Expression levels of Notch1 and Delta-like 4 in peripheral lymphocytes and their relationship with T helper 17 (Th17) cells in renal transplant recipients

2015 ◽  
Vol 33 (1) ◽  
pp. 13-19
Author(s):  
Kai Zheng ◽  
Jianming Tan ◽  
Wenping Zhu ◽  
Weizhen Wu ◽  
Shunliang Yang ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Th17 Cells ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
T Helper ◽  
T Helper 17 ◽  
Expression Levels ◽  
Peripheral Lymphocytes

scholarly journals The imbalance between Tregs, Th17 cells and inflammatory cytokines among renal transplant recipients

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Liang Ma ◽  
Huimao Zhang ◽  
Kebang Hu ◽  
Guoyue Lv ◽  
Yaowen Fu ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Inflammatory Cytokines ◽  
Th17 Cells ◽  
Renal Transplant Recipients ◽  
Transplant Recipients

scholarly journals Enzyme-Linked Immunosorbent Spot Assay Analysis of Peripheral Blood Lymphocyte Reactivity to Donor HLA-DR Peptides: Potential Novel Assay for Prediction of Outcomes for Renal Transplant Recipients

2002 ◽  
Vol 13 (1) ◽  
pp. 252-259
Author(s):  
Nader Najafian ◽  
Alan D. Salama ◽  
Eugenia V. Fedoseyeva ◽  
Gilles Benichou ◽  
Mohamed H. Sayegh
Keyword(s):  
High Risk ◽  
Renal Transplant ◽  
Interferon Γ ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
T Helper ◽  
Indirect Pathway ◽  
Creatinine Concentration ◽  
Hla Dr ◽  
Risk Patients

ABSTRACT. Chronic allograft dysfunction, which is the most common cause of late allograft failure, is in part caused by an ongoing immune response orchestrated by T lymphocytes primed by the indirect pathway of allorecognition. The low frequencies of such T cells have made it difficult to study indirect alloreactivity by using currently available assays. The development of a sensitive, clinically useful method of measuring indirect alloreactivity among human renal transplant recipients was thus attempted. Furthermore, in a pilot immunologic study, the contribution of the indirect pathway was studied in two groups of renal transplant recipients, i.e., patients with no prior acute rejection episodes and stable renal function (“stable” patients) and patients with at least one previous episode of biopsy-proven acute rejection, who were thus at risk for the development of chronic rejection (“high-risk” patients). The frequencies of type 1 T helper (interferon-γ-producing) and type 2 T helper (interleukin-5- and -10-producing) peripheral blood lymphocytes reactive with a panel of synthetic peptides (corresponding to sequences from donor HLA-DR molecules) were determined for renal transplant recipients and normal control subjects by using an enzyme-linked immunosorbent spot assay (ELISPOT). Among recipients of DR-mismatched allografts, a cut-off value of 60 interferon-γ spots/106 cells significantly (P = 0.02) separated stable patients (creatinine concentration, 1.1 ± 0.3 mg/dl) from high-risk patients (creatinine concentration, 2.3 ± 1.7 mg/dl). This is the first demonstration that the enzyme-linked immunosorbent spot assay can be used to monitor indirect alloreactivity to donor HLA-DR peptides among renal transplant recipients. These data provide the rationale for the prospective study of indirect alloreactivity among transplant recipients, to allow predictions of which patients would be at risk for the development of chronic rejection and thus allow appropriate planning of future interventions.

scholarly journals P1687ASSOCIATION OF URINARY MICRORNA-21-5P WITH INTERSTITIAL FIBROSIS AND TUBULAR ATROPHY (IFTA) IN RENAL TRANSPLANT RECIPIENTS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Michal Gniewkiewicz ◽  
Izabela Paszkowska ◽  
Jolanta Gozdowska ◽  
Katarzyna Czerwinska ◽  
Anna Sadowska-Jakubowicz ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Renal Allograft ◽  
Interstitial Fibrosis ◽  
Clinicopathological Parameters ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Tubular Atrophy ◽  
Kidney Allograft ◽  
Expression Levels ◽  
Allograft Function

Abstract Background and Aims One of the most limiting factors of long-term graft survival is chronic renal allograft dysfunction (CAD). The major hallmarks of CAD are interstitial fibrosis and tubular atrophy (IFTA). MicroRNAs (miRNAs) are 19-25 nucleotides, small, noncoding molecules involved in the regulation of gene expression. MiRNAs have a role in various immunological processes, including inflammation and fibrosis. Particularly, microRNA-21-5p (miR-21) is reported to be strongly associated with pathogenesis regarding tubulointerstitium. The aim of this study was to analyse expression levels of urinary miR-21 in the renal transplant recipients and evaluate their application in the assessment of IFTA and kidney allograft function. Method The expression levels of urinary miR-21 were measured in 31 renal transplant recipients with biopsy-evaluated IFTA (IFTA 0+I: n=17; IFTA II+III: n=14) by quantitative PCR. Protocolar biopsies were performed 1 or 2 years after renal transplantation. Urine samples were collected at the time of biopsy procedure. MicroRNA-191-5p was used as reference gene. Correlations between the clinicopathological parameters and the level of expression of miR-21 were assessed. Results Relative expression level of miR-21 was significantly increased in IFTA II+III group compared to IFTA 0+I group. MiR-21 correlated positively with serum concentration of creatinine and negatively with eGFR. ROC analysis showed diagnostic value of miR-21 with an area under curve (AUC) of 0.80, high sensitivity and specificity. Conclusion MiR-21 is associated with IFTA and dysfunction of kidney allograft. It may be considered as potential non-invasive biomarker of renal allograft function.

STIMULATED RESPONSE OF PERIPHERAL LYMPHOCYTES MAY DISTINGUISH CYCLOSPORINE EFFECT IN RENAL TRANSPLANT RECIPIENTS RECEIVING A CYCLOSPORINE+RAPAMYCIN REGIMEN1

2000 ◽  
Vol 69 (3) ◽  
pp. 432-436 ◽  
Author(s):  
Rakesh Sindhi ◽  
Mariano F. LaVia ◽  
Emily Paulling ◽  
John McMichael ◽  
Gilbert Burckart ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Peripheral Lymphocytes

scholarly journals Reactivations of Latent Viral Infections Are Associated with an Increased Thr389 p70S6k Phosphorylation in Peripheral Lymphocytes of Renal Transplant Recipients

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 424
Author(s):  
Maxim Cherneha ◽  
Johannes Korth ◽  
Meike Kaulfuß ◽  
Mirko Trilling ◽  
Marek Widera ◽  
...  
Keyword(s):  
Renal Transplant ◽  
B Lymphocytes ◽  
Mononuclear Cells ◽  
Surrogate Marker ◽  
Mtor Pathway ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Peripheral Blood Mononuclear ◽  
Data Set ◽  
Peripheral Lymphocytes

Reactivations of BK polyoma virus (BKPyV) and human cytomegalovirus (HCMV) frequently cause life- and graft-threatening complications after renal transplantation. Both viruses are dependent on the mTOR pathway for replication. In this study we investigated the association of viral replication with mTOR activity in peripheral lymphocytes of renal transplant recipients. A flow-cytometry based assay for the measurement of Thr389 p70S6k phosphorylation, a surrogate marker of the mTOR pathway was established. Forty-eight adult renal transplant recipients were recruited to measure p70S6k activity in their peripheral blood mononuclear cells. This data set in conjunction with information concerning previous replication of BKPyV and HCMV was examined for correlations. Episodes of BKPyV replication were significantly associated with increased p70S6k phosphorylation in CD4+ T lymphocytes (p = 0.0002) and CD19+ B lymphocytes (p = 0.0073). HCMV infection of patients with a high-risk HCMV constellation of donor and recipient (D+/R−) was associated with increased p70S6k phosphorylation in CD19+ B lymphocytes (p = 0.0325). These associations were found to be independent of the trough levels of the immunosuppressive drugs. Conclusion: P70S6k phosphorylation in peripheral lymphocytes is associated with BKPyV reactivations and to a lesser extent with HCMV infections in renal transplant recipients.

Sign in / Sign up

Export Citation Format

Share Document