Wound healing potential of chloroform extract of leaf and fruit of Morinda pubescens – An in silico and in vitro approach

Genus Morinda grows in the wild areas and extensively distributed over southern India. In India, many species have been found available, of which Morinda pubescens (Synonym: Morinda tinctoria) predominantly grows as a weed tree in vacant agricultural land. It has been cultivated in different parts of Tamilnadu state of India. It is widely used in the Indian traditional system of medicine. Based on the folklore claim Insilico and Invitro studies were performed using several methods. Docking experiment was carried on with iGemdock, docking software to initially study the energy binding values of constituents. Having studied the various experiments, Glycogen synthase kinase and several MMP’s [1] was used as a protein. Energy binding values showed up prompt results which indicate the affinity of the molecules towards the protein. Plant extract at three different concentrations was taken and MTT assay was performed. Then, with the help of the C2C12 cell line which is a muscle cell line, we evaluated the effect of our plant extract on the cells. Among the chloroform extract of leaf (100µg/ml) and fruit (100µg/ml), fruit extract was found to have the efficiency to fill the gaps between cells with newer ones and better elongation of cells compared to control (Nutrient treated)

PHYTOCHEMICAL EVALUATION AND HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY PROFILING: SAPINDUS EMARGINATUS VAHL. AND MORINDA PUBESCENS J.E.SM. BARKS EXTRACTS

ABSTRACTObjective: To identify the flavonoids high-performance thin layer chromatography (HPTLC) profiles from barks of Sapindus emarginatus Vahl.(Sapindaceae) and Morinda pubescens J.E.Sm. (Rubiaceae) by ethanol extracts.Methods: The barks of S. emarginatus Vahl. and M. pubescens J.E.Sm. extracted each separately with ethanol. Both these ethanol extracts weresubjected to preliminary phytochemical analysis. Based on phytochemical studies, the extracts obtained were subjected to HPTLC profiles for identifyand confirmation flavonoids, both these samples were compared with standard (Rutin). HPTLC analysis performed with silica gel G 60 F254 plateswith mobile phase ethyl acetate:n-butanol:formic acid:water (5:3:1:1). Detection of flavonoid compound was performed by scanning the developedplate at 254 nm.Results: Result of these both extracts shows positive tests for flavonoids. Ethanol extracts barks of S. emarginatus Vahl. and M. pubescens J.E.Sm eachwere showed bands of different Rf values with range 0.50-0.90 and standard showed bands of 0.50 Rf values.Conclusion: It can be concluded that rutin constituents of each bark extract are presents and have effective components which can be utilized as auseful herb for alleviation of various illness and disorders.Keywords: Sapindus emarginatus Vahl., Morinda pubescens J.E.Sm. Ethanol extracts, High-performance thin layer chromatography, Rutin.