Vaccine draining lymph nodes are a source of antigen-specific B cells

Vaccine ◽  
2017 ◽  
Vol 35 (9) ◽  
pp. 1259-1265 ◽  
Author(s):  
Stephanie C. Pero ◽  
Yu-Jing Sun ◽  
Girja S. Shukla ◽  
Chelsea L. Carman ◽  
Christopher C. Krag ◽  
...  
Keyword(s):  
B Cells ◽  
Lymph Nodes ◽  
Draining Lymph Nodes

Granzyme B production by activated B cells derived from breast cancer-draining lymph nodes

2019 ◽  
Vol 114 ◽  
pp. 172-178 ◽  
Author(s):  
Mohsen Arabpour ◽  
Reza Rasolmali ◽  
Abdoul-Rasoul Talei ◽  
Fereshteh Mehdipour ◽  
Abbas Ghaderi
Keyword(s):  
Breast Cancer ◽  
B Cells ◽  
Lymph Nodes ◽  
Granzyme B ◽  
Draining Lymph Nodes ◽  
Activated B Cells

OR.39. Enumeration and Phenotype of Autoreactive B Cells in Pancreatic Draining Lymph Nodes from Type 1 Diabetes Subjects

2009 ◽  
Vol 131 ◽  
pp. S19
Author(s):  
Sally Kent ◽  
Elizabeth Bradshaw ◽  
Qing Han ◽  
Navin Varadarajan ◽  
Allison Greer ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
B Cells ◽  
Lymph Nodes ◽  
Autoreactive B Cells ◽  
Draining Lymph Nodes

scholarly journals An R848 adjuvanted influenza vaccine promotes early activation of B cells in the draining lymph nodes of non-human primate neonates

Immunology ◽  
2017 ◽  
Vol 153 (3) ◽  
pp. 357-367 ◽  
Author(s):  
Beth C. Holbrook ◽  
S. Tyler Aycock ◽  
Emily Machiele ◽  
Elene Clemens ◽  
Danielle Gries ◽  
...  
Keyword(s):  
B Cells ◽  
Influenza Vaccine ◽  
Lymph Nodes ◽  
Early Activation ◽  
Draining Lymph Nodes ◽  
Human Primate

scholarly journals Expression of MIP-1α (CCL3) by a Recombinant Rabies Virus Enhances Its Immunogenicity by Inducing Innate Immunity and Recruiting Dendritic Cells and B Cells

2010 ◽  
Vol 84 (18) ◽  
pp. 9642-9648 ◽  
Author(s):  
Ling Zhao ◽  
Harufusa Toriumi ◽  
Hualei Wang ◽  
Yi Kuang ◽  
Xiaofeng Guo ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Dendritic Cells ◽  
B Cells ◽  
Lymph Nodes ◽  
Rabies Virus ◽  
Mouse Brain ◽  
Peripheral Blood ◽  
Neutralizing Antibody ◽  
Antibody Titers ◽  
Draining Lymph Nodes

ABSTRACT Previously, we showed that overexpression of MIP-1α in mouse brain further decreased rabies virus (RABV) pathogenicity (L. Zhao, H. Toriumi, Y. Kuang, H. Chen, and Z. F. Fu, J. Virol., 83:11808-11818, 2009). In the present study, the immunogenicity of recombinant RABV expressing MIP-1α (rHEP-MIP1α) was determined. It was found that intramuscular immunization of BALB/c mice with rHEP-MIP1α resulted in a higher level of expression of MIP-1α at the site of inoculation, increased recruitment of dendritic cells (DCs) and mature B cells into the draining lymph nodes and the peripheral blood, and higher virus-neutralizing antibody titers than immunization with the parent rHEP and recombinant RABVs expressing RANTES (CCL5) or IP-10 (CXCL10). Our data thus demonstrate that expression of MIP-1α not only reduces viral pathogenicity but also enhances immunogenicity by recruiting DCs and B cells to the site of immunization, the lymph nodes, and the blood.

scholarly journals Regulatory B cells preferentially accumulate in tumor-draining lymph nodes and promote tumor growth

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Sheila N. Ganti ◽  
Tina C. Albershardt ◽  
Brian M. Iritani ◽  
Alanna Ruddell
Keyword(s):  
B Cells ◽  
Tumor Growth ◽  
Lymph Nodes ◽  
Regulatory B Cells ◽  
Promote Tumor Growth ◽  
Draining Lymph Nodes

Exogenous Small Ruminant Betaretrovirus Envelope Protein Is Detected in Draining Lymph Nodes in Contagious Respiratory Tumors of Sheep and Goats

2020 ◽  
pp. 030098582098071
Author(s):  
Marcelo De las Heras ◽  
Raúl Armando Reséndiz ◽  
José María González-Sáinz ◽  
Aurora Ortín
Keyword(s):  
B Cells ◽  
T Lymphocytes ◽  
Lymph Nodes ◽  
Envelope Protein ◽  
Regional Lymph Nodes ◽  
Sheep And Goats ◽  
Nasal Tumor ◽  
Nasal Adenocarcinoma ◽  
Draining Lymph Nodes ◽  
Control Samples

Contagious respiratory tumors of sheep and goats are epithelial neoplasms of the lung and nasal cavities. They are associated with oncogenic betaretroviruses known as jaagsiekte sheep retrovirus and enzootic nasal tumor retrovirus of sheep and goats. We investigated the presence of the envelope protein (ENV) of these retroviruses in retropharyngeal and mediastinal lymph nodes using a specific monoclonal antibody by immunohistochemistry methods, single-labeled or combined with ovine B or T lymphocytes or macrophage cell markers. Samples of lymph nodes, fixed in formalin and zinc fixative, were obtained from paraffin-embedded material. Four groups of samples were used: 24 natural cases of ovine pulmonary adenocarcinoma (OPA), 13 of enzootic nasal adenocarcinoma of sheep (ENAS), 19 of enzootic nasal adenocarcinoma of goats (ENAG), and 14 control samples. ENV was detected by single labeling in cortical lymphoid follicles. Six of 24 OPA samples were positive and only in those from sheep with extensive neoplasia. Immunolabeling was detected in 5/13 ENAS and 10/19 ENAG samples. Positive labeling was found either in the intercellular spaces, membranes, or cytoplasm of cells in follicles. Control samples were not correspondingly labeled. Double immunohistochemistry demonstrated co-labeling of ENV and CD21 (B cells and follicular dendritic cells) in all samples, CD14 (macrophage) in OPA samples, and Pax-5 (B cells) in ENAG samples, but not with CD8 or CD4 (T lymphocytes). These results demonstrate the presence of betaretrovirus ENV proteins in nontumor cells in regional lymph nodes in sheep and goats with contagious respiratory tumors.

Mesenchymal stem cells induced anti-inflammatory features in B cells from breast tumor draining lymph nodes

2018 ◽  
Vol 42 (12) ◽  
pp. 1658-1669 ◽  
Author(s):  
Fereshteh Mehdipour ◽  
Mahboobeh Razmkhah ◽  
Somayeh Rezaeifard ◽  
Mandana Bagheri ◽  
Abdol-Rasoul Talei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
B Cells ◽  
Lymph Nodes ◽  
Breast Tumor ◽  
Anti Inflammatory ◽  
Draining Lymph Nodes

scholarly journals B cells in tumor draining lymph nodes act as efficient antigen presenting cells in cancer patients

2015 ◽  
Vol 3 (S2) ◽  
Author(s):  
A Ali Zirakzadeh ◽  
David Krantz ◽  
Malin Winerdal ◽  
Christian Lundgren ◽  
Ciputra Adijaya Hartana ◽  
...  
Keyword(s):  
B Cells ◽  
Lymph Nodes ◽  
Cancer Patients ◽  
Antigen Presenting Cells ◽  
Antigen Presenting ◽  
Draining Lymph Nodes

scholarly journals Activated, Pro-Inflammatory Th1, Th17, and Memory CD4+ T Cells and B Cells Are Involved in Delayed-Type Hypersensitivity Arthritis (DTHA) Inflammation and Paw Swelling in Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Gaoyang Li ◽  
Shrikant Shantilal Kolan ◽  
Shuai Guo ◽  
Katarzyna Marciniak ◽  
Pratibha Kolan ◽  
...  
Keyword(s):  
T Cells ◽  
B Cells ◽  
Lymph Nodes ◽  
Cd4 T Cells ◽  
Memory T Cells ◽  
T Cell Subsets ◽  
Delayed Type Hypersensitivity ◽  
Memory Cd4 T Cells ◽  
Draining Lymph Nodes

Delayed-type hypersensitivity arthritis (DTHA) is a recently established experimental model of rheumatoid arthritis (RA) in mice with pharmacological values. Despite an indispensable role of CD4+ T cells in inducing DTHA, a potential role for CD4+ T cell subsets is lacking. Here we have quantified CD4+ subsets during DTHA development and found that levels of activated, pro-inflammatory Th1, Th17, and memory CD4+ T cells in draining lymph nodes were increased with differential dynamic patterns after DTHA induction. Moreover, according to B-cell depletion experiments, it has been suggested that this cell type is not involved in DTHA. We show that DTHA is associated with increased levels of B cells in draining lymph nodes accompanied by increased levels of circulating IgG. Finally, using the anti-rheumatoid agents, methotrexate (MTX) and the anti-inflammatory drug dexamethasone (DEX), we show that MTX and DEX differentially suppressed DTHA-induced paw swelling and inflammation. The effects of MTX and DEX coincided with differential regulation of levels of Th1, Th17, and memory T cells as well as B cells. Our results implicate Th1, Th17, and memory T cells, together with activated B cells, to be involved and required for DTHA-induced paw swelling and inflammation.

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