Electrochemical detection of Mycobacterium tuberculosis IS6110 gene fragments based on the gold nanocrystals with uniform morphology and highly exposed high-index facets and target DNA-induced recycling amplification

2020 ◽  
Vol 314 ◽  
pp. 128061
Author(s):  
Peng Yuanfeng ◽  
Li Ruiyi ◽  
Xie Qingqing ◽  
Chen Xiaofen ◽  
Yang Yongqiang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Electrochemical Detection ◽  
High Index ◽  
Gold Nanocrystals ◽  
Target Dna ◽  
Recycling Amplification

Growth of Tetrahexahedral Gold Nanocrystals with High-Index Facets

2009 ◽  
Vol 131 (45) ◽  
pp. 16350-16351 ◽  
Author(s):  
Tian Ming ◽  
Wei Feng ◽  
Qin Tang ◽  
Feng Wang ◽  
Lingdong Sun ◽  
...  
Keyword(s):  
High Index ◽  
Gold Nanocrystals

Ultrasensitive electrochemical detection of Hg2+based on an Hg2+-triggered exonuclease III-assisted target recycling strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5771-5778 ◽  
Author(s):  
Xiaolei Song ◽  
Yu Wang ◽  
Su Liu ◽  
Xue Zhang ◽  
Haiwang Wang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Electrochemical Method ◽  
Sensitive Detection ◽  
Mercury Ions ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Recycling Amplification ◽  
Highly Sensitive Detection

An isothermal electrochemical method for the highly sensitive detection of mercury ions (Hg2+) was established based on Hg2+-triggered exonuclease III-aided target recycling amplification.

Highly effective molecule converting strategy based on enzyme-free dual recycling amplification for ultrasensitive electrochemical detection of ATP

2017 ◽  
Vol 53 (59) ◽  
pp. 8368-8371 ◽  
Author(s):  
Hua Xie ◽  
Yaqin Chai ◽  
Yali Yuan ◽  
Ruo Yuan
Keyword(s):  
Electrochemical Detection ◽  
Highly Effective ◽  
Catalytic Hairpin Assembly ◽  
Recycling Amplification

An enzyme-free and highly effective molecule converting strategy was described for the sensitive electrochemical detection of ATP based on target-driven catalytic hairpin assembly and Mg2+-dependent DNAzymes.

scholarly journals Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn552′phoA In Vitro Transposition System

2000 ◽  
Vol 182 (10) ◽  
pp. 2732-2740 ◽  
Author(s):  
Miriam Braunstein ◽  
Thomas J. Griffin ◽  
Jordan I. Kriakov ◽  
Sarah T. Friedman ◽  
Nigel D. F. Grindley ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Protective Immunity ◽  
Cell Envelope ◽  
Open Reading Frames ◽  
Signal Peptides ◽  
Genomic Libraries ◽  
Target Dna ◽  
Genes Encoding ◽  
Associated Proteins

ABSTRACT Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552′phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the transposon inserts randomly into target DNA. M. tuberculosis genomic libraries were targeted with Tn552′phoA transposons, and these libraries were screened in M. smegmatis for active PhoA translational fusions. Thirty-two different M. tuberculosis open reading frames were identified; eight contain standard signal peptides, six contain lipoprotein signal peptides, and seventeen contain one or more transmembrane domains. Four of these proteins had not yet been assigned as exported proteins in the M. tuberculosisdatabases. This collection of exported proteins includes factors that are known to participate in the immune response of M. tuberculosis and proteins with homologies, suggesting a role in pathogenesis. Nine of the proteins appear to be unique to mycobacteria and represent promising candidates for factors that participate in protective immunity and virulence. This technology of creating comprehensive fusion libraries should be applicable to other organisms.

scholarly journals A PCR-Colorimetric Microwell Plate Hybridization Assay for Detection of Mycobacterium tuberculosis and M. avium from Culture Samples and Ziehl-Neelsen-Positive Smears

2000 ◽  
Vol 38 (5) ◽  
pp. 1772-1776 ◽  
Author(s):  
Maria Cristina Rossi ◽  
Andrea Gori ◽  
Gianguglielmo Zehender ◽  
Giulia Marchetti ◽  
Giulio Ferrario ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Lymph Node Biopsy ◽  
Pericardial Fluid ◽  
Hybridization Assay ◽  
Rrna Gene ◽  
Mycobacterial Infections ◽  
Detection Assay ◽  
Alternative Approach ◽  
Target Dna ◽  
Microwell Plate

Differentiation between Mycobacterium tuberculosis andM. avium is essential for the treatment of mycobacterial infections. We have developed an easy and rapid detection assay for the diagnosis of mycobacterial diseases. This is a PCR-hybridization assay based on selective amplification of a 16S rRNA gene sequence using pan-Mycobacteriumprimers followed by hybridization of the amplification products to biotinylated M. tuberculosis and M. avium-specific probes. A total of 55 mycobacterial isolates were tested. For all isolates, results concordant with those of conventional identification methods were obtained. Moreover, we developed a method for extraction of DNA from Ziehl-Neelsen-positive smears which allows the recovery of intact target DNA in our PCR-hybridization assay. Our method was able to confirm all culture results for 59 Ziehl-Neelsen-positive smears from clinical specimens (35 sputum, 11 lymph node biopsy, 6 stool, 4 pus, 2 urine, and 1 pericardial fluid specimens). These data suggest that our PCR-hybridization assay, which is simple to perform and less expensive than commercial probe methods, may be suitable for the identification of M. tuberculosis and M. avium. It could become a valuable alternative approach for the diagnosis of mycobacterial infections when applied directly to DNA extracted from Ziehl-Neelsen-positive smears as well.

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