Grando ◽  
Siomara Regina Hahn ◽  
Lidiane Riva Pagnussat ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Generic Drug ◽  
Health Professionals ◽  
High Performance ◽  
Hospital Costs ◽  
Microbiological Assay ◽  
Hospital Environment

Background: The presence of impurities in vancomycin compromised the safety and contributed to decrease of its use for years. In Brazil, vancomycin generic drug represents an option to reduce hospital costs. However, the controversy over the quality of these formulations and their relationship to effectiveness and safety raised concerns. Objective and Methods: To assess in vitro quality of vancomycin injections through uniformity of weight, pH, clarity of solution, microbiological assay and impurities determination by High Performance Liquid Chromatography (HPLC). Results: The samples were approved in the tests. Conclusion: The injectable formulations of vancomycin proved to be safe for use in hospital environment. This work contributes to increase health professionals’ confidence on generic vancomycin.

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