Prepubertal arsenic exposure alters phosphoproteins profile, quality, and fertility of epididymal spermatozoa in sexually mature rats

Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate to the loss of sperm function during cryopreservation remains unsolved. The present study aimed to clarify this issue evaluating differential changes in the proteome of fresh and frozen-thawed pig spermatozoa retrieved from the cauda epididymis and the ejaculate of the same boars, with clear differences in cryotolerance. Spermatozoa were collected from 10 healthy, sexually mature, and fertile boars, and cryopreserved using a standard 0.5 mL-straw protocol. Total and progressive motility, viability, and mitochondria membrane potential were higher and membrane fluidity and reactive oxygen species generation lower in frozen-thawed (FT) epididymal than ejaculated spermatozoa. Quantitative proteomics of fresh and FT spermatozoa were analyzed using a LC-ESI-MS/MS-based Sequential Window Acquisition of All Theoretical Spectra approach. Cryopreservation quantitatively altered more proteins in ejaculated than cauda epididymal spermatozoa. Differential protein–protein networks highlighted a set of proteins quantitatively altered in ejaculated spermatozoa, directly involved in mitochondrial functionality which would explain why ejaculated spermatozoa deteriorate during cryopreservation.

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Ultrastructure of the basal body apparatus in epidermal cells of a sponge larva (Aplysilla SP: Demospongiae)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012504x ◽

1992 ◽

Vol 50 (1) ◽

pp. 928-929

Author(s):

R.L. Pinto ◽

R.M. Woollacott

Keyword(s):

Sodium Bicarbonate ◽

Epoxy Resin ◽

Basal Body ◽

Phosphate Buffer ◽

Similar Data ◽

Basal Apparatus ◽

Striated Rootlet ◽

The Common ◽

Basal Foot ◽

Sexually Mature

The basal body and its associated rootlet are the organelles responsible for anchoring the flagellum or cilium in the cytoplasm. Structurally, the common denominators of the basal apparatus are the basal body, a basal foot from which microtubules or microfilaments emanate, and a striated rootlet. A study of the basal apparatus from cells of the epidermis of a sponge larva was initiated to provide a comparison with similar data on adult sponges.Sexually mature colonies of Aplysillasp were collected from Keehi Lagoon Marina, Honolulu, Hawaii. Larvae were fixed in 2.5% glutaraldehyde and 0.14 M NaCl in 0.2 M Millonig’s phosphate buffer (pH 7.4). Specimens were postfixed in 1% OsO4 in 1.25% sodium bicarbonate (pH 7.2) and embedded in epoxy resin. The larva ofAplysilla sp was previously described (as Dendrilla cactus) based on live observations and SEM by Woollacott and Hadfield.

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37. Use of Portable X-Ray Fluorescence (PXRF) in Monitoring Arsenic Exposure During the Preventive Maintenance Task in the Microelectronic Industry

10.3320/1.2758624 ◽

2005 ◽

Author(s):

Y. Hwang ◽

W. Chu ◽

T. Shi ◽

D. Taung ◽

Y. Hsiou ◽

...

Keyword(s):

Preventive Maintenance ◽

Arsenic Exposure ◽

X Ray

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Fertility i does inseminated with epididymal spermatozoa

Reproduction ◽

10.1530/jrf.0.0140346 ◽

1967 ◽

Vol 14 (2) ◽

pp. 346-347 ◽

Cited By ~ 3

Author(s):

M. Orgebin-Crist

Keyword(s):

Epididymal Spermatozoa

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The chemical composition and meat yield of sexually mature cuttlefish (Sepia officinalis)

Journal of FisheriesSciences com ◽

10.3153/jfscom.2012013 ◽

2012 ◽

Author(s):

Yeşim Özoğul

Keyword(s):

Chemical Composition ◽

Sepia Officinalis ◽

Meat Yield ◽

Sexually Mature

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Assessment of Pomegranate Juice as Antioxidant in Extender on Cauda Epididymis Spermatozoal Quality of Buck at Refrigerated Temperature

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.15.2.7 ◽

2019 ◽

Vol 15 (02) ◽

pp. 26-29

Author(s):

Amarjeet Amarjeet ◽

C T Khasatiya ◽

L Chaudhary

Keyword(s):

Detrimental Effect ◽

Egg Yolk ◽

Antioxidant Effect ◽

Pomegranate Juice ◽

Antioxidant Additive ◽

Epididymal Spermatozoa ◽

The Dead

The present investigation was carried out to study the refrigeration preservation of the cauda epididymal retrieved spermatozoa of buck in Tris egg yolk citrate (TEYC) dilutor containing pomegranate juice as antioxidant additive. The retrieved cauda epididymal spermatozoa extended in TEYC dilutor were studied in five groups by adding different concentration of pomegranate juice as additive (0% as control T1 group and 5%, 10%, 15% and 20% as treatment T2, T3, T4 and T5 groups, respectively) and storing at refrigerated temperature up to 48 hr. The results showed that the control extender had the least dead, abnormal and HOS non-reacted sperm percent among all treatments tested and that with increasing the pomegranate juice concentration in dilutor, the percentage of the dead, abnormal and HOST non-reacted spermatozoa increased significantly. The same trend was observed at all 12 hourly storage intervals indicating its detrimental effect on epididymal sperms of bucks at refrigeration temperature. The dead, abnormal, and HOST non-reacted sperm were significantly and positively interrelated with each other (r = 0.53-0.83). It was concluded that the inclusion of pomegranate juice in TEYC dilutor did not show any beneficial/antioxidant effect on epididymal sperms of buck in fresh or refrigerated semen and in fact all the levels of pomegranate juice (5% to 20%) were detrimental to cauda epididymal spermatozoa of a buck.

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Effect of Tomato Juice in Tris-Yolk-Citrate Extender onRefrigeration Preservation of Cauda Epididymal Spermatozoa of Buck

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.15.1.17 ◽

2019 ◽

Vol 15 (01) ◽

pp. 71-74

Author(s):

L M Chaudhary ◽

C T Khasatiya ◽

Amarjeet Amarjeet ◽

A B Yede

Keyword(s):

Adverse Effect ◽

Egg Yolk ◽

Physical Characteristics ◽

Tomato Juice ◽

The Mean ◽

Epididymal Spermatozoa ◽

Sperm Traits

The study was carried out on the preservation of epididymal spermatozoa of buck at refrigerated temperature without and with tomato juice as a supplement in Tris egg yolk citrate extender. The eight pairs of testicles including epididymis (total 16) from slaughtered bucks were collected within 2–4 hours of their slaughter. Sperms collected from cauda epididymis were preserved at refrigerated temperature up to 48 hours in tris egg yolk citrate extender at 300 million sperm/mL with different concentration of tomato juice (0%, 4%, 6%, 8%, and 10%) and the physical characteristics of spermatozoa were assessed to know the effect of tomato juice (Tj). The mean dead, abnormal and HOST non-reacted spermatozoa increased significantly (p less than0.05) at every 12-hour intervals of preservation in the dilutor without and with different concentration of tomato juice. Tomato juice exerted an adverse effect on physical characteristics of sperm during refrigeration preservation. All the three sperm traits studied however revealed significant (p less thaN 0.01) positive interrelationships with correlations of 0.31 to 0.72.

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