Transforming eukaryotic cell culture with macromolecular crowding

2021 ◽  
Author(s):  
Michael Raghunath ◽  
Dimitrios I. Zeugolis
Keyword(s):  
Cell Culture ◽  
Macromolecular Crowding ◽  
Eukaryotic Cell

Use of a Quantitative TaqMan-PCR for the Fast Quantification of Mycobacteria in Broth Culture, Eukaryotic Cell Culture and Tissue

2003 ◽  
Vol 50 (10) ◽  
pp. 505-509 ◽  
Author(s):  
A. Lewin ◽  
B. Freytag ◽  
B. Meister ◽  
S. Sharbati-Tehrani ◽  
H. Schafer ◽  
...  
Keyword(s):  
Cell Culture ◽  
Eukaryotic Cell ◽  
Broth Culture ◽  
Taqman Pcr

Production of yeastolates for uniform stable isotope labelling in eukaryotic cell culture

2009 ◽  
Vol 84 (3) ◽  
pp. 575-581 ◽  
Author(s):  
T. A. Egorova-Zachernyuk ◽  
G. J. C. G. M. Bosman ◽  
A. M. A. Pistorius ◽  
W. J. DeGrip
Keyword(s):  
Cell Culture ◽  
Stable Isotope ◽  
Eukaryotic Cell ◽  
Stable Isotope Labelling ◽  
Isotope Labelling

scholarly journals Experimental and Theoretical Analysis of Metal Complex Diffusion through Cell Monolayer

Entropy ◽  
2021 ◽  
Vol 23 (3) ◽  
pp. 360
Author(s):  
Katarzyna Gałczyńska ◽  
Jarosław Rachuna ◽  
Karol Ciepluch ◽  
Magdalena Kowalska ◽  
Sławomir Wąsik ◽  
...  
Keyword(s):  
Cell Culture ◽  
Quantitative Analysis ◽  
Cell Monolayer ◽  
Laser Interferometry ◽  
Eukaryotic Cell ◽  
Monolayer Cell Culture ◽  
Cell Monolayers ◽  
Essential Step ◽  
Complex Diffusion ◽  
Permeability Studies

The study of drugs diffusion through different biological membranes constitutes an essential step in the development of new pharmaceuticals. In this study, the method based on the monolayer cell culture of CHO-K1 cells has been developed in order to emulate the epithelial cells barrier in permeability studies by laser interferometry. Laser interferometry was employed for the experimental analysis of nickel(II) and cobalt(II) complexes with 1-allylimidazole or their chlorides’ diffusion through eukaryotic cell monolayers. The amount (mol) of nickel(II) and cobalt(II) chlorides transported through the monolayer was greater than that of metals complexed with 1-allylimidazole by 4.34-fold and 1.45-fold, respectively, after 60 min. Thus, laser interferometry can be used for the quantitative analysis of the transport of compounds through eukaryotic cell monolayers, and the resulting parameters can be used to formulate a mathematical description of this process.

scholarly journals Making microenvironments: A look into incorporating macromolecular crowding into in vitro experiments, to generate biomimetic microenvironments which are capable of directing cell function for tissue engineering applications

2017 ◽  
Vol 8 ◽  
pp. 204173141773046 ◽  
Author(s):  
Paula Benny ◽  
Michael Raghunath
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Cell Function ◽  
Macromolecular Crowding ◽  
Matrix Composition ◽  
In Vitro Experiments ◽  
Current Cell ◽  
In Vitro Systems

Biomimetic microenvironments are key components to successful cell culture and tissue engineering in vitro. One of the most accurate biomimetic microenvironments is that made by the cells themselves. Cell-made microenvironments are most similar to the in vivo state as they are cell-specific and produced by the actual cells which reside in that specific microenvironment. However, cell-made microenvironments have been challenging to re-create in vitro due to the lack of extracellular matrix composition, volume and complexity which are required. By applying macromolecular crowding to current cell culture protocols, cell-made microenvironments, or cell-derived matrices, can be generated at significant rates in vitro. In this review, we will examine the causes and effects of macromolecular crowding and how it has been applied in several in vitro systems including tissue engineering.

Feast or famine: autophagy control and engineering in eukaryotic cell culture

2008 ◽  
Vol 19 (5) ◽  
pp. 518-526 ◽  
Author(s):  
Matthew P Zustiak ◽  
Judith K Pollack ◽  
Mark R Marten ◽  
Michael J Betenbaugh
Keyword(s):  
Cell Culture ◽  
Eukaryotic Cell

scholarly journals New IMAC media enabling purification of histidine‐tagged proteins directly from eukaryotic cell culture supernatants

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Therese Granér ◽  
Mattias Algotsson ◽  
Lars C Andersson ◽  
Maria Björner ◽  
Marianne Carlsson ◽  
...  
Keyword(s):  
Cell Culture ◽  
Eukaryotic Cell ◽  
Culture Supernatants
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