Development and validation of a micro-QuEChERS method with high-throughput enhanced matrix removal followed with UHPLC-QqQ-MS/MS for analysis of raspberry ketone-related phenolic compounds in adipose tissues

Talanta ◽  
2021 ◽  
pp. 122716
Author(s):  
Bo Yuan ◽  
Danyue Zhao ◽  
Weiting Lyu ◽  
Zhiya Yin ◽  
Dushyant Kshatriya ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Throughput ◽  
Quechers Method ◽  
Adipose Tissues ◽  
Raspberry Ketone ◽  
Matrix Removal ◽  
Development And Validation

High-throughput modified QuEChERS method for the determination of the mycotoxin tenuazonic acid in wine grapes

RSC Advances ◽  
2016 ◽  
Vol 6 (98) ◽  
pp. 95670-95679 ◽  
Author(s):  
Ariel R. Fontana ◽  
Luciana P. Prendes ◽  
Vilma I. Morata ◽  
Rubén Bottini
Keyword(s):  
High Throughput ◽  
Tenuazonic Acid ◽  
Quechers Method ◽  
Wine Grapes ◽  
Modified Quechers ◽  
Development And Validation

Development and validation of a robust QuEChERS method for the quantification of tenuazonic acid in grapes.

scholarly journals Microwave-Assisted Extraction of Phenolics from Hibiscus sabdariffa Flowers: Method Development and Validation

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 51
Author(s):  
Rohmah Nur Fathimah ◽  
Widiastuti Setyaningsih ◽  
Ceferino Carrera ◽  
Miguel Palma
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Solvent Composition ◽  
Positive Contribution ◽  
Hibiscus Sabdariffa ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Development And Validation

Phenolics are bioactive compounds already reported in Hibiscus sabdariffa (roselle). The present study reports the development and validation of the analytical microwave-assisted extraction (MAE) method for the determination of phenolic compounds in roselle flowers to establish their positive contribution to human health. Prior to the optimization, a study for identifying phenolic compounds revealed that chlorogenic acid, protocatechuic acid, caffeic acid, and rutin were presented in Roselle. Three factors affecting MAE, viz. temperature, solvent composition, and sample to solvent ratio, were optimized employing a Box-Behnken Design (BBD) in conjunction with response surface methodology (RSM). The maximum extraction recovery was achieved using the extraction temperature of 68 °C, solvent composition of 59% MeOH in water, and 20:1 solvent to sample ratio. The kinetics experiment confirmed full recoveries (92–119%) at 15 min. Subsequently, method validation showed a satisfactory result, including low detection limits from 0.219 mg L−1 (caffeic acid) to 0.374 mg L−1 (chlorogenic acid). Both precisions and accuracy met the acceptances by AOAC. Finally, the method was successfully applied to quantify phenolics in the two most common varieties of Roselle. Chlorogenic acid was found as the main phenolic compound in both varieties

scholarly journals The development and validation of EpiComet-Chip, a modified high-throughput comet assay for the assessment of DNA methylation status

2017 ◽  
Vol 58 (7) ◽  
pp. 508-521 ◽  
Author(s):  
Todd A. Townsend ◽  
Marcus C. Parrish ◽  
Bevin P. Engelward ◽  
Mugimane G. Manjanatha
Keyword(s):  
Dna Methylation ◽  
Comet Assay ◽  
High Throughput ◽  
Methylation Status ◽  
Development And Validation

scholarly journals Development and Validation of an Automated High-Throughput System for Zebrafish In Vivo Screenings

PLoS ONE ◽  
2012 ◽  
Vol 7 (5) ◽  
pp. e36690 ◽  
Author(s):  
Ainhoa Letamendia ◽  
Celia Quevedo ◽  
Izaskun Ibarbia ◽  
Juan M. Virto ◽  
Olaia Holgado ◽  
...  
Keyword(s):  
High Throughput ◽  
Development And Validation
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