Phylogenetic analysis, genetic diversity and geographical distribution of Babesia caballi based on 18S rRNA gene

2021 ◽  
pp. 101776
Author(s):  
Anil Kumar Nehra ◽  
Ansu Kumari ◽  
Aman Dev Moudgil ◽  
Sukhdeep Vohra
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Geographical Distribution ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Babesia Caballi

scholarly journals The diversity, distribution and host-parasite associations of trypanosomes in Western Australian wildlife

Parasitology ◽  
2009 ◽  
Vol 136 (11) ◽  
pp. 1269-1279 ◽  
Author(s):  
S. AVERIS ◽  
R. C. A. THOMPSON ◽  
A. J. LYMBERY ◽  
A. F. WAYNE ◽  
K. D. MORRIS ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Geographical Distribution ◽  
Host Species ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Parasite Diversity ◽  
Host Parasite ◽  
Western Australian ◽  
Geographical Locations

SUMMARYLittle is known regarding the diversity, distribution or host-parasite associations of Trypanosoma spp. in Australian wildlife. Here we report on an investigation based on divergence of the 18S rRNA gene of trypanosomes isolated from a range of hosts and varied geographical locations. A total of 371 individuals representing 19 species of native animals from 14 different locations were screened. In total, 32 individuals from 9 different species tested positive for the parasite. Phylogenetic analysis revealed considerable parasite diversity with no clear geographical distribution and no evidence of host specificity. In general, it appears that Australian Trypanosoma spp. are widespread, with several genotypes appearing in multiple host species and in varied locations including both mainland areas and offshore islands. Some host species were found to be susceptible to multiple genotypes, but no individuals were infected with more than a single isolate.

scholarly journals Phylogenetic Analysis of the Hypervariable Region of the 18S rRNA Gene of Cryptosporidium Oocysts in Feces of Canada Geese (Branta canadensis): Evidence for Five Novel Genotypes

2004 ◽  
Vol 70 (1) ◽  
pp. 452-458 ◽  
Author(s):  
Kristen L. Jellison ◽  
Daniel L. Distel ◽  
Harold F. Hemond ◽  
David B. Schauer
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Hypervariable Region ◽  
Pcr Amplification ◽  
The United States ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Canada Geese ◽  
Pcr Products

ABSTRACT To assess genetic diversity in Cryptosporidium oocysts from Canada geese, 161 fecal samples from Canada geese in the United States were analyzed. Eleven (6.8%) were positive for Cryptosporidium spp. following nested PCR amplification of the hypervariable region of the 18S rRNA gene. Nine PCR products from geese were cloned and sequenced, and all nine diverged from previously reported Cryptosporidium 18S rRNA gene sequences. Five sequences were very similar or identical to each other but genetically distinct from that of Cryptosporidium baileyi; two were most closely related to, but genetically distinct from, the first five; and two were distinct from any other sequence analyzed. One additional sequence in the hypervariable region of the 18S rRNA gene isolated from a cormorant was identical to that of C. baileyi. Phylogenetic analysis provided evidence for new genotypes of Cryptosporidium species in Canada geese. Results of this study suggest that the taxonomy of Cryptosporidium species in geese is complex and that a more complete understanding of genetic diversity among these parasites will facilitate our understanding of oocyst sources and species in the environment.

Phylogenetic analysis based on 18S rRNA gene sequences ofSchellackiaparasites (Apicomplexa: Lankesterellidae) reveals their close relationship to the genusEimeria

Parasitology ◽  
2013 ◽  
Vol 140 (9) ◽  
pp. 1149-1157 ◽  
Author(s):  
R. MEGÍA-PALMA ◽  
J. MARTÍNEZ ◽  
S. MERINO
Keyword(s):  
Phylogenetic Analysis ◽  
Blood Cells ◽  
18S Rrna ◽  
Taxonomic Status ◽  
Refractile Body ◽  
18S Rrna Gene ◽  
Blood Parasites ◽  
Recent Common Ancestor ◽  
Rrna Gene ◽  
Amphibian Species

SUMMARYIn the present study we detectedSchellackiahaemoparasites infecting the blood cells ofLacerta schreiberiandPodarcis hispanica, two species of lacertid lizards from central Spain. The parasite morphometry, the presence of a refractile body, the type of infected blood cells, the kind of host species, and the lack of oocysts in the fecal samples clearly indicated these blood parasites belong to the genusSchellackia. Until now, the species of this genus have never been genetically characterized and its taxonomic position under the Lankesterellidae family is based on the lack of the exogenous oocyst stage. However, the phylogenetic analysis performed on the basis of the 18S rRNA gene sequence revealed that species of the genusSchellackiaare clustered withEimeriaspecies isolated from a snake and an amphibian species but not withLankesterellaspecies. The phylogenetic analysis rejects that both genera share a recent common ancestor. Based on these results we suggest a revision of the taxonomic status of the family Lankesterellidae.

Genetic Diversity of Theileria equi from Horses in Different Regions of Brazil Based on the 18S rRNA Gene

10.1645/18-34 ◽  
2019 ◽  
Vol 105 (1) ◽  
pp. 186 ◽  
Author(s):  
Gabriela L. V. Vitari ◽  
Renata L. Costa ◽  
Ana Paula M. Abreu ◽  
Maristela Peckle ◽  
Claudia B. Silva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Theileria Equi

scholarly journals Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa

2009 ◽  
Vol 159 (2) ◽  
pp. 112-120 ◽  
Author(s):  
Raksha Bhoora ◽  
Linda Franssen ◽  
Marinda C. Oosthuizen ◽  
Alan J. Guthrie ◽  
Erich Zweygarth ◽  
...  
Keyword(s):  
South Africa ◽  
18S Rrna ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Sequence Heterogeneity

Effect of Cell Culture on 18S rRNA Gene Sequences in the Cultural Course of Taxus chinensis Cells

2008 ◽  
Vol 63 (1-2) ◽  
pp. 127-132
Author(s):  
Fu Xiang ◽  
Long J. Yu ◽  
Wu Chen ◽  
Zhi Liu
Keyword(s):  
Cell Culture ◽  
Phylogenetic Analysis ◽  
18S Rrna ◽  
Relative Rate ◽  
18S Rrna Gene ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Taxus Chinensis ◽  
Gene Sequences ◽  
Parsimony Method

Cell culture is an effective technology for taxol production. This paper discusses the effect of Taxus cell cultures on the 18S rRNA gene sequences based on the phylogenetic analysis of cultured T. chinensis cells and related species. The phylogenetic tree is reconstructed using the maximum parsimony method and the relative rate test to test the hypothesis of a molecular clock. The phylogenetic analysis indicates that cell culture changes the phylogenetic position of cultured T. chinensis cells. More than that, the 18S rRNA gene of cultured T. chinensis cells has a faster rate of substitution than that of T. chinensis. With T. media as reference, the divergence time of the cultured T. chinensis cells is 7 Ma (million years) more than that of the T. chinensis cells based on the 18S rRNA gene sequences.

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