scholarly journals A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine

2016 ◽  
Vol 232 ◽  
pp. 257-263 ◽  
Author(s):  
Kun Yin ◽  
Yixuan Wu ◽  
Shasha Wang ◽  
Lingxin Chen
Keyword(s):  
Copper Ion ◽  
Recognition Element ◽  
Fluorescent Biosensor ◽  
Biological Recognition

Modular Peptide-Based Hybrid Nanoprobes for Bio-Imaging and Bio-Sensing

2014 ◽  
Vol 1621 ◽  
pp. 155-161 ◽  
Author(s):  
Banu Taktak Karaca ◽  
James Meyer ◽  
Sarah VanOosten ◽  
Mark Richter ◽  
Candan Tamerler
Keyword(s):  
Self Assembly ◽  
Copper Ions ◽  
Copper Ion ◽  
Gold Surface ◽  
Protein Arrays ◽  
Recognition Element ◽  
Ion Sensing ◽  
Wide Range ◽  
Biological Recognition ◽  
Assembly Technique

ABSTRACTThe self-organization of functional proteins directly onto solid materials is attractive to a wide range of biomaterials and systems that need to accommodate a biological recognition element. In such systems, inorganic binding peptides may be an essential component due to their high affinity and selective binding features onto different types of solid surfaces. This study demonstrates a peptide-enabled self-assembly technique for designing well-defined protein arrays over a metal surface. To illustrate this concept, we designed a fusion protein that simultaneously displays a red fluorescence protein (DsRed-monomer), which is highly selective for copper ions, and a gold binding peptide AuBP. The peptide tag, AuBP, self-directs the organization of DsRed-monomer protein onto a gold surface and forms arrays built upon an efficient control of the organic/inorganic interface at the molecular level. The peptide-assisted design offers a modular approach for fabrication of fluorescent-based protein arrays with copper ion sensing ability.

scholarly journals Effect of Lactamase Inhibitors on the Biosensor Penp during the Measurement of Lactam Antibiotics Concentration

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1237
Author(s):  
Dagoberto Soto ◽  
Camila Silva ◽  
Cristian Ugalde ◽  
Kwok-Yin Wong ◽  
Yun-Chung Leung ◽  
...  
Keyword(s):  
Resistance Mechanism ◽  
Common Element ◽  
Clinical Samples ◽  
Fluorescent Biosensor ◽  
Biological Recognition ◽  
Common Strategy ◽  
Lactam Ring ◽  
Mechanism Of Interaction ◽  
Fluorescence Level ◽  
Covalently Linked

PenP is a fluorescent biosensor of lactam antibiotics (LA). It is structurally derived from the mutant lactamase TEM-1 comprising the substitution E166C, where fluorescein is covalently linked to cysteine. The presence of LA in the medium produces a change in the intrinsic fluorescence level of the biosensor, and the integral of the fluorescence level over time correlates directly with the LA concentration. Previously, we have successfully used PenP to determine the concentration of lactam antibiotics in clinical samples. The use of lactamase inhibitors (LI) is a common strategy to enhance the effect of LA due to the inhibition of an important resistance mechanism of pathogenic microorganisms. Structurally, LI and LA share the common element of recognition of lactamases (the lactam ring), but they differ in the reversibility of the mechanism of interaction with said enzyme. Because the biological recognition domain of PenP is derived from a lactamase, LI is expected to interfere with the PenP detection capabilities. Surprisingly, this work provides evidence that the effect of LI is marginal in the determination of LA concentration mediated by PenP.

scholarly journals Development of membrane in microfluidic device for sorting and detection for potential heterogeneity investigation

2021 ◽  
Author(s):  
Dehi Joung
Keyword(s):  
Pore Size ◽  
Mechanical Stability ◽  
Membrane Separation ◽  
Cross Flow ◽  
Polymeric Materials ◽  
Membrane Properties ◽  
Glycol Diacrylate ◽  
Recognition Element ◽  
Size Dependent ◽  
Biological Recognition

Membrane fabrication and integration with microfluidic devices has received increased attention for applications including bio-detection (a device providing analytical information in a selective and quantitative manner using a biological recognition element), membrane-based separation, and biological sample purification. The main challenges associated with these applications have been: 1) meeting sensitivity/selectivity requirements, 2) decreasing costs, 3) maintaining the mechanical stability of the membrane, 4) offering high throughput. Therefore, the main goal of this study was to demonstrate size-based membrane separation and bio-detection using double layer channel developed in our lab and to show how the membrane integrated channel can selectively separate rod shape cell separation with various aspect ratio based on size and enhance bio detection rate with flow. Based on an existing double-channel and cross-flow microfluidics platform, we explored various polymeric materials for fabricating porous membranes to use in pore-size-dependent separation. We induced pores via stop-flow lithography, and investigated membrane properties and limitations for pore-size-dependent separation. We investigated potential applications of poly(ethylene glycol) diacrylate (PEGDA)-based membrane integrated platforms in biological molecule detection based on streptavidin and biotin interaction. We demonstrated that flow and concentrations can enhance target detection in this platform.

scholarly journals Development of membrane in microfluidic device for sorting and detection for potential heterogeneity investigation

2021 ◽  
Author(s):  
Dehi Joung
Keyword(s):  
Pore Size ◽  
Mechanical Stability ◽  
Membrane Separation ◽  
Cross Flow ◽  
Polymeric Materials ◽  
Membrane Properties ◽  
Glycol Diacrylate ◽  
Recognition Element ◽  
Size Dependent ◽  
Biological Recognition

Membrane fabrication and integration with microfluidic devices has received increased attention for applications including bio-detection (a device providing analytical information in a selective and quantitative manner using a biological recognition element), membrane-based separation, and biological sample purification. The main challenges associated with these applications have been: 1) meeting sensitivity/selectivity requirements, 2) decreasing costs, 3) maintaining the mechanical stability of the membrane, 4) offering high throughput. Therefore, the main goal of this study was to demonstrate size-based membrane separation and bio-detection using double layer channel developed in our lab and to show how the membrane integrated channel can selectively separate rod shape cell separation with various aspect ratio based on size and enhance bio detection rate with flow. Based on an existing double-channel and cross-flow microfluidics platform, we explored various polymeric materials for fabricating porous membranes to use in pore-size-dependent separation. We induced pores via stop-flow lithography, and investigated membrane properties and limitations for pore-size-dependent separation. We investigated potential applications of poly(ethylene glycol) diacrylate (PEGDA)-based membrane integrated platforms in biological molecule detection based on streptavidin and biotin interaction. We demonstrated that flow and concentrations can enhance target detection in this platform.

scholarly journals Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Kostyantyn V Dmytruk ◽  
Oleh V Smutok ◽  
Olena V Dmytruk ◽  
Wolfgang Schuhmann ◽  
Andriy A Sibirny
Keyword(s):  
Hansenula Polymorpha ◽  
Recognition Element ◽  
Biological Recognition

scholarly journals Surface plasmon resonance and its application to biomedical research

Medicina ◽  
2007 ◽  
Vol 43 (5) ◽  
pp. 355 ◽  
Author(s):  
Asta Kaušaitė ◽  
Almira Ramanavičienė ◽  
Viktoras Mostovojus ◽  
Arūnas Ramanavičius
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Biomedical Research ◽  
Plasmon Resonance ◽  
Active Substance ◽  
Biologically Active ◽  
Biologically Active Substance ◽  
Recognition Element ◽  
Similar Chemical ◽  
Biological Recognition

In the recent years, surface plasmon resonance (SPR) has become one of the major methods for studying and determination of biologically active materials exhibiting affinity interactions. SRP biosensors are increasingly used in biochemistry and bioanalytical chemistry to determine antibody-antigen interactions, to investigate DNA hybridization, to diagnose bacteria- and virus-induced diseases, to identify hormones, steroids, and immunoglobulins, to investigate blood plasma coagulation. Using SPR biosensors, it is possible to analyze the mixtures of substances with a very similar chemical structure because SPR allows identifying only those analytes that specifically interact with biologically active substance immobilized on the surface of SPR biosensor. SPR biosensors are applied to monitor interactions between immobilized biologically active substance and analyte in real-time without labeling. On the other hand, it is possible to investigate not only association of analyte with immobilized material, but also the dissociation of a newly formed complex. SPR biosensors in many cases may be used to perform up to 50 measurements with the same SPR chip with an immobilized biological recognition element. Therefore, at present SPR is one of the most promising methods for determining the interactions between ligand and receptor, antigen and antibody, thus being increasingly used in diagnostics and biomedical research.

scholarly journals Nanopore sensor for copper ion detection using a polyamine decorated β-cyclodextrin as the recognition element

RSC Advances ◽  
2017 ◽  
Vol 7 (25) ◽  
pp. 15315-15320 ◽  
Author(s):  
Yanli Guo ◽  
Feifei Jian ◽  
Xiaofeng Kang
Keyword(s):  
Copper Ion ◽  
Ion Detection ◽  
Recognition Element

A novel and simple nanopore sensing method has been developed for the detection of CuII ions using polyamine decorated cyclodextrin as the recognition element.

An Electrokinetically Controlled DNA Hybridization Chip

2004 ◽  
Author(s):  
David Erickson ◽  
Xuezhu Liu ◽  
Ulrich Krull ◽  
Dongqing Li
Keyword(s):  
Dna Hybridization ◽  
Mass Transfer Rate ◽  
Channel Structure ◽  
Nucleic Acid Hybridization ◽  
Recognition Element ◽  
Delivery Technique ◽  
Biological Recognition ◽  
Long Time ◽  
On Line ◽  
Time Required

Biosensors and more specifically biochips exploit the interactions between a target analyte and an immobilized biological recognition element to produce a measurable signal. Systems based on surface phase nucleic acid hybridization, such as modern microarrays, are particularly attractive due to the high degree of selectivity in the binding interactions. One drawback of this reaction is the relatively long time required for complete hybridization to occur, as a result of the diffusion limited reaction kinetics. In this work an electrokinetically controlled DNA hybridization microfluidic chip will be introduced. The electrokinetic delivery technique provides the ability to dispense controlled sample sizes to the hybridization array while serving to increase the mass transfer rate and therefore the reaction speed. The focus of this paper will be on the design and microfabrication of the chip, the unique H-type channel structure and electrokinetic sample delivery and washing technique, and development of the on-line hybridization scanning. Initial hybridization results presented here demonstrate that less than 5 minutes and 4.9nL of 0.5μM ssDNA sample was required (35s dispensing period followed by a 4 minute wash) for complete hybridization.

Biological Recognition Element

2019 ◽  
Author(s):  
Ján Labuda ◽  
Richard P. Bowater ◽  
Miroslav Fojta ◽  
Günter Gauglitz ◽  
Zdeněk Glatz ◽  
...  
Keyword(s):  
Recognition Element ◽  
Biological Recognition
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