Implementation of an electronic identification system in the setting of perioperative autologous cell salvage transfusion: Experience at a university hospital

Author(s):  
Yoshiaki Furuta ◽  
Yuki Nakamura ◽  
Miho Tokida ◽  
Kayoko Ichikawa ◽  
Hisako Okada ◽  
...  
2020 ◽  
Author(s):  
Sibylle Kietaibl

Background and Objective: Prerequisite for oxygen transport to the tissue is the ability of erythrocytes to change from a discoid into an elongated form in order to pass microvessels. Cell salvage is highly recommended to decrease blood loss and to avoid allogeneic transfusions in perioperative bleeding. The purpose of our study was to assess deformability of erythrocytes at re-transfusion. Materials and Methods: After ethics committee approval and informed consent, blood was withdrawn from the autotransfusion system (Xtra, Sorin, Germany) of 24 patients undergoing joint arthroplasty 6 hours after cell salvage initiation. Deformability curves, elongation indices (EI) obtained at increasing shear stress (SS) were assessed by using Laser Optical Rotational Red Cell Analysis (LORRCA). Results: Erythrocytes showed the typical sigmoid EI/SS curve with a mean maximum elongation index (EImax) of 0.593 ± 0.034 and mean half-maximal deformation (SS ½) of 1.186 ± 0.387 Pa. Irregular curve shapes and high variability in EI occurred at shear stress < 3 Pa. Recalculation of outcome parameters resulted in higher values (EImax 0.610 ± 0.035; SS ½ 1.562 ± 0.346 Pa). Conclusion: Erythrocytes after autologous cell salvage are not stiff but elongate in response to shear stress. Erythrocytes showed the typical EI/SS-curve of human red cells, which indicates that the cells are able to uptake the hydrodynamic forces that act on them during the measurement. Further research is needed to investigate the effect of cell salvage processing on erythrocytes from patients with pre-existing hematological disorders.


Vox Sanguinis ◽  
2018 ◽  
Vol 113 (6) ◽  
pp. 562-568 ◽  
Author(s):  
G. Enten ◽  
P. Dalvi ◽  
N. Martini ◽  
K. Kausch ◽  
A. Gray ◽  
...  

Surgeries ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 84-91
Author(s):  
Maximilian Kutschera ◽  
Agnes Pischlaeger ◽  
Larissa Sztulman ◽  
Sibylle Kietaibl ◽  
Andreas Spittler

(1) Background: Cell salvage is highly recommended in orthopedic surgery to avoid allogeneic transfusions. Preparational steps during cell salvage may induce extracellular vesicle (EV) formation with potential thrombogenic activity. The purpose of our study was to assess the appearance of EVs at retransfusion. (2) Methods: After ethics committee approval and informed consent, blood was withdrawn from the autotransfusion system (Xtra, Sorin, Germany) of 23 patients undergoing joint arthroplasty. EVs were assessed by flow cytometry in two times centrifugated samples. EVs were stained with specific antibodies against cellular origins from platelets (CD41), myeloid cells (CD15), monocytes (CD14), and erythrocytes (CD235a). The measured events/µL in the flow cytometer were corrected to the number of EVs in the retransfusate. (3) Results: We measured low event rates of EVs from platelets and myeloid origin (<1 event/µL) and from monocytic origin (<2 events/µL). Mean event rates of 17,042 events/µL (range 12–81,164 events/µL) were found for EVs from red blood cells. (4) Conclusion: Retransfusate contains negligible amounts of potentially thrombogenic EVs from platelet and monocytic origin. Frequent EVs from erythrocytes may indicate red blood cell destruction and/or activation during autologous cell salvage. Further research is needed to investigate the clinical relevance of EVs from salvaged red blood cells.


2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S160-S161
Author(s):  
J M Petersen ◽  
D Jhala

Abstract Introduction/Objective Blood conservation techniques such as perioperative autologous cell salvage (PACS) can reduce patient usage and exposure to allogeneic blood products during surgery. As all blood transfusions carry risk and cost, the avoidance of transfusion through PACS represents an opportunity to reduce risk of allogeneic transfusion related reactions or complications and reduce costs from blood product utilization. Although the benefits of PACS has been documented in the literature, quality assurance demonstrations of the blood volume returned to veteran patients undergoing surgeries with a high likelihood of significant blood loss are sparse; thus, we present the quality assurance study of PACS usage in a VAMC. Methods Quality assurance documentation from the American Red Cross (ARC) that provides autologous cell salvage services at the VAMC were examined from February 2017 to August 2018, for a total of 17 months examined. The procedure, estimated blood loss, and volume returned was documented as part of a quality assurance/improvement project. The volume returned was compared to the typical RBC volume (~250 mL) within a packed red blood cell volume (~350 mL). Results A total of 44 procedures took place corresponding usage of PACS. Of these 44 procedures, 15 had no estimated blood loss, and an additional 10 had too little estimated blood loss (&lt;250 mL) for blood volume to be returned. Among the procedures with blood loss, an average of 864 mL of blood was lost (1230 mL when the low blood loss cases are excluded) with an average volume returned of 511 mL or the approximate equivalent of 2 units of RBCs when utilized. Two cases with significant estimated blood loss (5400 mL and 4850 mL) had 2250 mL and 2125 mL or about the equivalent of 9 RBC units each. The total volume returned to all patients was 9700 mL, or the equivalent RBC volume of 38-39 RBC units. Conclusion PACS is a procedure that can rapidly provide large quantities of autologous blood in surgeries with significant risk of bleeding. Blood salvage avoids or reduces the risks of allogeneic blood transfusion and reduces cost to the transfusion service as the autologous blood loss would not need to be replaced with allogeneic blood transfusions. PACS in the VAMC returned significant blood volumes to patients undergoing procedures with high risk of blood loss, therefore reducing allogeneic blood transfusion requirements in the veteran population.


2021 ◽  
Author(s):  
Yuki Nakamura ◽  
Yoshiaki Furuta ◽  
Miho Tokida ◽  
Kayoko Ichikawa ◽  
Mineko Shirahata ◽  
...  

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Danyar Hameed M. Amin ◽  
Emrah Guler ◽  
Buket Baddal

Abstract Objective Panton-Valentine leukocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) is a healthcare problem worldwide. There are no reports on the virulence characteristics of MRSA in Northern Cyprus (NC). This study aimed to determine the presence of pvl among MRSA isolates from patients admitted to a university hospital in NC using molecular methods. Fifty S. aureus strains were included in this study. BD Phoenix automated identification system was used for bacterial identification and antibiotic susceptibility testing. Methicillin resistance was confirmed by disc diffusion assay. Presence of nuc and mecA genes was tested by multiplex PCR. Detection of pvl gene was performed by single-target PCR. Results Out of 50 S. aureus isolates identified as MRSA by BD Phoenix system, 3 were susceptible to cefoxitin with disc diffusion assay and were confirmed as methicillin-sensitive S. aureus (MSSA). All isolates (n = 50, 100%) tested positive for the presence nuc gene and 68% (n = 34/50) were mecA positive. pvl was detected in 27.7% (n = 13/47) of the MRSA isolates. Among PVL-positive MRSA isolates, 69.2% (9/13) were inpatients. PVL-MRSA was more common in isolates from deep tracheal aspirate (30.8%, 4/13) and abscess/wound (23.1%, 3/13). This represents the first study of PVL presence among MRSA in hospital setting in NC.


Author(s):  
Takanori Sohda ◽  
Hiroshi Saito ◽  
Goro Asano ◽  
Katsunari Fukushi ◽  
Katsuya Suzuki ◽  
...  

Recently, the functional aspect as well as morphological aspect of the reserve cells in the cervix uteri drew much attention in view of the carcinogenesis in squamocolumunar junction. In this communication, the authors elucidate the ultrastructural features of the reserve cells in patients of various age groups visiting our university hospital and affiliated hospital.From conventional light microscopic point of view, the reserve cells tend to be pronounced in various pathological conditions, such as the persisting inflammation, proliferative disorders and irritation of hormones. The morphological patterns of the reserve cells from various stage and degree of irritation were observed.


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