scholarly journals Impact of various reagents during blood collection from poultry to analyze morpho-biochemical indicators

2021 ◽  
Vol 262 ◽  
pp. 02009
Author(s):  
Vladimir Vertiprakhov ◽  
Alena Grozina ◽  
Vladimir Fisinin ◽  
Natalya Ovchinnikova ◽  
Anatoly Bittirov
Keyword(s):  
Broiler Chickens ◽  
Blood Count ◽  
Sodium Citrate ◽  
Medical Science ◽  
White Blood Count ◽  
Blood Collection ◽  
Blood Samples ◽  
Biochemical Indicators ◽  
The Impact

The work provides experimental data on the impact of anticoagulants (K3 - EDTA, 3.8% sodium citrate and lithium heparin) and coagulation activator on morpho-biochemical indicators in the blood samples of broiler chickens of cross-selection of Agricultural Center “Smena” at the age of 35 days. The studies were carried out with the use of semi-automatic flow analyzer Sinnowa BS-3000P (SINNOWA Medical Science & Technology Co., Ltd, China) and veterinary automatic blood analyzer DF-50 by Dymind Biotech (PRC) with the use of original reagents. The results showed that the most optimal reagent for determination of morphological indicators of the poultry blood was EDTA. The average white blood count (WBC) is lower in blood samples with citrate and heparin in contrast to EDTA by 30.5% and 24.1%, respectively. The average red blood count (RBC) is lower in tubes with sodium citrate (by 37.9 %) and lithium heparin (by 13.8 %) in contrast to K3-EDTA. When the sodium citrate anticoagulant is used, the blood biochemistry results for all positions of the experiment, excluding calcium, are lower than in heparin, and its closest values are lower than in coagulation activator, excluding trypsin activity. That’s why, the most optimal reagents for biochemistry, including for blood enzyme tests, should be considered the tubes with heparin or coagulation activator.

1-Desamino-8-D-Arginine Vasopressin (DDAVP) Infusion in Type IIB von Willebrand’s Disease: Shortening of Bleeding Time and Induction of a Variable Pseudothrombocytopenia

1990 ◽  
Vol 64 (01) ◽  
pp. 117-120 ◽  
Author(s):  
Alessandra Casonato ◽  
M Teresa Sartori ◽  
Luigi de Marco ◽  
Antonio Girolami
Keyword(s):  
Monoclonal Antibody ◽  
Platelet Count ◽  
Arginine Vasopressin ◽  
Calcium Concentration ◽  
Sodium Citrate ◽  
Bleeding Time ◽  
Blood Collection ◽  
Von Willebrand's Disease ◽  
Blood Samples ◽  
Von Willebrand’S Disease

SummaryWe have investigated the effects of 1-desamino-8-D-arginine vasopressin (DDAVP) infusion on platelet count and bleeding time in 4 patients with type IIB von Willebrand’s disease (vWd). Three of four patients showed a normalization of the bleeding time within 1 h after the infusion, while bleeding time was not modified in the fourth. In accordance with the literature, thrombocytopenia was observed after DDAVP infusion, but this thrombocytopenia was due to the anticoagulants used for blood collection. In two patients (F. I., G. F.) no thrombocytopenia was observed when platelets were counted by fingerstick method but there was a 20% platelet decrease in blood samples collected in sodium citrate and a 50% decrease in samples collected in EDTA. Dramatic falls in platelet counts (70–95%) were observed in the additional two patients (C. A., D.Z.) after DDAVP infusion, when both sodium citrate or EDTA were used as anticoagulants. In the latter two patients there was also a 50% decrease in platelet count when the fingerstick method was used. The decrease in the patient’s platelet count in EDTA samples after DDAVP infusion could be prevented, in part, by the previous additions of an anti GPIb monoclonal antibody and an anti GPIIb-IIIa monoclonal antibody.Thus, the thrombocytopenia observed in the four IIB vWd patients studied after DDAVP infusion seems to be, at least partially, a pseudothrombocytopenia depending on the calcium concentration in the blood samples and the availability of GPIb and GPIIb-IIIa receptors. These findings and the normalization of the bleeding time observed in three of the four patients has led us to reconsider the possible use of DDAVP in the treatment of our IIB vWd patients.

Activation and measurement of plasma prorenin in the rat

1991 ◽  
Vol 69 (9) ◽  
pp. 1331-1340 ◽  
Author(s):  
P. Ioannou ◽  
A. Y. Loh ◽  
D. H. Osmond
Keyword(s):  
Human Plasma ◽  
Rat Plasma ◽  
Blood Collection ◽  
Angiotensin I ◽  
Renin Substrate ◽  
Blood Samples ◽  
Functional Component ◽  
Fresh Frozen ◽  
Normal Rat

Prorenin determination in rat plasma has been problematic from the outset. Consequently, its existence is questioned by some and its quantity by others, making it difficult for knowledge to advance as to its function relative to the renin system. The present study examines major variables in the determination of rat plasma prorenin and renin, notably different prorenin activation protocols involving blood samples obtained under various conditions from animals under different anesthetics. We found that a trypsin activation step with 5 mg/mL plasma, 60 min at 23 °C, followed by a PRA step of 10 min at 37 °C, resulted in the highest prorenin estimates, up to approximately 400 ng∙mL−1∙h−1 in terms of angiotensin I, as compared with published values of 0–190, based on other protocols. These estimates were obtained despite considerable destruction of angiotensinogen (renin substrate) by trypsin. Cryoactivation of prorenin was much less effective than in human plasma but, when followed by trypsin, it facilitated greater activation than with trypsin alone. Comparable fresh and fresh-frozen plasmas had similar prorenin–renin values, but lower values were observed in plasmas that had been repeatedly frozen and thawed. Conscious rats and those anesthetized with Inactin or ether had higher renins and prorenins than those anesthetized with methoxyflurane or halothane. Rats with kidneys in place during blood collection had higher renins (but not prorenins) than those whose kidneys were clamped off, suggesting that last-minute renin release during blood collection had occurred. We conclude that (i) trypsin generates increased renin, or renin-like, activity in plasma, suggesting activation of a precursor; (ii) on this basis, high prorenin levels exist in normal rat plasma; (iii) renin and prorenin levels are variously influenced by different anesthetics and blood handling procedures; (iv) variation in prorenin levels suggests that it is a dynamic (functional?) component of the renin system; (v) prorenin measurements are heavily influenced by methodological variations during the trypsin step or the subsequent PRA step; (vi) using standardized methodology, the rat can serve as a model for investigating the function of prorenin in normotension and hypertension.Key words: tryptic activation, angiotensinogen, adrenalectomy, anesthesia.

scholarly journals Evaluation of haematology analyzer CELL-DYN 3700 SL

2003 ◽  
Vol 3 (2) ◽  
pp. 35-41 ◽  
Author(s):  
Enver Suljević ◽  
Muhamed Fazlić ◽  
Jozo Čorić ◽  
Emina Kiseljaković
Keyword(s):  
Correlation Coefficient ◽  
Sensitivity And Specificity ◽  
Blood Count ◽  
White Blood Count ◽  
Full Blood Count ◽  
Distribution Width ◽  
Medical Laboratories ◽  
Analyzer Cell ◽  
Mean Corpuscular Haemoglobin

Research on the parameters of full blood count and differential white blood count is included in the program of all medical laboratories of primary, secondary and tertiary health care levels. Today, all haematological tests are exclusively performed on the haematology analyzers. Automation of haematology laboratories is a result of the huge requires for haematological test performing, timely issuing of the haematological findings, and possibility of the usage of modern techniques.This work is an evaluation of laser haematology analyzer Cell-Dyn 3700 SL. It investigates the reliability of test results throughout the following parameters: precision, accuracy, sensitivity and specificity of determination methods. It also explores the influence of sample transferring and correlation with haematology analyzer MAXM Retti. Haematology parameters that have been investigated are: white blood cell (WBC), neutrophils (NEU), lymphocytes (LXM), monocytes (MONO), eosinophils (EOS), basophils (BASO), red blood cells (RBC), haemoglobin (HGB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCHC) red cell distribution width (RDW), platelet (PLT), mean platelet volume (MPV), plateletocrit (PCT), and platelet distribution width (PDW).The results confirms that precision of analyzer fulfils the reproducibility of testing parameters: WBC, RBC, HGB, MCV, MCH, MCHC, and PLT. Correlation coefficient values (r) gained throughout the statistical analysis, that is linear regression results obtained throughout the comparison of two analyzers are adequate except for MCHC (r = 0.64), what is in accordance with literature data.Accuracy is tested by haematology analyzer method and microscopic differentiating method. Correlation coefficient results for granulocytes, lymphocytes and monocytes point the accuracy of methods. Sensitivity and specificity parameters fulfil the analytical criteria.It is confirmed that haematology analyzer Cell-Dyn 3700 SL is reliable for the determination of full blood count in everyday work. Analyzer and its program for differential white blood count can be used for the research and separation of normal and pathological blood counts with addition of microscopic methods confirming distribution or morphologic changes of leukocytes.

scholarly journals Assessing of mouflon biochemical parameters depending on genders

2018 ◽  
Author(s):  
Terézia Pošiváková ◽  
Rudolf Hromada ◽  
Jozef Švajlenka ◽  
Ján Pošivák
Keyword(s):  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Winter Season ◽  
Biochemical Parameter ◽  
Statistical Testing ◽  
Blood Samples ◽  
Biochemical Indicators ◽  
Experimental Group

The aim of our study was to evaluate the selected biochemical parameters of mouflon depending on gender. For research were used thirty mouflons of both sexes with approximate age in winter season. Blood samples for biochemical analysis were taken from vena jugularis for determination of selected biochemical parameter. Biochemical indicators were measured using the standard automatic analyser. The results of statistical testing of selected biochemical parameters in the experimental group of animals confirmed differences between the genders and at the selected biochemical parameters.

THE EFFECT OF ETHER ANESTHESIA ON PLASMA CORTICOSTEROIDS AND HEMATOLOGIC RESPONSES

1965 ◽  
Vol 43 (3) ◽  
pp. 463-468 ◽  
Author(s):  
M. C. Cann ◽  
Z. Z. Zawidzka ◽  
J. M. Airth ◽  
H. C. Grice
Keyword(s):  
Blood Count ◽  
White Blood Count ◽  
Differential Count ◽  
Ether Anesthesia ◽  
Blood Samples ◽  
Anesthetized Rats ◽  
Iliac Bifurcation

Blood samples taken from the aorta at the iliac bifurcation of ether-anesthetized rats were similar in plasma corticosteroids, white blood count, and differential count to those taken from rats by decapitation or by ether anesthesia and decapitation. These values were not affected by ether anesthesia of less than 2 minutes duration. The values were affected by continuous ether exposure of more than [Formula: see text] minutes duration up to 96 minutes.

scholarly journals Reference Range of Complete Blood Count in Healthy Term Newborns 1 Week after Birth

2021 ◽  
Vol 9 (A) ◽  
pp. 1565-1569
Author(s):  
Harapan Parlindungan Ringoringo
Keyword(s):  
Birth Weight ◽  
Standard Deviation ◽  
Blood Count ◽  
Reference Range ◽  
Complete Blood Count ◽  
White Blood Count ◽  
Inclusion Criteria ◽  
Term Newborns ◽  
Prospective Analyses

BACKGROUND: Determination of the complete blood count (CBC) and reticulocyte reference range helps diagnose a disease related to changes in erythrocyte indices, white blood count, platelets, and reticulocytes, especially in newborns. AIM: The aim study is to establish a reference range for CBC and reticulocyte in healthy term newborns 1 week after birth. MATERIAL AND METHODS: The study method is prospective analyses of CBC and reticulocyte in term newborns within 1 week after birth. Inclusion criteria are term newborn (gestational age 37–42 weeks) with good tone, breathing, or crying immediately, do not have resuscitation, and the birth weight is ≥2500 g. RESULTS: Two hundred and seventy-seven term newborns met the inclusion criteria. There were 145 (52.35%) male infants and 132 (47.65%) female infants. The reference range of newborns for hemoglobin (Hb) at P2.5–P97.5, P3–P97, P5–P95, and mean ± 2 standard deviation (SD) was 11.70–19.60 g/dL, 11.90–19.57 g/dL, 12.20–19.20 g/dL, and 11.35–19.63 g/dL, respectively. In addition, reference range of male newborns for Hb at P2.5–P97.5, P3–P97, P5–P95, and mean ± 2SD was 12.20–20.14 g/dL, 12.28–19.91 g/dL, 12.63–19.38 g/dL, and 11.47–20.15 g/dL, respectively. The reference range of female newborns for Hb at P2.5–P97.5, P3–P97, P5–P95, and mean ± 2 SD was 11.37–19.20 g/dL, 11.50–19.20 g/dL, 11.80–19.20 g/dL, and 11.37–18.93 g/dL, respectively. CONCLUSION: The reference range of CBC and reticulocyte for healthy term newborns, male newborns, and female newborns in this study can be used as a benchmark.

scholarly journals Early Effects of a Hypocaloric, Mediterranean Diet on Laboratory Parameters in Obese Individuals

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Marta Greco ◽  
Eusebio Chiefari ◽  
Tiziana Montalcini ◽  
Francesca Accattato ◽  
Francesco S. Costanzo ◽  
...  
Keyword(s):  
Weight Loss ◽  
Calorie Restriction ◽  
Blood Collection ◽  
Laboratory Parameters ◽  
Leptin Sensitivity ◽  
Common Strategy ◽  
Early Effects ◽  
Laboratory Biomarkers ◽  
The Impact

Calorie restriction is a common strategy for weight loss in obese individuals. However, little is known about the impact of moderate hypocaloric diets on obesity-related laboratory parameters in a short-term period. Aim of this study was to evaluate the variation of laboratory biomarkers in obese individuals following a Mediterranean, hypocaloric (1400–1600 Kcal/die) diet. 23 obese, pharmacologically untreated patients were enrolled and subjected to the determination of anthropometric variables and blood collection at baseline, 1 and 4 months after diet initiation. After 4 months of calorie restriction, we observed a significant decrease in body weight and BMI (bothP<0.0001), insulin (P=0.037), HOMA-IR (P=0.026), leptin (P=0.008), and LDH (P=0.023) and an increase in EGF (P=0.013). All these parameters, except LDH, varied significantly already at 1 month after diet initiation. Also, lower levels of insulin (P=0.025), leptin (P=0.023), and EGF (P=0.035) were associated with a greater (>5%) weight loss. Collectively, our data support a precocious improvement of insulin and leptin sensitivity after a modest calorie restriction and weight reduction. Moreover, EGF and LDH may represent novel markers of obesity, which deserve further investigations.

scholarly journals Valuation of three different anaesthetic protocols on complete blood count and biochemical parameters on Wistar rats

2018 ◽  
Vol 68 (4) ◽  
pp. 587 ◽  
Author(s):  
P. P. LELOVAS ◽  
M. S. STASINOPOULOU ◽  
E. G. BALAFAS ◽  
M. A. NIKITA ◽  
N. T. SISKOS ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Wistar Rats ◽  
Blood Count ◽  
Complete Blood Count ◽  
Vena Cava ◽  
Histological Evaluation ◽  
Blood Collection ◽  
Female Wistar Rats ◽  
The Impact ◽  
To Receive

The objective of the current study was to determine the impact of three different anaesthetic protocols on blood and biochemical parameters. Eighteen female Wistar rats (HsdOla:WI) 3 months old, weighting 197.09 ± 7.39 g were used. Baseline blood collection was performed in all animals from the lateral coccygeal vein for evaluation of glucose. The animals were then randomly allocated to receive one of three different anaesthetic protocols: dexmedetomidine/ketamine intramuscularly (0.25 mg/kg and 50 mg/kg respectively), or isoflurane 0.2 ml on cotton inside a syringe case, or isoflurane administered by vaporiser (5% induction and 2-3% maintenance of anaesthesia, delivered in oxygen flow 1 L/min). Blood samples were collected from caudal vena cava for complete blood count and biochemical analysis, while the lung and the liver were harvested for histological evaluation. Comparison between groups, as far as complete blood count parameters is concerned, revealed statistical significant differences in red blood cells, haematocrit and haemoglobin values with lower values being observed in vaporiser anaesthesia group. Furthermore, within group comparison revealed statistical significant differences for glucose in all three groups. No evidence of inflammatory, degenerative neoplastic or toxicity related lesions were observed during histological evaluation of the lung and liver.

scholarly journals Optimization of Preanalytical Variables for cfDNA Processing and Detection of ctDNA in Archival Plasma Samples

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Marijana Nesic ◽  
Julie S. Bødker ◽  
Simone K. Terp ◽  
Karen Dybkær
Keyword(s):  
Nucleic Acid ◽  
B Cell Lymphoma ◽  
Circulating Tumor Dna ◽  
Blood Collection ◽  
Plasma Samples ◽  
Cell Free Dna ◽  
Blood Samples ◽  
Free Dna ◽  
The Impact ◽  
Blood Collection Tubes

DNA released from cells into the peripheral blood is known as cell-free DNA (cfDNA), representing a promising noninvasive source of biomarkers that could be utilized to manage Diffuse Large B-Cell Lymphoma (DLBCL), among other diseases. The procedure for purification and handling of cfDNA is not yet standardized, and various preanalytical variables may affect the yield and analysis of cfDNA, including the purification kits, blood collection tubes, and centrifugation regime. Therefore, we aimed to investigate the impact of these preanalytical variables on the yield of cfDNA by comparing three different purification kits DNeasy Blood & Tissue Kit (Qiagen), QIAamp Circulating Nucleic Acid Kit (Qiagen), and Quick-cfDNA Serum & Plasma Kit (Zymo Research). Two blood collection tubes (BCTs), EDTA-K2 and Cell-Free DNA (Streck), stored at four different time points before plasma was separated and cfDNA purified, were compared, and for EDTA tubes, two centrifugation regimes at 2000 × g and 3000 × g were tested. Additionally, we have tested the utility of long-term archival blood samples from DLBCL patients to detect circulating tumor DNA (ctDNA). We observed a higher cfDNA yield using the QIAamp Circulating Nucleic Acid Kit (Qiagen) purification kit, as well as a higher cfDNA yield when blood samples were collected in EDTA BCTs, with a centrifuge regime at 2000 × g . Moreover, ctDNA detection was feasible from archival plasma samples with a median storage time of nine years.

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