A novel multiplex PCR coupled with Luminex assay for the simultaneous detection of Cryptosporidium spp., Cryptosporidium parvum and Giardia duodenalis

The protozoan Cryptosporidium parvum is one of the major causative pathogens of diarrhoea in young ruminants; therefore, it causes economic losses and impairs animal welfare. Besides C. parvum, there are many other non-infectious and infectious factors, such as rotavirus, Escherichia coli, and Giardia duodenalis, which may lead to diarrhoeic disease in young livestock. Often, more than one infectious agent is detected in affected animals. Little is known about the interactions bet-ween simultaneously occurring pathogens and their potential effects on the course of disease. In this review, a brief overview about pathogens associated with diarrhoea in young ruminants is presented. Furthermore, information about coinfections involving Cryptosporidium is provided.

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A novel nested multiplex PCR for the simultaneous detection and differentiation of Cryptosporidium spp., Enterocytozoon bieneusi and Encephalitozoon intestinalis

Parasitology International ◽

10.1016/j.parint.2014.05.002 ◽

2014 ◽

Vol 63 (5) ◽

pp. 664-669 ◽

Cited By ~ 7

Author(s):

J.M. Rubio ◽

M. Lanza ◽

I. Fuentes ◽

R.H. Soliman

Keyword(s):

Multiplex Pcr ◽

Simultaneous Detection ◽

Enterocytozoon Bieneusi ◽

Cryptosporidium Spp ◽

Encephalitozoon Intestinalis

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VALIDATION OF MULTIPLEX PCR STRATEGY FOR SIMULTANEOUS DETECTION AND IDENTIFICATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS

Indian Journal of Medical Microbiology ◽

10.1016/s0255-0857(21)01815-6 ◽

2008 ◽

Vol 26 (4) ◽

pp. 361-364

Author(s):

S Rallapalli ◽

S Verghese ◽

RS Verma

Keyword(s):

Staphylococcus Aureus ◽

Multiplex Pcr ◽

Methicillin Resistant Staphylococcus Aureus ◽

Simultaneous Detection ◽

Methicillin Resistant ◽

Detection And Identification

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A novel common primer multiplex PCR (CP-M-PCR) method for the simultaneous detection of meat species

Food Control ◽

10.1016/j.foodcont.2008.05.021 ◽

2009 ◽

Vol 20 (4) ◽

pp. 366-370 ◽

Cited By ~ 38

Author(s):

Weibin Bai ◽

Wentao Xu ◽

Kunlun Huang ◽

Yanfang Yuan ◽

Sishuo Cao ◽

...

Keyword(s):

Multiplex Pcr ◽

Simultaneous Detection ◽

Meat Species ◽

Pcr Method

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Use of a Multiplex PCR System for the Simultaneous Detection of Heat Labile Toxin I and Heat Stable Toxin II Genes of Enterotoxigenic Escherichia coli in Skim Milk and Porcine Stool

Journal of Food Protection ◽

10.4315/0362-028x-61.2.141 ◽

1998 ◽

Vol 61 (2) ◽

pp. 141-145 ◽

Cited By ~ 12

Author(s):

HAU-YANG TSEN ◽

LIANG-ZHAO JIAN ◽

WAN-RONG CHI

Keyword(s):

Escherichia Coli ◽

Multiplex Pcr ◽

Simultaneous Detection ◽

Skim Milk ◽

Pcr Primers ◽

Enterotoxigenic Escherichia Coli ◽

Farm Animals ◽

Target Cells ◽

Heat Stable ◽

Heat Labile

Enterotoxigenic Escherichia coli (ETEC) strains which produce heat labile and/or heat stable toxins (LT and ST) may cause diarrhea in humans and farm animals. Using PCR primers specific for the LT I and ST II genes, a multiplex PCR system which allows detection of LT I- and ST II-producing ETEC strains was developed. When skim milk was used for a PCR assay, it was found that if target cells in the sample were precultured in MacConkey broth for 8 h prior to PCR as few as 100 cells per ml of the sample could be detected. Without the preculture step, 104 CFU of target cells per 0.2 g of porcine stool specimen were required to generate visible PCR products. The multiplex PCR System can be used for rapid testing of fecal specimens, food and possibly environmental samples for the presence of ETEC strains.

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