A handheld laser-induced fluorescence detector for multiple applications

Talanta ◽  
2016 ◽  
Vol 150 ◽  
pp. 135-141 ◽  
Author(s):  
Xiao-Xia Fang ◽  
Han-Yang Li ◽  
Pan Fang ◽  
Jian-Zhang Pan ◽  
Qun Fang
Keyword(s):  
Laser Induced Fluorescence ◽  
Fluorescence Detector

Determination of metolcarb in food by capillary electrophoresis immunoassay with a laser-induced fluorescence detector

2012 ◽  
Vol 33 (9-10) ◽  
pp. 1471-1476 ◽  
Author(s):  
Cuicui Liu ◽  
Guozhen Fang ◽  
Qiliang Deng ◽  
Yan Zhang ◽  
Jingjing Feng ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Laser Induced Fluorescence ◽  
Fluorescence Detector

Determination of NADH in Frozen Rat Brain Sections by Laser-Induced Fluorescence

2001 ◽  
Vol 382 (12) ◽  
pp. 1727-1732 ◽  
Author(s):  
André Rex ◽  
Lutz Pfeifer ◽  
Heidrun Fink
Keyword(s):  
Rat Brain ◽  
Metabolic Activity ◽  
Nicotinamide Adenine Dinucleotide ◽  
Brain Structures ◽  
Laser Induced Fluorescence ◽  
Fluorescence Detector ◽  
Nadh Fluorescence ◽  
Neurotoxic Lesions ◽  
Analytical Tools

Abstract Methods to assess metabolism are important analytical tools in neuroscience. The fluorophore nicotinamide adenine dinucleotide (NADH) is a parameter of cellular metabolism. NADH fluorescence was measured using a laserbased fluorescence detector with spectral and temporal filters. Distribution and intensity of NADH fluorescence were investigated in frozen brain sections. In sections containing hippocampus the intensity of NADH fluorescence was correlated to brain structures. In order to investigate the consequences of neurotoxic lesions, 5,7-dihydroxytryptamine was injected into the dorsal raphe nucleus 4 to 240 days prior to the measurement. NADH fluorescence decreased in the affected region by 50%, indicating that no recovery in metabolic activity had occurred.

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