Salmonella typhimurium grown in iron-rich media, inactivated with ferric chloride and adjuvanted with homologous bacterial DNA is potent and efficacious vaccine in mice

Vaccine ◽  
2005 ◽  
Vol 23 (48-49) ◽  
pp. 5590-5598 ◽  
Author(s):  
Devender Kumar ◽  
Ajit Singh
Keyword(s):  
Salmonella Typhimurium ◽  
Ferric Chloride ◽  
Bacterial Dna ◽  
Rich Media ◽  
Efficacious Vaccine

scholarly journals Pasteurella multocida inactivated with ferric chloride and adjuvanted with bacterial DNA is a potent and efficacious vaccine in Balb/c mice

2018 ◽  
Vol 67 (9) ◽  
pp. 1383-1390
Author(s):  
Maryam Homayoon ◽  
Yahya Tahamtan ◽  
Mohammad Kargar ◽  
Seyed Mohammad Hossein Hosseini ◽  
Abbas Akhavan Sepahy
Keyword(s):  
Ferric Chloride ◽  
Pasteurella Multocida ◽  
Bacterial Dna ◽  
Efficacious Vaccine

scholarly journals DNA topology and adaptation of salmonella typhimurium to an intracellular environment

2000 ◽  
Vol 355 (1397) ◽  
pp. 565-574 ◽  
Author(s):  
David G. Marshall ◽  
Frances Bowe ◽  
Christine Hale ◽  
Gordon Dougan ◽  
Charles J. Dorman
Keyword(s):  
Salmonella Typhimurium ◽  
Topoisomerase I ◽  
Dna Supercoiling ◽  
Dna Topology ◽  
Integration Host Factor ◽  
Reporter Plasmid ◽  
Bacterial Dna ◽  
Intracellular Environment ◽  
Expression Of Genes ◽  
Gene Coding

The expression of genes coding for determinants of DNA topology in the facultative intracellular pathogen Salmonella typhimurium was studied during adaptation by the bacteria to the intracellular environment of J774A.1 macrophage–like cells. A reporter plasmid was used to monitor changes in DNA supercoiling during intracellular growth. Induction of the dps and spv genes, previously shown to be induced in the macrophage, was detected, as was expression of genes coding for DNA gyrase, integration host factor and the nucleoid–associated protein H–NS. The top A gene, coding for the DNA relaxing enzyme topoisomerase I, was not induced. Reporter plasmid data showed that bacterial DNA became relaxed following uptake of S. typhimurium cells by the macrophage. These data indicate that DNA topology in S. typhimurium undergoes significant changes during adaptation to the intracellular environment. A model describing how this process may operate is discussed.

scholarly journals SPECIALIZED TRANSDUCING PHAGES DERIVED FROM PHAGE P22 THAT CARRY THE proAB REGION OF THE HOST, SALMONELLA TYPHIMURIUM: GENETIC EVIDENCE FOR THEIR STRUCTURE AND MODE OF TRANSDUCTION

Genetics ◽  
1976 ◽  
Vol 83 (3) ◽  
pp. 459-475
Author(s):  
Adrienne P Jessop
Keyword(s):  
Salmonella Typhimurium ◽  
Mixed Infection ◽  
Genetic Evidence ◽  
Lytic Cycle ◽  
Single Infection ◽  
Wild Type ◽  
Bacterial Dna ◽  
Phage P22

ABSTRACT Two independently isolated specialized transducing phages, P22pro-1 and P22pro-3, have been studied. Lysates of P22pro-1contain a majority of transducing phages which can go through the lytic cycle only in mixed infection; these defective phages transduce by lysogenization in mixed infection and by substitution in single infection. A few of the transducing phages in P22pro-1 lysates appear to be non-defective, being able to form plaques and to transduce by lysogenization in single infection. Transduction by P22pro-3 lysates is effected by non-defective transducing phages, which transduce by lysogenization; these lysates also contain a majority of defective phages which do not co-operate in mixed infection. The P22pro-1 genome is thought to contain an insertion of bacterial DNA longer than the terminal repetition present in P22 wild type, so that at maturation a population of differently defective phages is produced. The exact structure of the P22pro-3 genome is open to conjecture, but it seems clear that the insertion of bacterial DNA is smaller than that in P22pro-1. Both P22pro-1 and P22pro-3 are defective in integration at ataA under non-selective conditions, although both integrate on medium that lacks proline.

scholarly journals Capsaicin potently blocks Salmonella typhimurium invasion of Vero cells

2022 ◽  
Author(s):  
Joseph A Ayariga ◽  
Daniel Abugri ◽  
Balagopal Amrutha ◽  
Robert Villafane
Keyword(s):  
Salmonella Typhimurium ◽  
Animal Cell ◽  
Antimicrobial Property ◽  
Bioactive Compound ◽  
The Elderly ◽  
Vero Cells ◽  
The United States ◽  
Potent Inhibition ◽  
Efficacious Vaccine

As at 2021, the center for disease control (CDC) reported that Salmonella causes 1.2 million illness in the United States each year, with a mortality rate approaching 500 deaths per annum. Infants, the elderly, and persons with compromised immunity are the population with higher risk of mortality from this infection. At present there is no commercially available, safe and efficacious vaccine for the control and management of Salmonella typhimurium (S. typhimurium). More so, S. typhimurium has been shown to develop resistance against most antibiotics used for treatment of the infection. Capsaicin, a bioactive compound from Capsicum chinense (C. chinenses) is undoubtedly one of the most widely used spice in the world. This heat producing compound is not only been used as food additive but have been demonstrated to possess unique properties that have pharmacological, physiological, and antimicrobial applications. In this work, the antimicrobial property of pure capsaicin or capsaicin extract against S. typhimurium is tested to determine the compounds effectiveness in S. typhimurium inhibition. Capsaicin extract showed potent inhibition of S. typhimurium growth at concentrations as low as 100 ng/ml, whereas pure capsaicin comparatively showed poorer inhibition of the bacteria. Furthermore, both capsaicin extract and pure capsaicin potently blocked S. typhimurium invasion of an animal cell line in vitro. Taken together, this work revealed that capsaicin might work synergistically with dihydrocapsaicin or the other capsaicinoids to inhibit S. typhimurium growth, whereas individually, capsaicin or dihydrocapsaicin could potently block the bacteria entry and invasion of Vero cells.

115: Detection and Identification of Bacterial DNA and HIV Gag in the Semen of HIV Infected Men

2004 ◽  
Vol 171 (4S) ◽  
pp. 30-30
Author(s):  
Robert C. Eyre ◽  
Ann A. Kiessling ◽  
Thomas E. Mullen ◽  
Rachel L. Kiessling
Keyword(s):  
Bacterial Dna ◽  
Detection And Identification

Bacterial DNA in non-leukocytic ascites, identification of risk factors

2014 ◽  
Vol 52 (08) ◽  
Author(s):  
C Engelmann ◽  
S Krohn ◽  
D Prywerek ◽  
K Zeller ◽  
D Deichsel ◽  
...  
Keyword(s):  
Risk Factors ◽  
Bacterial Dna
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