Advances in sulfur mustard-induced DNA adducts: Characterization and detection

2021 ◽  
Vol 344 ◽  
pp. 46-57
Author(s):  
Xi Cheng ◽  
Changcai Liu ◽  
Yang Yang ◽  
Longhui Liang ◽  
Bo Chen ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Sulfur Mustard

scholarly journals Alkylated epidermal creatine kinase as a biomarker for sulfur mustard exposure: comparison to adducts of albumin and DNA in an in vivo rat study

2021 ◽  
Author(s):  
Dirk Steinritz ◽  
Robin Lüling ◽  
Markus Siegert ◽  
Julia Herbert ◽  
Harald Mückter ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Dna Adducts ◽  
Sulfur Mustard ◽  
Ex Vivo ◽  
Immunomagnetic Separation ◽  
Chemical Warfare Agent ◽  
Cysteine Residue ◽  
Islamic State ◽  
Rat Skin

AbstractSulfur mustard (SM) is a chemical warfare agent which use is banned under international law and that has been used recently in Northern Iraq and Syria by the so-called Islamic State. SM induces the alkylation of endogenous proteins like albumin and hemoglobin thus forming covalent adducts that are targeted by bioanalytical methods for the verification of systemic poisoning. We herein report a novel biomarker, namely creatine kinase (CK) B-type, suitable as a local biomarker for SM exposure on the skin. Human and rat skin were proven to contain CK B-type by Western blot analysis. Following exposure to SM ex vivo, the CK-adduct was extracted from homogenates by immunomagnetic separation and proteolyzed afterwards. The cysteine residue Cys282 was found to be alkylated by the SM-specific hydroxyethylthioethyl (HETE)-moiety detected as the biomarker tetrapeptide TC(-HETE)PS. A selective and sensitive micro liquid chromatography-electrospray ionization high-resolution tandem-mass spectrometry (µLC-ESI MS/HRMS) method was developed to monitor local CK-adducts in an in vivo study with rats percutaneously exposed to SM. CK-adduct formation was compared to already established DNA- and systemic albumin biomarkers. CK- and DNA-adducts were successfully detected in biopsies of exposed rat skin as well as albumin-adducts in plasma. Relative biomarker concentrations make the CK-adduct highly appropriate as a local dermal biomarker. In summary, CK or rather Cys282 in CK B-type was identified as a new, additional dermal target of local SM exposures. To our knowledge, it is also the first time that HETE-albumin adducts, and HETE-DNA adducts were monitored simultaneously in an in vivo animal study.

Temporal and spatial features of the formation of DNA adducts in sulfur mustard-exposed skin

2013 ◽  
Vol 273 (3) ◽  
pp. 644-650 ◽  
Author(s):  
Mohamed Batal ◽  
Isabelle Boudry ◽  
Stéphane Mouret ◽  
Julien Wartelle ◽  
Sandy Emorine ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Sulfur Mustard ◽  
Exposed Skin ◽  
Spatial Features ◽  
Temporal And Spatial

Modified immunoslotblot assay to detect hemi and sulfur mustard DNA adducts

2013 ◽  
Vol 206 (3) ◽  
pp. 523-528 ◽  
Author(s):  
Kai Kehe ◽  
Verena Schrettl ◽  
Horst Thiermann ◽  
Dirk Steinritz
Keyword(s):  
Dna Adducts ◽  
Sulfur Mustard

Synthesis, Characterization, and Identification of New in Vitro Covalent DNA Adducts of Divinyl Sulfone, an Oxidative Metabolite of Sulfur Mustard

2017 ◽  
Vol 30 (10) ◽  
pp. 1874-1882 ◽  
Author(s):  
Shanshan Lv ◽  
Yajiao Zhang ◽  
Bin Xu ◽  
Hua Xu ◽  
Yumei Zhao ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Sulfur Mustard ◽  
Divinyl Sulfone

Analysis of Different Fates of DNA Adducts in Adipocytes Post-sulfur Mustard Exposurein Vitroandin VivoUsing a Simultaneous UPLC-MS/MS Quantification Method

2015 ◽  
Vol 28 (6) ◽  
pp. 1224-1233 ◽  
Author(s):  
Peng Wang ◽  
Yajiao Zhang ◽  
Jia Chen ◽  
Lei Guo ◽  
Bin Xu ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Sulfur Mustard ◽  
Quantification Method

Distribution of DNA Adducts and Corresponding Tissue Damage of Sprague–Dawley Rats with Percutaneous Exposure to Sulfur Mustard

2015 ◽  
Vol 28 (3) ◽  
pp. 532-540 ◽  
Author(s):  
Lijun Yue ◽  
Yajiao Zhang ◽  
Jia Chen ◽  
Zengming Zhao ◽  
Qin Liu ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Tissue Damage ◽  
Sulfur Mustard ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Percutaneous Exposure

Toxicokinetics of Sulfur Mustard and its Dna-Adducts in the Hairless Guinea Pig

1998 ◽  
Vol 21 (sup1) ◽  
pp. 131-147 ◽  
Author(s):  
Jan P. Langenberg ◽  
Govert P. van der Schans ◽  
Helma E.T. Spruit ◽  
Willem C. Kuijpers ◽  
Roos H. Mars-Groenendijk ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Dna Adducts ◽  
Sulfur Mustard ◽  
Hairless Guinea Pig
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