Low genetic diversity of Anaplasma marginale in calves in an endemic area for bovine anaplasmosis in the state of São Paulo, Brazil

2016 ◽  
Vol 7 (1) ◽  
pp. 20-25 ◽  
Author(s):  
Jenevaldo Barbosa da Silva ◽  
Marcos Rogério André ◽  
Rosangela Zacarias Machado
Keyword(s):  
Genetic Diversity ◽  
Endemic Area ◽  
Anaplasma Marginale ◽  
Sao Paulo ◽  
The State ◽  
São Paulo ◽  
Low Genetic Diversity ◽  
Bovine Anaplasmosis

scholarly journals Serosurvey of Rickettsia spp. in dogs and humans from an endemic area for Brazilian spotted fever in the State of São Paulo, Brazil

2008 ◽  
Vol 24 (2) ◽  
pp. 247-252 ◽  
Author(s):  
Adriano Pinter ◽  
Maurício C. Horta ◽  
Richard C. Pacheco ◽  
Jonas Moraes-Filho ◽  
Marcelo B. Labruna
Keyword(s):  
Human Serum ◽  
Endemic Area ◽  
Spotted Fever ◽  
Sao Paulo ◽  
The State ◽  
The Other ◽  
São Paulo ◽  
Human Sera ◽  
Antibody Titers ◽  
Brazilian Spotted Fever

The present study provides a rickettsial serosurvey in 25 dogs and 35 humans in an endemic area for Brazilian spotted fever in the State of São Paulo, where the tick Amblyomma aureolatum is the main vector. Testing canine and human sera by indirect immunofluorescence against four Rickettsia antigens (R. rickettsii, R. parkeri, R. felis and R. bellii) showed that 16 (64%) of canine sera and 1 (2.8%) of human sera reacted to at least one of these rickettsial antigens with titers <FONT FACE=Symbol>³</FONT> 64. Seven canine sera and the single reactive human serum showed titers to R. rickettsii at least four times those of any of the other three antigens. The antibody titers in these 7 animals and 1 human were attributed to stimulation by R. rickettsii infection. No positive canine or human serum was attributed to stimulation by R. parkeri, R. felis, or R. bellii. Our serological results showed that dogs are important sentinels for the presence of R. rickettsii in areas where the tick A. aureolatum is the main vector of Brazilian spotted fever.

scholarly journals GENOTYPE CHARACTERIZATION OF Leishmania (Viannia) braziliensis ISOLATED FROM HUMAN AND CANINE BIOPSIES WITH AMERICAN CUTANEOUS LEISHMANIASIS

2015 ◽  
Vol 57 (3) ◽  
pp. 257-262
Author(s):  
Lasaro Teixeira FERREIRA ◽  
Aparecida Helena de Souza GOMES ◽  
Vera Lucia PEREIRA-CHIOCCOLA
Keyword(s):  
Genetic Diversity ◽  
Natural Populations ◽  
Sao Paulo ◽  
The State ◽  
Internal Transcribed Spacers ◽  
São Paulo ◽  
Three Samples ◽  
Two Samples ◽  
American Tegumentary Leishmaniasis

Introduction: American tegumentary leishmaniasis (ATL) can be caused by Leishmania (Viannia) braziliensis complex. The evolution of ATL initially results in lesions and can develop into disseminated or diffuse forms. The genetic diversity of L. (V.) braziliensis in some endemic areas of Brazil has been poorly studied, such as in the state of São Paulo. This study analyzed the genetic diversity of L. (V.) braziliensis isolates collected from patients and dogs with LTA from the state of São Paulo. Methods: Leishmaniasis diagnosis was determined by PCR. The 132 biopsies were collected in different regions of Sao Paulo State, Brazil (36 municipalities). The genetic characterization of L. (V.) braziliensis isolates was tested by RFLP-PCR using DNA extracted from biopsies. The primer set amplified a specific region of Leishmania internal transcribed spacers of the ribosomal DNA locus. Results: Of the 132 samples, 52 (40%) were completely genotyped by RFLP-PCR (44 from human patients and eight from dogs). The results showed nine distinct patterns. The majority of the genotyped samples were from Sorocaba (30), and the others were distributed among 14 other municipalities. The first pattern was more frequent (29 samples), followed by pattern 2 (nine samples) and pattern 3 (three samples). Patterns 4, 6, 7, 8 and 9 were composed of two samples each and pattern 5 of one sample. Conclusion: These results suggest that polymorphic strains of L. (V.) braziliensis circulate in the state of São Paulo. These data agree with studies from other regions of Brazil, showing great variability among the natural populations of endemic foci.

Genetic diversity by AFLP analysis within Jatropha curcas L. populations in the State of São Paulo, Brazil

2015 ◽  
Vol 80 ◽  
pp. 316-320 ◽  
Author(s):  
Franciele Pioto ◽  
Renan Souza Costa ◽  
Suzelei Castro França ◽  
Eduardo Antonio Gavioli ◽  
Bianca Waléria Bertoni ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Jatropha Curcas ◽  
Sao Paulo ◽  
The State ◽  
Aflp Analysis ◽  
São Paulo ◽  
Jatropha Curcas L

Genetic diversity of Anaplasma marginale strains from an outbreak of bovine anaplasmosis in an endemic area

2008 ◽  
Vol 158 (1-2) ◽  
pp. 103-109 ◽  
Author(s):  
Consuelo Alamzán ◽  
Citlaly Medrano ◽  
Martín Ortiz ◽  
José de la Fuente
Keyword(s):  
Genetic Diversity ◽  
Endemic Area ◽  
Anaplasma Marginale ◽  
Bovine Anaplasmosis

New Epidemiological Data on Brazilian Spotted Fever in an Endemic Area of the State of São Paulo, Brazil

2009 ◽  
Vol 9 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Jonas Moraes-Filho ◽  
Adriano Pinter ◽  
Richard C. Pacheco ◽  
Thais B. Gutmann ◽  
Sonia O. Barbosa ◽  
...  
Keyword(s):  
Endemic Area ◽  
Spotted Fever ◽  
Epidemiological Data ◽  
Sao Paulo ◽  
The State ◽  
São Paulo ◽  
Brazilian Spotted Fever

scholarly journals Genetic diversity of environmental Aspergillus flavus strains in the state of São Paulo, Brazil by random amplified polymorphic DNA

2007 ◽  
Vol 102 (6) ◽  
pp. 687-692 ◽  
Author(s):  
Alexandre Lourenço ◽  
Edison Luís Durigon ◽  
Paolo Zanotto ◽  
Jovita Eugênia Gazzinelli Cruz Madeira ◽  
Adriana Palma De Almeida ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Aspergillus Flavus ◽  
Random Amplified Polymorphic Dna ◽  
Sao Paulo ◽  
The State ◽  
São Paulo

Spotted fever in Brazil: a seroepidemiological study and description of clinical cases in an endemic area in the state of São Paulo.

2001 ◽  
Vol 65 (4) ◽  
pp. 329-334 ◽  
Author(s):  
E R de Lemos ◽  
R C Ravagnani ◽  
S R Zaki ◽  
T L Ferebee ◽  
F C Ferreira ◽  
...  
Keyword(s):  
Endemic Area ◽  
Spotted Fever ◽  
Sao Paulo ◽  
The State ◽  
São Paulo ◽  
Clinical Cases

scholarly journals Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

2015 ◽  
Vol 24 (4) ◽  
pp. 438-446 ◽  
Author(s):  
Rosangela Zacarias Machado ◽  
Jenevaldo Barbosa da Silva ◽  
Marcos Rogério André ◽  
Luiz Ricardo Gonçalves ◽  
Carlos Antonio Matos ◽  
...  
Keyword(s):  
Tandem Repeats ◽  
Clinical Signs ◽  
Anaplasma Marginale ◽  
Sao Paulo ◽  
Cattle Herd ◽  
Southeastern Brazil ◽  
São Paulo ◽  
Lactating Cows ◽  
Bovine Anaplasmosis ◽  
Different Strains

Abstract The present study reports the genetic diversity of Anaplasma marginale during anaplasmosis outbreaks in rural properties of the states of Goiás and São Paulo, Brazil. Mortality rates of 3.5% (37/1,050) in calves, 4.7% (45/954) in heifers and 1.1% (25/2,200) in lactating cows were observed in a cattle herd of the municipality of Mambaí, state of Goiás, central-western Brazil. In a cattle herd from the municipality of Lins, state of São Paulo, in southeastern Brazil, none of the animals died, despite presenting clinical signs suggestive of bovine anaplasmosis and exhibiting a drastic decrease in milk production. Thus, blood samples were collected from 100 animals with clinical signs suggestive of bovine anaplasmosis in the municipalities of Mambaí and Lins. Based on the microsatellite structure of the MSP1a of A. marginale, the genotypes E and H were observed in Lins, and the C, D and E genotypes were found in Mambaí. The analysis of the tandem repeat structures of the MSP1a showed nine different strains (τ-10 -15, α-β2, α-β3-13, α-β2 192, τ-β-100, α-β2-Γ, 193-β-100, 191-13-Γ and 191-13-18) in Lins and two (α-β3-Γ and E-F-φ2-F2) in Mambaí. Three new tandem repeats of MSP1a (191, 192 and 193) were described. The τ-10-15 and α-β3-Γ strains were predominantly associated with the occurrence of clinical anaplasmosis and mortality in calves, heifers and lactating cows.

Sign in / Sign up

Export Citation Format

Share Document