Diagnostic Performance of a Rapid Immunochromatographic Test Kit for Detecting Canine Parvovirus Infection

2021 ◽  
pp. 100551
Author(s):  
Felix K. Shima ◽  
Fidelis A. Gberindyer ◽  
Matthew T. Tion ◽  
Olusegun A. Fagbohun ◽  
Temidayo O. Omobowale ◽  
...  
Keyword(s):  
Diagnostic Performance ◽  
Canine Parvovirus ◽  
Immunochromatographic Test ◽  
Test Kit

scholarly journals Evaluation of a rapid immunochromatographic test kit to the gold standard fluorescent antibody test for diagnosis of rabies in animals in Bhutan

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Tenzin Tenzin ◽  
Kelzang Lhamo ◽  
Purna B. Rai ◽  
Dawa Tshering ◽  
Pema Jamtsho ◽  
...  
Keyword(s):  
Gold Standard ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Immunochromatographic Test ◽  
Test Kit

scholarly journals Prevalence of canine Parvo virus infection in street dogs using rapid antigen detection Kit

2015 ◽  
Vol 2 (3) ◽  
pp. 459-464
Author(s):  
Faizul Wasima Nahat ◽  
Md Siddiqur Rahman ◽  
Roma Rani Sarker ◽  
Md Kumrul Hassan ◽  
AKM Zeaul Hasan ◽  
...  
Keyword(s):  
Virus Infection ◽  
Infectious Diseases ◽  
Demographic Variables ◽  
Antigen Detection ◽  
Canine Parvovirus ◽  
Test Kit ◽  
Rectal Swabs ◽  
Chisquare Test

Canine parvovirus is one of the most common infectious diseases of dogs. A study was carried out to diagnose the infection of canine parvovirus in street dogs from different places of Mymensingh Town. Rectal samples were collected from January to April, 2015. A total of 114 rectal swabs were collected conveniently from street dogs of Mymensingh. The samples were diagnosed using RapiGEN Canine Parvo Virus Ag Test Kit. The association of CPV infection with demographic variables was assessed by Chisquare test. The overall prevalence of CPV was 32.0% in dogs. The prevalence of parvovirus infection was found to be significantly higher in puppies and 6 months of age (58.3%) than those >24 months of age (p=0.005). The prevalence of canine parvovirus infection also varied significantly in different study area (p=0.003). The prevalence of canine parvovirus infection was higher in male (34.4%) than that in female (30.2%) but it was not statistically significant.Res. Agric., Livest. Fish.2(3): 459-464, December 2015

scholarly journals Applications of a Rapid and Sensitive Dengue DUO Rapid Immunochromatographic Test Kit as a Diagnostic Strategy during a Dengue Type 2 Epidemic in an Urban City

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0158437 ◽  
Author(s):  
Hsin-I Shih ◽  
Hsiang-Chin Hsu ◽  
Chi-Jung Wu ◽  
Chih-Hao Lin ◽  
Chia-Ming Chang ◽  
...  
Keyword(s):  
Diagnostic Strategy ◽  
Immunochromatographic Test ◽  
Urban City ◽  
Test Kit

scholarly journals Diagnostic performance of a commercial ELISA used as a complementary test for bovine tuberculosis in two bovine herds with different disease status

2020 ◽  
Vol 72 (1) ◽  
pp. 1-8
Author(s):  
P.M. Soares Filho ◽  
A.K. Ramalho ◽  
A.M. Silva ◽  
M.A. Issa ◽  
P.M.P.C. Mota ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Diagnostic Performance ◽  
Latent Class ◽  
Serological Test ◽  
Antibody Test ◽  
Latent Class Models ◽  
Lower Sensitivity ◽  
Kappa Index ◽  
Serological Tests ◽  
Test Kit

ABSTRACT Bovine tuberculosis is a worldwide spread zoonotic disease. Intradermal tuberculinizations are the most used diagnostic tests in the world. Serological tests can be an ancillary diagnosis for bovine tuberculosis. The objective of this study was to evaluate the diagnostic performance of the ELISA Mycobacterium Bovis Antibody Test Kit IDEXX ™ in infected herds, which were in different disease control stages. One hundred and twenty animals from two dairy herds of Minas Gerais state, Brazil, were subjected to the ELISA serological test and the comparative cervical tuberculin test (CCT). Diagnostic test parameters were estimated using Bayesian latent class models and concordance between tests estimated by the frequentist approach. The ELISA test presented lower sensitivity than CCT in both herds. Its sensitivity was higher in the herd in sanitation process. Specificity estimates were above 95% in both herds. Kappa index indicated low concordance or even disagreement between tests. According to the results, the ELISA IDEXX should not be used as substitution for CCT. The tests must not be associated in series. Parallel association increased diagnostic sensitivity in the herd which was in the process of sanitation.

scholarly journals Rapid Serological Tests for SARS-COV-2: Diagnostic Performance of Four Commercial Assays

2020 ◽  
Author(s):  
Sérgio Monteiro de Almeida ◽  
Regiane Nogueira Spalanzani ◽  
Meri Bordignon Nogueira ◽  
Beatriz Sanada Spiri ◽  
Barbara Maria Cavalli ◽  
...  
Keyword(s):  
Diagnostic Performance ◽  
Rapid Test ◽  
Nucleic Acid Amplification ◽  
Control Group ◽  
Serum Samples ◽  
Serological Tests ◽  
Test Kit ◽  
Healthy Control ◽  
One Step ◽  
Substantial Heterogeneity

Abstract Background. This study aimed to assess the diagnostic performance of lateral flow immunochromatographic assays (LFA) of four different manufacturers to identify SARS-CoV-2 antibodies (IgM, IgG or total), comparing them with the nucleic acid amplification test (NAAT) or clinical defined (definite or probable SARS-CoV-2 infection respectively). Methods. 119 serum samples were randomly selected by convenience and distributed in the groups: (1) Group with SARS-CoV-2 infection [n=82; RT-qPCR positive (definite, n=70), and probable (n=12)]; (2) other diseases [n= 27; other viruses identified (n=8), SARS of other etiologies (n=19)]; (3) healthy control group (n=10). LFA essays of four manufacturers were compared: MedTest Coronavírus (COVID-19) IgG/IgM (MedLevensohn, Brazil); COVID-19 IgG/IgM ECO Test (Ecodiagnóstica, Brazil); Camtech COVID-19 IgM/IgG Rapid Test Kit (Camtech Diagnostics Pte Ltd, Singapore); and one Step COVID-19 Test for total antibodies (Guangzhou Wondfo Biotech Co, China).Results. The four tests studied showed high diagnostic performance characteristics for the diagnoses of definite or probable SARS-CoV-2 infection. The best measures were for the Wondfo test: sensitivity (86.59%; 95%CI, 77.26-93.11%); specificity (100%; 90.51-100%); DOR (257; 60-1008); LR+ (33.43; 4.82-231.85); LR− (0.13; 0.08 - 0.23); accuracy (90.76%; 84.06- 95.29%); Matthews Correlation coefficient (MCC) 0.82. Although considering only the probable SARS-CoV-2 infection (PCR-) cases, all the kits studied showed limited values.Conclusion. Our data demonstrate the excellent performance of LFA for the diagnoses of definite or probable SARS-CoV-2 infection. There was substantial heterogeneity in sensitivities of IgM and IgG antibodies among the manufacturers. LFA tests cannot replace molecular diagnostics, but should be used as additional screening tool.

scholarly journals Rapid Serological Tests for SARS-COV-2: Diagnostic Performance of Four Commercial Assays

2020 ◽  
Author(s):  
Sérgio Monteiro de Almeida ◽  
Regiane Nogueira Spalanzani ◽  
Meri Bordignon Nogueira ◽  
Beatriz Sanada Spiri ◽  
Barbara Maria Cavalli ◽  
...  
Keyword(s):  
Diagnostic Performance ◽  
Rapid Test ◽  
Nucleic Acid Amplification ◽  
Control Group ◽  
Serum Samples ◽  
Serological Tests ◽  
Test Kit ◽  
Healthy Control ◽  
One Step ◽  
Substantial Heterogeneity

Abstract Background. This study aimed to assess the diagnostic performance of lateral flow immunochromatographic assays (LFA) of four different manufacturers to identify SARS-CoV-2 antibodies (IgM, IgG or total), comparing them with the nucleic acid amplification test (NAAT) or clinical defined (definite or probable SARS-CoV-2 infection respectively). Methods. 119 serum samples were randomly selected by convenience and distributed in the groups: (1) Group with SARS-CoV-2 infection [n=82; RT-qPCR positive (definite, n=70), and probable (n=12)]; (2) other diseases [n= 27; other viruses identified (n=8), SARS of other etiologies (n=19)]; (3) healthy control group (n=10). LFA essays of four manufacturers were compared: MedTest Coronavírus (COVID-19) IgG/IgM (MedLevensohn, Brazil); COVID-19 IgG/IgM ECO Test (Ecodiagnóstica, Brazil); Camtech COVID-19 IgM/IgG Rapid Test Kit (Camtech Diagnostics Pte Ltd, Singapore); and one Step COVID-19 Test for total antibodies (Guangzhou Wondfo Biotech Co, China).Results. The four tests studied showed high diagnostic performance characteristics for the diagnoses of definite or probable SARS-CoV-2 infection. The best measures were for the Wondfo test: sensitivity (86.59%; 95%CI, 77.26-93.11%); specificity (100%; 90.51-100%); DOR (257; 60-1008); LR+ (33.43; 4.82-231.85); LR− (0.13; 0.08 - 0.23); accuracy (90.76%; 84.06- 95.29%); Matthews Correlation coefficient (MCC) 0.82. Although considering only the probable SARS-CoV-2 infection (PCR-) cases, all the kits studied showed limited values.Conclusion. Our data demonstrate the excellent performance of LFA for the diagnoses of definite or probable SARS-CoV-2 infection. There was substantial heterogeneity in sensitivities of IgM and IgG antibodies among the manufacturers. LFA tests cannot replace molecular diagnostics, but should be used as additional screening tool.

scholarly journals Prevalence of canine parvo virus and canine influenza virus infection in dogs in Dhaka, Mymensingh, Feni and Chittagong districts of Bangladesh

2016 ◽  
Vol 2 (1) ◽  
pp. 138-142 ◽  
Author(s):  
Soumitra Sen ◽  
Md Siddiqur Rahman ◽  
Minakshi Nag ◽  
Mohummad Muklesur Rahman ◽  
Roma Rani Sarker ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Virus Infection ◽  
Influenza Virus Infection ◽  
Canine Parvovirus ◽  
Age Group ◽  
Pet Dogs ◽  
Canine Influenza Virus ◽  
Test Kit ◽  
Canine Influenza ◽  
Age And Sex

Canine parvovirus (CPV) and canine influenza virus (CIV) are highly contagious virus infecting members of the canine family including dogs, coyotes, foxes and wolves. The research work was carried out to determine the prevalence of CPV and CIV in dogs (stray and pet dogs) in Dhaka, Mymensingh, Feni and Chittagong Districts by using Rapigen CPV and CIV Ag test kit. Fecal samples were collected from 50 dogs of different age and sex from different spots of Dhaka and Chittagong District. Nasal fluid samples were collected from 50 dogs of different age and sex from different spots. The samples were tested with Rapigen canine parvovirus Ag test kit and Rapigen canine influenza Ag test kit. Overall prevalence of CPV in pet dogs was 22% and in stray dogs was 30%. The prevalence of canine parvovirus in relation to age was gradually decreasing with higher age group and the prevalence was 28% in 1-6 months age, 16.66% in 7-12 months age group 11.11%o ver 18 months age group. The prevalence of canine parvovirus was significantly higher in male (24.13%) than that in female (19.04%). All samples showed negative test result for canine influenza virus. Prevalence of canine influenza virus infection were 00.00% in all age groups and sex. Overall prevalence of CIV in all pet and stray dogs were 00.00%.Asian J. Med. Biol. Res. March 2016, 2(1): 138-142

Diagnostic performance of the noninvasive prenatal FetoGnost RhD assay for the prediction of the fetal RhD blood group status

2021 ◽  
Author(s):  
Tobias J. Legler ◽  
Sandra Lührig ◽  
Irina Korschineck ◽  
Dieter Schwartz
Keyword(s):  
Diagnostic Accuracy ◽  
Diagnostic Performance ◽  
Clinical Decision Making ◽  
False Negative ◽  
Clinical Decision ◽  
Multiple Pregnancies ◽  
Group Status ◽  
Test Kit ◽  
Existing Data

Abstract Purpose: To evaluate the diagnostic accuracy of a commercially available test kit for noninvasive prenatal determination of the fetal RhD status (NIPT-RhD) with a focus on early gestation and multiple pregnancies. Methods: The FetoGnost RhD assay (Ingenetix, Vienna, Austria) is routinely applied for clinical decision making either in woman with anti-D alloimmunization or in order to target the application of routine antenatal anti-D prophylaxis (RAADP) to women with a RhD positive fetus. Based on existing data in the laboratory information system the newborn’s serological RhD status was compared with NIPT RhD results. Results: Since 2009 NIPT RhD was performed in 2,968 pregnant women between week 5+6 and 40+0 of gestation (median 12+6) and conclusive results were obtained in 2,888 (97.30%) cases. Diagnostic accuracy was calculated from those 2244 (77.70%) cases with the newborn’s serological RhD status reported. The sensitivity of the FetoGnost RhD assay was 99.93% (95% CI 99.61% - 99.99%) and the specificity was 99.61% (95% CI 98.86% - 99.87%). No false positive or false negative NIPT RhD result was observed in 203 multiple pregnancies. Conclusion: NIPT RhD results are reliable when obtained with FetoGnost RhD assay. Targeted routine anti-D-prophylaxis can start as early as 11+0 weeks of gestation in singleton and multiple pregnancies.

scholarly journals Reveal Q+ MAX® for Detection of Total Af latoxin in Corn, Almonds, Pistachios, Walnuts, and Peanuts

2019 ◽  
Vol 102 (2) ◽  
pp. 525-531 ◽  
Author(s):  
Quynh-Nhi Le ◽  
Brooke Roman ◽  
Dana Driksna ◽  
Lindsey Gilbert ◽  
Kaylee Gonzales ◽  
...  
Keyword(s):  
Room Temperature ◽  
Lateral Flow ◽  
Immunochromatographic Test ◽  
Total Aflatoxin ◽  
Average Percentage ◽  
Assay Performance ◽  
Flow Test ◽  
Test Kit ◽  
Lateral Flow Test ◽  
Stability Results

Abstract Background: The Reveal Q+ MAX for Aflatoxin is a lateral flow immunochromatographic test intended for quantitative analysis within 6 min after aqueous extraction. Objective: Work was conducted to validate the performance of the Reveal Q+ MAX for Aflatoxin method in selected corn and nut matrixes. Methods: This method was validated under the requirements of the AOAC Research Institute Performance Tested MethodSM program. Fivematrixes, including corn naturally contaminated with aflatoxin at 0, 5.2, 21.0, 51.6, 103.6, and 282 ppb as well as peanuts, pistachios, walnuts, and almonds spiked at 0, 5, 20, 50, and 300 ppb were analyzed. Results: Average percentage recoveries of the added aflatoxin from the matrixes ranged from 80.8 to 116.9%. Average LOD for all matrixes is 2 ppb and LOQ is 7 ppb. With the exceptionof sample size for almonds, robustness trials demonstrated that deliberate changes to the assay parameters minimally affected the Reveal Q+ MAX assay performance. Finally, stability results from three independently manufactured lots support Reveal Q+ MAX for Aflatoxin performance consistency and shelf-life of 18 months when stored at room temperature. Conclusions: This study appropriatelyvalidates the Performance Tested MethodSM claim forcorn and selected nut matrixes on Reveal Q+ MAX forAflatoxin, an aqueous lateral flow test kit. Highlights: Aqueous lateral flow test kit detects total aflatoxin between 80 to 120% yieldwith an LOD of 2 ppb.

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