Use of a time-resolved immunofluorometric assay for determination of canine C-reactive protein concentrations in whole blood

AbstractThe purpose of the present study was to evaluate de influence of protein–sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive protein is a plasmatic protein used as a biomarker for the diagnosis of a series of health problems such as ulcerative colitis, cardiovascular diseases, metabolic syndrome, due to its essential role in the evolution of chronic inflammation. The sugar–protein interaction was investigated using steady state and time resolved fluorescence. The results revealed that there are more than two classes of tryptophan, with different degree of accessibility for the quencher molecule. Our study also revealed that sugar–protein complexes have superior thermostability, especially after gamma irradiation at 2 kGy, the protein being stable and functional even after 22 days exposure to 40 °C.

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Development of a point-of-care assay system for high-sensitivity C-reactive protein in whole blood

Clinica Chimica Acta ◽

10.1016/s0009-8981(03)00113-x ◽

2003 ◽

Vol 332 (1-2) ◽

pp. 51-59 ◽

Cited By ~ 67

Author(s):

Jae Soon Ahn ◽

Sunga Choi ◽

Sang Ho Jang ◽

Hyuk Jae Chang ◽

Jae Hoon Kim ◽

...

Keyword(s):

Whole Blood ◽

Point Of Care ◽

High Sensitivity ◽

Assay System ◽

C Reactive Protein ◽

Reactive Protein ◽

Point Of Care Assay

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A single determination of C-reactive protein does not suffice to declare a patient with a diagnosis of axial spondyloarthritis ‘CRP-negative’

Arthritis Research & Therapy ◽

10.1186/s13075-018-1707-8 ◽

2018 ◽

Vol 20 (1) ◽

Cited By ~ 8

Author(s):

Robert Landewé ◽

Tommi Nurminen ◽

Owen Davies ◽

Dominique Baeten

Keyword(s):

Axial Spondyloarthritis ◽

C Reactive Protein ◽

Reactive Protein ◽

Single Determination

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Determination of C-Reactive Protein by Electroimmunodiffusion in Blood and CSF of Neurological Patients

European Neurology ◽

10.1159/000114227 ◽

1973 ◽

Vol 9 (4) ◽

pp. 216-223 ◽

Cited By ~ 9

Author(s):

E. Schuller ◽

B. Allinquant

Keyword(s):

C Reactive Protein ◽

Reactive Protein ◽

Neurological Patients

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C-REACTIVE PROTEIN IN THE SERUM IN NEPHROSIS AND ACUTE GLOMERULONEPHRITIS

PEDIATRICS ◽

10.1542/peds.25.1.106 ◽

1960 ◽

Vol 25 (1) ◽

pp. 106-111

Author(s):

Aree Valyasevi ◽

Joseph M. Sloan ◽

Lewis A. Barness

Keyword(s):

Nephrotic Syndrome ◽

Hormone Therapy ◽

Erythrocyte Sedimentation Rate ◽

Sedimentation Rate ◽

Positive Test ◽

Acute Glomerulonephritis ◽

C Reactive Protein ◽

Reactive Protein ◽

Erythrocyte Sedimentation

C-reactive protein in the serum and the erythrocyte sedimentation rate were followed serially in 13 patients with acute nephritis and in 9 patients with the nephrotic syndrome. Although a positive C-reactive protein test was always associated with evidence of infection, obvious infection in a few instances was not accompanied by a positive test. Determination of C-reactive protein is valuable in determining the presence of infection in patients with acute nephritis and the nephrotic syndrome, especially in the latter where hormone therapy may mask many signs of infection.

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