Role of structural properties of bioactive peptides in their stability during simulated gastrointestinal digestion: A systematic review

2022 ◽  
Author(s):  
Tausif Ahmed ◽  
Xiaohong Sun ◽  
Chibuike C. Udenigwe
Keyword(s):  
Systematic Review ◽  
Structural Properties ◽  
Bioactive Peptides ◽  
Gastrointestinal Digestion ◽  
Simulated Gastrointestinal Digestion

Identification of potential bioactive peptides generated by simulated gastrointestinal digestion of soybean seeds and soy milk proteins

2015 ◽  
Vol 44 ◽  
pp. 205-213 ◽  
Author(s):  
Anna Laura Capriotti ◽  
Giuseppe Caruso ◽  
Chiara Cavaliere ◽  
Roberto Samperi ◽  
Salvatore Ventura ◽  
...  
Keyword(s):  
Bioactive Peptides ◽  
Milk Proteins ◽  
Soybean Seeds ◽  
Soy Milk ◽  
Gastrointestinal Digestion ◽  
Simulated Gastrointestinal Digestion

scholarly journals Pepsin Hydrolysis of Orange By-Products for the Production of Bioactive Peptides with Gastrointestinal Resistant Properties

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 679
Author(s):  
Seyadeh Narges Mazloomi ◽  
Alireza Sadeghi Mahoonak ◽  
Leticia Mora ◽  
Mohammad Ghorbani ◽  
Gholamreza Houshmand ◽  
...  
Keyword(s):  
Biological Activity ◽  
Bioactive Peptides ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Size Exclusion ◽  
Gastrointestinal Digestion ◽  
Hydrolysis Time ◽  
Antioxidant Power ◽  
Simulated Gastrointestinal Digestion

Recently, the use of bioactive compounds in improving human health has received more attention. The aim of the present study was to hydrolyze orange seed proteins using pepsin enzyme to obtain bioactive peptides as well as to study the stability of such activity after simulated gastrointestinal digestion conditions. The method was optimized using different enzyme concentrations from 1% to 3%, hydrolysis times between 2 and 5 h, and an optimal temperature of 33 °C. Biological activities including α-glucosidase inhibition, α-amylase inhibition, Angiotensin I-Converting Enzyme (ACEI) inhibition, ferric reducing antioxidant power, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were evaluated. According to the results, a significant higher value of the biological activity (p < 0.05) was observed using an enzyme ratio of 0.03 E/S and hydrolysis time of 3.5 h. After size-exclusion chromatography separation, fractions 45–49 and 50–54 showed the highest biological roles such as antioxidant, ACEI inhibitory, and hypoglycemic. Fractions with the highest biological activity were purified using RP-HPLC and analyzed using nano-liquid chromatography and mass spectrometry. The results obtained after simulated gastrointestinal digestion indicated that peptide fractions obtained after chromatographic separation significantly maintain their activity.

scholarly journals Comparative Peptidomics Analysis of Fermented Milk by Lactobacillus delbrueckii ssp. Bulgaricus and Lactobacillus delbrueckii ssp. Lactis

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3028
Author(s):  
Hongji Ye ◽  
Xinyi Zhang ◽  
Yang Jiang ◽  
Min Guo ◽  
Xiaoming Liu ◽  
...  
Keyword(s):  
Bioactive Peptides ◽  
Fermented Milk ◽  
Lactobacillus Delbrueckii ◽  
Gastrointestinal Digestion ◽  
Peptide Profile ◽  
Simulated Gastrointestinal Digestion

Few studies have investigated the peptidomics of fermented milk by Lactobacillus delbrueckii. The aim of the present study was to interpret the peptidomic pattern of the fermented milk by five strains of L. delbrueckii ssp. bulgaricus and ssp. lactis prior to and after the simulated gastrointestinal digestion in vitro. The results indicated variations in the peptidomics among the samples, particularly between the samples of different subspecies. The peptides originating from β-casein were abundant in the samples of ssp. bulgaricus, whereas the peptides derived from αs1-casein and αs2-casein were more likely to dominate in those of ssp. lactis. For β-casein, the strains of ssp. bulgaricus displayed extensive hydrolysis in the regions of (73–97), (100–120), and (130–209), whereas ssp. lactis mainly focused on (160–209). The digestion appears to reduce the variations of the peptidomics profile in general. Among the five strains, L. delbrueckii ssp. bulgaricus DQHXNS8L6 was the most efficient in the generation of bioactive peptides prior to and after digestion. This research provided an approach for evaluating the peptide profile of the strains during fermentation and digestion.

Effect of cooking and simulated gastrointestinal digestion on the activity of generated bioactive peptides in aged beef meat

2017 ◽  
Vol 8 (12) ◽  
pp. 4347-4355 ◽  
Author(s):  
Leticia Mora ◽  
Tomás Bolumar ◽  
Alejandro Heres ◽  
Fidel Toldrá
Keyword(s):  
Storage Time ◽  
Bioactive Peptides ◽  
Meat Industry ◽  
Gastrointestinal Digestion ◽  
Simulated Gastrointestinal Digestion ◽  
Beef Meat

Ageing is widely used in the meat industry to improve tenderness mainly as a result of the breakdown of muscular proteins through the action of endopeptidases during storage time.

scholarly journals Bioactive Peptides from Black Bean Proteins Play a Potential Role in the Prevention of Adipogenesis (P06-119-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Cecilia Moreno Valdespino ◽  
Elvira Gonzalez de Mejia ◽  
Luis Mojica ◽  
Diego Luna-Vital ◽  
Rosa Camacho
Keyword(s):  
Common Bean ◽  
Pancreatic Lipase ◽  
Lipid Accumulation ◽  
Bioactive Peptides ◽  
Dependent Manner ◽  
Gastrointestinal Digestion ◽  
Net Charge ◽  
Black Bean ◽  
Simulated Gastrointestinal Digestion ◽  
Sds Page

Abstract Objectives Food-based products such as common bean peptides may contribute to reduce the risk of complications associated with obesity. The objective of this research was to produce and characterize peptides from common bean protein isolates and evaluate their potential to prevent adipogenesis. Methods Protein hydrolysates of raw common black bean (BBPH) were generated using a simulated gastrointestinal digestion by either pepsin/pancreatin (p/p) or a protease: alcalase. SDS-PAGE was performed for visualization of the protein profile and the generated peptides were characterized using LC-ESI-MS/MS. Bioactive peptides potential was evaluated using BIOPEP database. The antiadipogenic potential was evaluated by the pancreatic lipase (triglyceride lipases, EC 3.1.1.3) inhibition assay. Determination of lipid accumulation in 3T3-L1 adipocytes was evaluated with Oil Red lipid staining. Results Hydrolysates either with pepsin/pancreatin or alcalase showed strong inhibitory activity against pancreatic lipase with no statistical differences between them (IC50 = 3.21 and 1.23 mg/mL, respectively). Results from the SDS-PAGE indicate both BBPH had an average size of <15 kDa. It was possible to identify a higher number of peptide sequences with alcalase BBPH than using pepsin/pancreatin. Physical properties of identified peptides from pepsin/pancreatin were: SGNGGGGGASM, pI = 6, net charge = +1; SKPGGGSPVA pI = 10.1, net charge = +1; VELVGPK, pI = 6.9, net charge = +1; KPTTGKGALA, pI = 10.6, net charge = +1; the main reported bioactivities were DPP-IV and ACE inhibitors. BPPH inhibited 3T3-L1 adipocyte differentiation in a dose-dependent manner. Hydrolysates generated by simulated gastrointestinal digestion prevented lipid accumulation on adipocytes in basal conditions (IC50 = 1.08 mg/mL). Pepsin/pancreatin and alcalase BBPH promoted an important decrease in fat accumulation (45.28% and 12.08%, respectively) in comparison to non-treated adipocytes. Conclusions Common black bean peptides generated by either simulated gastrointestinal digestion or alcalase play an influential role in lipid metabolism. They could be considered a promising alternative to be integrated as functional ingredients into a food matrix. Funding Sources Scientific Projects Grant “Para Atender problemas Nacionales” 2016 No. 2081. CONACYT, and USDA-NIFA-HATCH project 1,014,457.

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