Matrix-free three-dimensional culture of bovine secondary follicles to antral stage: Impact of media formulation and epidermal growth factor (EGF)

2022 ◽  
Author(s):  
Shaina L. Jachter ◽  
Wilson P. Simmons ◽  
Charles Estill ◽  
Jing Xu ◽  
Cecily V. Bishop
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Three Dimensional ◽  
Media Formulation ◽  
Epidermal Growth ◽  
Three Dimensional Culture ◽  
Matrix Free

scholarly journals A positively charged cluster in the epidermal growth factor-like domain of Factor VII-activating protease (FSAP) is essential for polyanion binding

2006 ◽  
Vol 394 (3) ◽  
pp. 687-692 ◽  
Author(s):  
Boran Altincicek ◽  
Aya Shibamiya ◽  
Heidi Trusheim ◽  
Eleni Tzima ◽  
Michael Niepmann ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Three Dimensional ◽  
Factor Vii ◽  
Dimensional Structure ◽  
Heparin Binding ◽  
Interaction Sites ◽  
Interaction Domains ◽  
Epidermal Growth

FSAP (Factor VII-activating protease) is a novel plasma-derived serine protease that regulates haemostasis as well as vascular cell proliferation. FSAP undergoes autoactivation in the presence of polyanionic macromolecules such as heparin and RNA. Competition experiments suggest that RNA and heparin bind to the same or overlapping interaction sites. A proteolysis approach, where FSAP was hydrolysed into smaller fragments, was used to identify the polyanion-binding site. The EGF (epidermal growth factor)-like domains EGF2 and EGF3 of FSAP are the major interaction domains for RNA. The amino acids Arg170, Arg171, Ser172 and Lys173 within the EGF3 domain were essential for this binding. This is also the region with the highest positive net charge in the protein and is most probably located in an exposed loop. It is also highly conserved across five species. Disruption of disulphide bridges led to the loss of RNA and heparin binding, indicating that the three-dimensional structure of the EGF3 domain is essential for binding to negatively charged heparin or RNA. The identification of polyanion-binding sites will help to define the role of FSAP in the vasculature.

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