Absolute quantification of cholesteryl esters using liquid chromatography-tandem mass spectrometry uncovers novel diagnostic potential of urinary sediment

Steroids ◽  
2017 ◽  
Vol 123 ◽  
pp. 43-49 ◽  
Author(s):  
Yusuke Miura ◽  
Takayuki Furukawa ◽  
Miho Kobayashi ◽  
Rojeet Shrestha ◽  
Ryoji Takahashi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Absolute Quantification ◽  
Tandem Mass ◽  
Cholesteryl Esters ◽  
Urinary Sediment ◽  
Chromatography Tandem Mass Spectrometry ◽  
Diagnostic Potential ◽  
Liquid Chromatography Tandem Mass

scholarly journals Comparison of Electrospray Ionization and Atmospheric Chemical Ionization Coupled with the Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Cholesteryl Esters

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Hae-Rim Lee ◽  
Sunil Kochhar ◽  
Soon-Mi Shim
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Signal Intensity ◽  
Chemical Ionization ◽  
Tandem Mass ◽  
Cholesteryl Esters ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Precursor Ions

The approach of two different ionization techniques including electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was tested for the analysis of cholesteryl esters (CEs). The retention time (RT), signal intensity, protonated ion, and product ion of CEs were compared between ESI and APCI. RT of CEs from both ionizations decreased with increasing double bonds, while it increased with longer carbon chain length. The ESI process generated strong signal intensity of precursor ions corresponding to [M+Na]+and [M+NH4]+regardless of the number of carbon chains and double bonds in CEs. On the other hand, the APCI process produced a protonated ion of CEs [M+H]+with a weak signal intensity, and it is selectively sensitive to detect precursor ions of CEs with unsaturated fatty acids. The ESI technique proved to be effective in ionizing more kinds of CEs than the APCI technique.

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