An R848 adjuvanted influenza vaccine promotes early activation of B cells in the draining lymph nodes of non-human primate neonates

ABSTRACT Previously, we showed that overexpression of MIP-1α in mouse brain further decreased rabies virus (RABV) pathogenicity (L. Zhao, H. Toriumi, Y. Kuang, H. Chen, and Z. F. Fu, J. Virol., 83:11808-11818, 2009). In the present study, the immunogenicity of recombinant RABV expressing MIP-1α (rHEP-MIP1α) was determined. It was found that intramuscular immunization of BALB/c mice with rHEP-MIP1α resulted in a higher level of expression of MIP-1α at the site of inoculation, increased recruitment of dendritic cells (DCs) and mature B cells into the draining lymph nodes and the peripheral blood, and higher virus-neutralizing antibody titers than immunization with the parent rHEP and recombinant RABVs expressing RANTES (CCL5) or IP-10 (CXCL10). Our data thus demonstrate that expression of MIP-1α not only reduces viral pathogenicity but also enhances immunogenicity by recruiting DCs and B cells to the site of immunization, the lymph nodes, and the blood.

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Regulatory B cells preferentially accumulate in tumor-draining lymph nodes and promote tumor growth

Scientific Reports ◽

10.1038/srep12255 ◽

2015 ◽

Vol 5 (1) ◽

Cited By ~ 30

Author(s):

Sheila N. Ganti ◽

Tina C. Albershardt ◽

Brian M. Iritani ◽

Alanna Ruddell

Keyword(s):

B Cells ◽

Tumor Growth ◽

Lymph Nodes ◽

Regulatory B Cells ◽

Promote Tumor Growth ◽

Draining Lymph Nodes

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Exogenous Small Ruminant Betaretrovirus Envelope Protein Is Detected in Draining Lymph Nodes in Contagious Respiratory Tumors of Sheep and Goats

Veterinary Pathology ◽

10.1177/0300985820980711 ◽

2020 ◽

pp. 030098582098071

Author(s):

Marcelo De las Heras ◽

Raúl Armando Reséndiz ◽

José María González-Sáinz ◽

Aurora Ortín

Keyword(s):

B Cells ◽

T Lymphocytes ◽

Lymph Nodes ◽

Envelope Protein ◽

Regional Lymph Nodes ◽

Sheep And Goats ◽

Nasal Tumor ◽

Nasal Adenocarcinoma ◽

Draining Lymph Nodes ◽

Control Samples

Contagious respiratory tumors of sheep and goats are epithelial neoplasms of the lung and nasal cavities. They are associated with oncogenic betaretroviruses known as jaagsiekte sheep retrovirus and enzootic nasal tumor retrovirus of sheep and goats. We investigated the presence of the envelope protein (ENV) of these retroviruses in retropharyngeal and mediastinal lymph nodes using a specific monoclonal antibody by immunohistochemistry methods, single-labeled or combined with ovine B or T lymphocytes or macrophage cell markers. Samples of lymph nodes, fixed in formalin and zinc fixative, were obtained from paraffin-embedded material. Four groups of samples were used: 24 natural cases of ovine pulmonary adenocarcinoma (OPA), 13 of enzootic nasal adenocarcinoma of sheep (ENAS), 19 of enzootic nasal adenocarcinoma of goats (ENAG), and 14 control samples. ENV was detected by single labeling in cortical lymphoid follicles. Six of 24 OPA samples were positive and only in those from sheep with extensive neoplasia. Immunolabeling was detected in 5/13 ENAS and 10/19 ENAG samples. Positive labeling was found either in the intercellular spaces, membranes, or cytoplasm of cells in follicles. Control samples were not correspondingly labeled. Double immunohistochemistry demonstrated co-labeling of ENV and CD21 (B cells and follicular dendritic cells) in all samples, CD14 (macrophage) in OPA samples, and Pax-5 (B cells) in ENAG samples, but not with CD8 or CD4 (T lymphocytes). These results demonstrate the presence of betaretrovirus ENV proteins in nontumor cells in regional lymph nodes in sheep and goats with contagious respiratory tumors.

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Mesenchymal stem cells induced anti-inflammatory features in B cells from breast tumor draining lymph nodes

Cell Biology International ◽

10.1002/cbin.11062 ◽

2018 ◽

Vol 42 (12) ◽

pp. 1658-1669 ◽

Cited By ~ 2

Author(s):

Fereshteh Mehdipour ◽

Mahboobeh Razmkhah ◽

Somayeh Rezaeifard ◽

Mandana Bagheri ◽

Abdol-Rasoul Talei ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

B Cells ◽

Lymph Nodes ◽

Breast Tumor ◽

Anti Inflammatory ◽

Draining Lymph Nodes

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Vaccine draining lymph nodes are a source of antigen-specific B cells

Vaccine ◽

10.1016/j.vaccine.2017.01.036 ◽

2017 ◽

Vol 35 (9) ◽

pp. 1259-1265 ◽

Cited By ~ 3

Author(s):

Stephanie C. Pero ◽

Yu-Jing Sun ◽

Girja S. Shukla ◽

Chelsea L. Carman ◽

Christopher C. Krag ◽

...

Keyword(s):

B Cells ◽

Lymph Nodes ◽

Draining Lymph Nodes

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B cells in tumor draining lymph nodes act as efficient antigen presenting cells in cancer patients

Journal for ImmunoTherapy of Cancer ◽

10.1186/2051-1426-3-s2-p65 ◽

2015 ◽

Vol 3 (S2) ◽

Author(s):

A Ali Zirakzadeh ◽

David Krantz ◽

Malin Winerdal ◽

Christian Lundgren ◽

Ciputra Adijaya Hartana ◽

...

Keyword(s):

B Cells ◽

Lymph Nodes ◽

Cancer Patients ◽

Antigen Presenting Cells ◽

Antigen Presenting ◽

Draining Lymph Nodes

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Differential expression of CD86, CD40, and IaK on B cells isolated from draining lymph nodes (DLN) of allergen or irritant treated mice

Journal of Dermatological Science ◽

10.1016/s0923-1811(98)84029-2 ◽

1998 ◽

Vol 16 ◽

pp. S172

Author(s):

GF Gerberick ◽

LW Cruse ◽

CM Miller ◽

GM Ridder

Keyword(s):

B Cells ◽

Lymph Nodes ◽

Differential Expression ◽

Draining Lymph Nodes

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The Early Activation ofCD8+T Cells Is Dependent on Type I IFN Signaling following Intramuscular Vaccination of Adenovirus Vector

BioMed Research International ◽

10.1155/2014/158128 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 5

Author(s):

Masahisa Hemmi ◽

Masashi Tachibana ◽

Sayaka Tsuzuki ◽

Masaki Shoji ◽

Fuminori Sakurai ◽

...

Keyword(s):

Immune Response ◽

Lymph Nodes ◽

Cytotoxic T Lymphocytes ◽

Adenovirus Vector ◽

Innate Immune ◽

Type I ◽

Type I Ifn ◽

Early Activation ◽

Innate Immune Signaling ◽

Draining Lymph Nodes

Few of the vaccines in current use can induce antigen- (Ag-) specific immunity in both mucosal and systemic compartments. Hence, the development of vaccines that realize both mucosal and systemic protection against various pathogens is a high priority in global health. Recently, it has been reported that intramuscular (i.m.) vaccination of an adenovirus vector (Adv) can induce Ag-specific cytotoxic T lymphocytes (CTLs) in both systemic and gut mucosal compartments. We previously revealed that type I IFN signaling is required for the induction of gut mucosal CTLs, not systemic CTLs. However, the molecular mechanism via type I IFN signaling is largely unknown. Here, we report that type I IFN signaling following i.m. Adv vaccination is required for the expression of type I IFN in the inguinal lymph nodes (iLNs), which are the draining lymph nodes of the administration site. We also showed that the type I IFN signaling is indispensable for the early activation of CTLs in iLNs. These data suggested that type I IFN signaling has an important role in the translation of systemic innate immune response into mucosal adaptive immunity by amplifying the innate immune signaling and activating CTLs in the iLN.

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