Purification and determination of C-reactive protein and inter-α-trypsin inhibitor heavy chain 4 in dogs after major surgery through generation of specific antibodies

2016 ◽  
Vol 179 ◽  
pp. 26-31 ◽  
Author(s):  
L. Soler ◽  
N. García ◽  
A. Unzueta ◽  
M. Piñeiro ◽  
M.A. Álava ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Trypsin Inhibitor ◽  
Major Surgery ◽  
C Reactive Protein ◽  
Specific Antibodies ◽  
Reactive Protein

C-REACTIVE PROTEIN IN THE SERUM IN NEPHROSIS AND ACUTE GLOMERULONEPHRITIS

PEDIATRICS ◽  
1960 ◽  
Vol 25 (1) ◽  
pp. 106-111
Author(s):  
Aree Valyasevi ◽  
Joseph M. Sloan ◽  
Lewis A. Barness
Keyword(s):  
Nephrotic Syndrome ◽  
Hormone Therapy ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Positive Test ◽  
Acute Glomerulonephritis ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation

C-reactive protein in the serum and the erythrocyte sedimentation rate were followed serially in 13 patients with acute nephritis and in 9 patients with the nephrotic syndrome. Although a positive C-reactive protein test was always associated with evidence of infection, obvious infection in a few instances was not accompanied by a positive test. Determination of C-reactive protein is valuable in determining the presence of infection in patients with acute nephritis and the nephrotic syndrome, especially in the latter where hormone therapy may mask many signs of infection.

Comparison of Three Commercial Assays for the Determination of Serum C-Reactive Protein

1999 ◽  
Vol 7 (2) ◽  
pp. 45-59
Author(s):  
TARIF ZAWAWI ◽  
IBRAHTM HASHIM ◽  
WALEED AL-YAFI ◽  
MOHAMMED ABDELLAAL
Keyword(s):  
C Reactive Protein ◽  
Reactive Protein

scholarly journals Serum IgM and C-Reactive Protein Binding to Phosphorylcholine of NontypeableHaemophilus influenzaeIncreases Complement-Mediated Killing

2019 ◽  
Vol 87 (8) ◽  
Author(s):  
Jeroen D. Langereis ◽  
Eva S. van der Pasch ◽  
Marien I. de Jonge
Keyword(s):  
Respiratory Tract ◽  
Lower Respiratory Tract ◽  
Classical Pathway ◽  
Upper Respiratory Tract ◽  
C Reactive Protein ◽  
Surface Binding ◽  
Specific Antibodies ◽  
Bacterial Surface ◽  
Reactive Protein ◽  
Tract Infections

ABSTRACTNontypeableHaemophilus influenzae(NTHi) colonizes the human upper respiratory tract without causing disease symptoms, but it is also a major cause of upper and lower respiratory tract infections in children and elderly, respectively. NTHi synthesizes various molecules to decorate its lipooligosaccharide (LOS), which modulates the level of virulence. The presence of phosphorylcholine (PCho) on NTHi LOS increases adhesion to epithelial cells, which is an advantage for the bacterium enabling nasopharyngeal colonization. However, when PCho is incorporated on the LOS of NTHi, it is recognized by the acute-phase C-reactive protein (CRP) and PCho-specific antibodies, both potent initiators of the classical pathway of complement activation. We determined the presence of PCho and binding of IgG and IgM to the bacterial surface for 319 NTHi strains collected from the nasopharynx/oropharynx, middle ear, and lower respiratory tract. PCho detection was higher for NTHi strains collected from the nasopharynx/oropharynx, which was associated with increased binding of IgM and IgG to the bacterial surface. Binding of CRP and IgM to the bacterial surface of PChohighNTHi strains increased complement-mediated killing, which was largely dependent on PCho-specific IgM. The levels of PCho-specific IgM varied in sera from 12 healthy individuals, and higher PCho-specific IgM levels were associated with increased complement-mediated killing of a PChohighNTHi strain. In conclusion, incorporation of PCho on the LOS of NTHi marks the bacterium for binding of CRP and IgM, resulting in complement-mediated killing. Therefore, having a lower PCho might be beneficial in situations where sufficient PCho-specific antibodies and complement are present.

scholarly journals Stimulation of Erythrocyte Cell Membrane Scrambling by C-Reactive Protein

2017 ◽  
Vol 41 (2) ◽  
pp. 806-818 ◽  
Author(s):  
Majed Abed ◽  
Christian Thiel ◽  
Syeda T. Towhid ◽  
Kousi Alzoubi ◽  
Sabina Honisch ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Healthy Volunteers ◽  
Annexin V ◽  
Caspase Activation ◽  
C Reactive Protein ◽  
Forward Scatter ◽  
Reactive Protein ◽  
Phosphatidylserine Translocation ◽  
Binding Cell

Background: Eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine-translocation, is triggered by fever and inflammation. Signaling includes increased cytosolic Ca2+-activity ([Ca2+]i), caspase activation, and ceramide. Inflammation is associated with increased plasma concentration of C-reactive protein (CRP). The present study explored whether CRP triggers eryptosis. Methods: Phosphatidylserine abundance at the cell surface was estimated from annexin-V-binding, cell volume from forward scatter, [Ca2+]i from Fluo3-fluorescence, ceramide abundance and caspase-3-activity utilizing FITC-conjugated antibodies. Moreover, blood was drawn from patients with acute appendicitis (9♀,11♂) and healthy volunteers (10♀,10♂) for determination of CRP, blood count and phosphatidylserine. Results: A 48h CRP treatment significantly increased the percentage of annexin-V-binding cells (≥5µg/ml), [Ca2+]i (≥5µg/ml), ceramide (20µg/ml) and caspase-activity (20µg/ml). Annexin-V-binding was significantly blunted by caspase inhibitor zVAD (10µM). The percentage of phosphatidylserine-exposing erythrocytes in freshly drawn blood was significantly higher in appendicitis patients (1.83±0.21%) than healthy volunteers (0.81±0.09%), and significantly higher following a 24h incubation of erythrocytes from healthy volunteers to patient plasma than to plasma from healthy volunteers. The percentage of phosphatidylserine-exposing erythrocytes correlated with CRP plasma concentration. Conclusion: C-reactive protein triggers eryptosis, an effect at least partially due to increase of [Ca2+]i, increase of ceramide abundance and caspase activation.

Concentrations of tumor-associated trypsin inhibitor and C-reactive protein in serum in acute pelvic inflammatory disease.

1989 ◽  
Vol 35 (5) ◽  
pp. 869-871 ◽  
Author(s):  
J Paavonen ◽  
M Lehtinen ◽  
M Lehto ◽  
S Laine ◽  
R Aine ◽  
...  
Keyword(s):  
Pelvic Inflammatory Disease ◽  
Acute Phase ◽  
Trypsin Inhibitor ◽  
Inflammatory Disease ◽  
Acute Phase Proteins ◽  
Endometrial Biopsy ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Acute Pelvic Inflammatory Disease ◽  
Severe Infections

Abstract We measured tumor-associated trypsin inhibitor (TATI) and C-reactive protein (CRP) in serum of 29 patients with proven pelvic inflammatory disease (PID). TATI values were increased in seven (24%), paralleling increases in CRP. TATI was increased by about 3.5-fold in seven of eight patients with CRP concentrations greater than 90 mg/L, but in none of 21 patients with CRP concentrations less than 90 mg/L. TATI concentration and severity of PID as determined by laparoscopy or endometrial biopsy were not correlated. These results suggest that, in severe infections, regulation of TATI synthesis resembles that of acute-phase proteins.

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