Cultured human peripheral blood mononuclear cells alter their gene expression when challenged with endocrine-disrupting chemicals

Toxicology ◽  
2013 ◽  
Vol 303 ◽  
pp. 17-24 ◽  
Author(s):  
B. Wens ◽  
P. De Boever ◽  
M. Verbeke ◽  
K. Hollanders ◽  
G. Schoeters
Keyword(s):  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Endocrine Disrupting Chemicals ◽  
Peripheral Blood Mononuclear ◽  
Endocrine Disrupting ◽  
Blood Mononuclear Cells

Gene expression in human peripheral blood mononuclear cells upon acute ischemic stroke

2008 ◽  
Vol 255 (5) ◽  
pp. 723-731 ◽  
Author(s):  
C. Grond-Ginsbach ◽  
M. Hummel ◽  
T. Wiest ◽  
S. Horstmann ◽  
K. Pfleger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Differential Gene Expression in Human Peripheral Blood Mononuclear Cells Induced by Cigarette Smoke and Its Constituents

2005 ◽  
Vol 86 (1) ◽  
pp. 200-210 ◽  
Author(s):  
Danitsja M. van Leeuwen ◽  
Ralph W. H. Gottschalk ◽  
Marcel H. van Herwijnen ◽  
Edwin J. Moonen ◽  
Jos C. S. Kleinjans ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cigarette Smoke ◽  
Differential Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Differential Gene

scholarly journals Investigation of low-dose ritonavir on human peripheral blood mononuclear cells using gene expression whole genome microarrays

Genomics ◽  
2010 ◽  
Vol 96 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Saliha Yilmaz ◽  
Marta Boffito ◽  
Sophie Collot-Teixeira ◽  
Ferruccio De Lorenzo ◽  
Laura Waters ◽  
...  
Keyword(s):  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Low Dose ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Whole Genome ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

A pathway analysis of poly(I:C)-induced global gene expression change in human peripheral blood mononuclear cells

2006 ◽  
Vol 26 (2) ◽  
pp. 125-133 ◽  
Author(s):  
C. Chris Huang ◽  
Karen E. Duffy ◽  
Lani R. San Mateo ◽  
Bernard Y. Amegadzie ◽  
Robert T. Sarisky ◽  
...  
Keyword(s):  
Gene Expression ◽  
Viral Infection ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Human Peripheral Blood ◽  
Poly I:C ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

To gain global pathway perspective of ex vivo viral infection models using human peripheral blood mononuclear cells (PBMCs), we conducted expression analysis on PBMCs of healthy donors. RNA samples were collected at 3 and 24 h after PBMCs were challenged with the Toll-like receptor-3 (TLR3) agonist polyinosinic acid-polycytidylic acid [poly(I:C)] and analyzed by internally developed cDNA microarrays and TaqMan PCR. Our results demonstrate that poly(I:C) challenge can elicit certain gene expression changes, similar to acute viral infection. Hierarchical clustering revealed distinct immediate early, early-to-late, and late gene regulation patterns. The early responses were innate immune responses that involve TLR3, the NF-κB-dependent pathway, and the IFN-stimulated pathway, whereas the late responses were mostly cell-mediated immune response that involve activation of cell adhesion, cell mobility, and phagocytosis. Overall, our results expanded the utilities of this ex vivo model, which could be used to screen molecules that can modulate viral stress-induced inflammation, in particular those mediated via TLRs.

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