Modulation of hepatic gene expression and DNA methylation in furan treated Sprague–Dawley rats

Although soy foods have been recognized as an excellent source of protein, there have been recent concerns regarding potential adverse effects of isoflavone phytochemicals found in soy products, which are known to bind and activate estrogen receptors. Here, we used global hepatic gene expression profiles in ovariectomized female Sprague–Dawley rats treated with 17β-estradiol (E2) or fed with soy protein isolate (SPI) as a means of estimating potential estrogenicity of SPI. Female Sprague–Dawley rats were fed AIN-93G diets containing casein (CAS) or SPI starting at postnatal day (PND) 30. Rats were ovariectomized on PND 50 and infused with E2 or vehicle in osmotic pumps for 14 d. Microarray analysis was performed on liver using Affymetrix GeneChip Rat 230 2.0. Serum E2 levels were within normal ranges for the rat and SPI feeding did not increase uterine wet weight in the absence or presence of E2. SPI feeding altered (P<0.05, ≥±1.5-fold) the expression of 82 genes, while E2 treatment altered 892 genes. Moreover, only 4% of E2-affected genes were also modulated by SPI, including some whose expression was reversed by SPI feeding. The interaction between E2 and SPI uniquely modulated the expression profile of 225 genes including the reduction of those involved in fatty acid biosynthesis or glucocorticoid signaling and an induction of those involved in cholesterol metabolism. The different hepatic gene signatures produced by SPI feeding compared with E2 and the lack of increase in uterine wet weight in rats fed with SPI suggest that SPI is not estrogenic in these tissues.

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Global DNA Methylation Patterns and Gene Expression Associated with Obesity-Susceptibility in Offspring of Pregnant Sprague-Dawley Rats Exposed to BDE-47 and BDE-209

Korean Journal of Clinical Laboratory Science ◽

10.15324/kjcls.2017.49.1.28 ◽

2017 ◽

Vol 49 (1) ◽

pp. 28-39 ◽

Cited By ~ 1

Author(s):

Byeong-Min Park ◽

Ok-Jin Yoon ◽

Do-Hoon Lee

Keyword(s):

Gene Expression ◽

Dna Methylation ◽

Global Dna Methylation ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Obesity Susceptibility ◽

Methylation Patterns ◽

Bde 209

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Febuxostat Attenuates Renal Damage besides Exerting Hypouricemic Effect in Streptozotocin-Induced Diabetic Rats

International Journal of Nephrology ◽

10.1155/2017/2739539 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Jianmin Ran ◽

Gang Xu ◽

Huixuan Ma ◽

Hailing Xu ◽

Yan Liu ◽

...

Keyword(s):

Gene Expression ◽

Uric Acid ◽

Renal Damage ◽

Diabetic Rats ◽

Urinary Albumin ◽

Hepatic Gene Expression ◽

Sprague Dawley ◽

Urea Nitrogen ◽

Sprague Dawley Rats ◽

Rat Livers

Aim. In this study, we aimed to investigate the effects of febuxostat, a novel inhibitor of xanthine oxidase (XO), on renal damage in streptozotocin- (STZ-) induced diabetic rats. Methods. Diabetes was induced by the intraperitoneal injection of STZ in male Sprague-Dawley rats. Sham-injected rats served as controls. The control and diabetic rats were treated with and without febuxostat for 8 weeks, respectively. Fasting blood and 24-h urine samples were collected every 4 weeks. Rat livers were extracted for detecting gene expression, content, and bioactivity of XO. Results. Diabetic rats showed significantly increased serum uric acid (SUA), serum creatinine (SCr), and urea nitrogen (BUN) levels. Daily urinary albumin (UAE), uric acid (UUA), and creatinine (UCr) excretion were also significantly increased in these rats. In diabetic rats, at week 8, febuxostat decreased SUA by 18.9%, while UAA was increased by 52.0%. However, UCr and urinary urea nitrogen (UUN) levels remained unchanged, while SCr and BUN levels decreased by >30% in these rats. Although hepatic gene expression, content, and activity of XO increased significantly in diabetic rats, febuxostat only slightly decreased its content. Conclusions. Febuxostat significantly attenuated renal damage in STZ-induced diabetic rats in addition to exerting hypouricemic effect.

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Local mechanisms for acupoint sensitization in gall bladder meridian of foot shaoyang-a gene chip study

Acupuncture & Electro-Therapeutics Research ◽

10.3727/036012919x15650315071898 ◽

2019 ◽

Vol 44 (2) ◽

pp. 77-87

Author(s):

Koichi Ishida ◽

Liyue Qin ◽

Ting Wang ◽

Ying Lei ◽

Weiwei Hu ◽

...

Keyword(s):

Gene Expression ◽

Gall Bladder ◽

Interleukin 1 ◽

Gene Chip ◽

Molecular Evidence ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Bladder Meridian ◽

Integrin Linked Kinase ◽

Necrosis Factor

Acupuncture manipulations are clinically important to traditional Chinese medicine, yet the biological mechanisms have not been fully understood. This study aimed to investigate continuous stimulation-induced gene expression changes at stimulated and non-stimulated adjacent acupoints in the same meridian. Catgut embedding into acupoint (CEP) was conducted at acupoint Yanglingquan (gall bladder meridian of foot-shaoyang 34, GB34) of Sprague Dawley rats once or continuously for eight weeks, and gene expression changes at GB34 were assessed by gene chip array analysis 72 h after the last CEP treatment. A total of 688 genes exhibited opposite changes in expression between the two treatments, and 1,336 genes were regulated only by the eight-week CEP treatment. Ingenuity Pathway Analysis revealed that among these differentially regulated genes by one-time and eight-week CEP treatment, insulin-like growth factor-1 pathway and integrin-linked kinase pathway, and Wnt/~ catenin signaling pathway match the observed gene changes to predicted up/down regulation patterns. Upstream analysis further predicted six molecules, namely, tumor necrosis factor, interleukin 1~, interleukin la, kallikrein-related peptidase 5, protein kinase Ca, and catenin ~1. On the other hand, continuous eight-week CEP stimulation at acupoint Xuanzhong (GB39) caused similar changes in the expression of 32 genes at acupoints GB34 and Fengshi (GB31) on the same meridian. Taken together, our results provide the first molecular evidence for the local acupoints' mechanisms for acupoint sensitization theory, and implicate the existence of signaling pathways, either direct or indirect, between acupoints within the meridian GB.

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DMBA Induction Increases H-Ras Gene Expression and Decreases CD8 Count in Sprague Dawley Rats

Proceedings of the 2019 6th International Conference on Biomedical and Bioinformatics Engineering ◽

10.1145/3375923.3375942 ◽

2019 ◽

Author(s):

Titiek Hidayati ◽

Indrayanti ◽

Sagiran

Keyword(s):

Gene Expression ◽

Sprague Dawley ◽

Ras Gene ◽

Sprague Dawley Rats

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Differential Effects of Refeeding on Melanocortin-Responsive Neurons in the Hypothalamic Paraventricular Nucleus

Endocrinology ◽

10.1210/en.2008-0411 ◽

2008 ◽

Vol 149 (9) ◽

pp. 4329-4335 ◽

Cited By ~ 17

Author(s):

Edith Sánchez ◽

Praful S. Singru ◽

Runa Acharya ◽

Monica Bodria ◽

Csaba Fekete ◽

...

Keyword(s):

Gene Expression ◽

Paraventricular Nucleus ◽

Hypothalamic Paraventricular Nucleus ◽

Mrna Levels ◽

Sprague Dawley ◽

Early Action ◽

Sprague Dawley Rats ◽

Melanocortin Signaling ◽

Agouti Related Protein ◽

Serum Tsh

To explore the effect of refeeding on recovery of TRH gene expression in the hypothalamic paraventricular nucleus (PVN) and its correlation with the feeding-related neuropeptides in the arcuate nucleus (ARC), c-fos immunoreactivity (IR) in the PVN and ARC 2 h after refeeding and hypothalamic TRH, neuropeptide Y (NPY) and agouti-related protein (AGRP) mRNA levels 4, 12, and 24 h after refeeding were studied in Sprague-Dawley rats subjected to prolonged fasting. Despite rapid reactivation of proopiomelanocortin neurons by refeeding as demonstrated by c-fos IR in ARC α-MSH-IR neurons and ventral parvocellular subdivision PVN neurons, c-fos IR was present in only 9.7 ± 1.1% hypophysiotropic TRH neurons. Serum TSH levels remained suppressed 4 and 12 h after the start of refeeding, returning to fed levels after 24 h. Fasting reduced TRH mRNA compared with fed animals, and similar to TSH, remained suppressed at 4 and 12 h after refeeding, returning toward normal at 24 h. AGRP and NPY gene expression in the ARC were markedly elevated in fasting rats, AGRP mRNA returning to baseline levels 12 h after refeeding and NPY mRNA remaining persistently elevated even at 24 h. These data raise the possibility that refeeding-induced activation of melanocortin signaling exerts differential actions on its target neurons in the PVN, an early action directed at neurons that may be involved in satiety, and a later action on hypophysiotropic TRH neurons involved in energy expenditure, potentially mediated by sustained elevations in AGRP and NPY. This response may be an important homeostatic mechanism to allow replenishment of depleted energy stores associated with fasting.

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