Screening of specific binding peptides using phage-display techniques and their biosensing applications

2021 ◽  
Vol 137 ◽  
pp. 116229
Author(s):  
Ping Xu ◽  
Subhadeep Ghosh ◽  
Anam Rana Gul ◽  
Jigna R. Bhamore ◽  
Jong Pil Park ◽  
...  
Keyword(s):  
Phage Display ◽  
Specific Binding ◽  
Binding Peptides

Identification of Indium Tin Oxide Nanoparticle-Binding Peptides via Phage Display and Biopanning Under Various Buffer Conditions

2020 ◽  
Vol 27 (6) ◽  
pp. 557-566
Author(s):  
Hikaru Nakazawa ◽  
Mitsuo Umetsu ◽  
Tatsuya Hirose ◽  
Takamitsu Hattori ◽  
Izumi Kumagai
Keyword(s):  
Phage Display ◽  
Tin Oxide ◽  
Indium Tin Oxide ◽  
Specific Binding ◽  
Oxide Nanoparticles ◽  
Binding Interface ◽  
Binding Peptides ◽  
Tin Oxide Nanoparticles ◽  
Sensor Proteins ◽  
Indium Tin Oxide Nanoparticles

Background: By recent advances in phage-display approaches, many oligopeptides exhibiting binding affinities for metal oxides have been identified. Indium tin oxide is one of the most widely used conductive oxides, because it has a large band gap of 3.7–4.0 eV. In recent years, there have been reports about several ITO-based biosensors. Development of an ITO binding interface for the clustering of sensor proteins without complex bioconjugates is required. Objective: In this article, we aimed to identify peptides that bind to indium tin oxide nanoparticles via different binding mechanisms. Methods: Indium tin oxide nanoparticles binding peptide ware selected using phage display and biopanning against indium tin oxide, under five different buffer conditions and these peptides characterized about binding affinity and specificity. Results: Three types of indium tin oxide nanoparticles-binding peptides were selected from 10 types of peptide candidates identified in phage display and biopanning. These included ITOBP8, which had an acidic isoelectric point, and was identified when a buffer containing guanidine was used, and ITOBP6 and ITOBP7, which contained a His-His-Lys sequence at their N-termini, and were identified when a highly concentrated phosphate elution buffer with a low ionic strength was used. Among these peptides, ITOBP6 exhibited the strongest indium tin oxide nanoparticlesbinding affinity (dissociation constant, 585 nmol/L; amount of protein bound at saturation, 17.5 nmol/m 2 - particles). Conclusion: These results indicate that peptides with specific binding properties can be obtained through careful selection of the buffer conditions in which the biopanning procedure is performed.

Colorimetric detection of creatinine using its specific binding peptides and gold nanoparticles

2020 ◽  
Vol 44 (37) ◽  
pp. 15828-15835
Author(s):  
Shuaihui Feng ◽  
Rongjia Shi ◽  
Ping Xu ◽  
Jigna R. Bhamore ◽  
Jyotiranjan Bal ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Phage Display ◽  
Detection Method ◽  
Specific Binding ◽  
Colorimetric Detection ◽  
Selective Detection ◽  
Phage Display Technology ◽  
Display Technology ◽  
Binding Peptides ◽  
Peptide Probes

A new selective detection method was developed for creatinine by combining gold nanoparticles (GNPs) and peptide probes which were screened and selected using phage-display technology.

Simplified Expression and Production of Small Metal Binding Peptides

2017 ◽  
Vol 262 ◽  
pp. 447-451 ◽  
Author(s):  
Robert Braun ◽  
Sabine Matys ◽  
Nora Schönberger ◽  
Franziska L. Lederer ◽  
Katrin Pollmann
Keyword(s):  
Pharmaceutical Industry ◽  
Phage Display ◽  
Metal Binding ◽  
State Of The Art ◽  
Specific Binding ◽  
Expression System ◽  
Cloning And Expression ◽  
Biotechnological Applications ◽  
Metal Binding Peptides ◽  
Binding Peptides

Phage display for discovery of specific binding peptides is nowadays widely used in the pharmaceutical industry and in many biotechnological applications. Using state-of-the-art cloning techniques we developed an easy-to-use cloning and expression system, allowing the fast production of identified peptides while avoiding proteolysis.

scholarly journals Screening a Phage Display Library for Two Novel OmpU-Binding Peptides with Adhesion Antagonistic Activity against Vibrio mimicus

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0165092 ◽  
Author(s):  
Lifang Qi ◽  
Yan Liu ◽  
Huizhu Tao ◽  
Ning Xiao ◽  
Jinnian Li ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Display Library ◽  
Antagonistic Activity ◽  
Vibrio Mimicus ◽  
Binding Peptides

Physical elution in phage display selection of inorganic-binding peptides

2009 ◽  
Vol 29 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Senem Donatan ◽  
Hilal Yazici ◽  
Hakan Bermek ◽  
Mehmet Sarikaya ◽  
Candan Tamerler ◽  
...  
Keyword(s):  
Phage Display ◽  
Binding Peptides ◽  
Selection Of

Muscle binding peptides found by phage display as delivery agent for antisense oligonucleotides

2008 ◽  
Vol 132 (3) ◽  
pp. e3-e5 ◽  
Author(s):  
H. Heemskerk ◽  
B. Aguilera ◽  
A. Janson ◽  
K.H. Pang ◽  
G.-J. van Ommen ◽  
...  
Keyword(s):  
Phage Display ◽  
Antisense Oligonucleotides ◽  
Binding Peptides

scholarly journals Multidrug-resistance drug-binding peptides generated by using a phage display library

1998 ◽  
Vol 251 (1-2) ◽  
pp. 155-163 ◽  
Author(s):  
Mikhail Popkov ◽  
Isabelle Lussier ◽  
Viatcheslav Medvedkine ◽  
Pierre-Olivier Esteve ◽  
Valery Alakhov ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Phage Display ◽  
Phage Display Library ◽  
Drug Binding ◽  
Binding Peptides

Application of Phage Biotechnology in Nanobiotechnology

2015 ◽  
pp. 36-48
Author(s):  
Rana Singleton ◽  
Carrie Sanders ◽  
Alain B. Waffo
Keyword(s):  
Phage Display ◽  
The Novel ◽  
Chemical Surface ◽  
Replication Systems ◽  
Versatile Tool ◽  
Amplification Process ◽  
Binding Peptides ◽  
Peptide Display ◽  
Display Systems ◽  
Rna Phage

To date, the phage display system has enabled the discovery of material binding peptides. Diversity and functionality of these peptides could be improved using RNA-based display systems instead of the conventional DNA-based ones. RNA phage replication systems possess unique features that make them a versatile tool for any combinatory approach and evolutionary application. Phage display was used to monitor the chemical surface properties and to initiate nanoparticle assembly. Novel bio-panning was recently used in RNA-based display to screen new functionality without acidic elution used in other conventional DNA phage display systems. Therefore, Hybrid RNA phages would be a perfect platform for attachment and exploration of nanoparticles. In this chapter, the authors present an overview on research conducted on these cross fields and areas. They not only focus on the novel selection and amplification process but also on the importance of RNA phage and its peptide display as tools for preventing nanoparticle aggregation.

Corrigendum to “Lipopolysaccaride-binding peptides obtained by phage display method” [J. Microbiol. Methods 82 (2010) 54–58]

2010 ◽  
Vol 83 (1) ◽  
pp. 87
Author(s):  
Megumi Matsumoto ◽  
Yoshinobu Horiuchi ◽  
Akihiko Yamamoto ◽  
Masaki Ochiai ◽  
Makoto Niwa ◽  
...  
Keyword(s):  
Phage Display ◽  
Binding Peptides
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