Differential effects of cyclooxygenase-2 (COX-2) inhibitors on endoplasmic reticulum (ER) stress in human coronary artery endothelial cells

2021 ◽  
pp. 106948
Author(s):  
Michael J. Haas ◽  
Firas Warda ◽  
Priyanka Bikkina ◽  
Marie Angelica Landicho ◽  
Poonam Kalidas ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Coronary Artery ◽  
Er Stress ◽  
Cyclooxygenase 2 ◽  
Human Coronary Artery ◽  
Differential Effects ◽  
Cox 2 ◽  
Cox 2 Inhibitors

Effect of anti-hyperglycemic drugs on endoplasmic reticulum (ER) stress in human coronary artery endothelial cells

2021 ◽  
pp. 174249
Author(s):  
Poonam Kapadia ◽  
Priyanka Bikkina ◽  
Angelica Marie Landicho ◽  
Shrina Parekh ◽  
Michael J. Haas ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Coronary Artery ◽  
Er Stress ◽  
Human Coronary Artery

scholarly journals SUN-LB137 Endocannabinoids Induce Endoplasmic Reticulum Stress in Hepatocytes and Human Coronary Artery Endothelial Cells

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Angela Richter ◽  
Shrina Parekh ◽  
Poonam Kirti Kalidas ◽  
Michael John Haas ◽  
Arshag D Mooradian
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Coronary Artery ◽  
Er Stress ◽  
High Glucose ◽  
Cannabinoid Receptor ◽  
Human Coronary Artery ◽  
Protein Kinase R ◽  
Obesity And Diabetes ◽  
In Cells

Abstract Obesity and diabetes are important risk factors for the development of coronary heart disease and stroke. Plasma endocannabinoid (EC) levels are inappropriately elevated in obesity and diabetes, and are hypothesized to play a causal role in central regulation of weight gain. Importantly, it was recently demonstrated that cannabinoid receptor 1 (CNR1) triggers cell stress and induces apoptosis in kidney tubule cells exposed to palmitic acid and high-glucose (HG). HepG2 and human coronary artery endothelial cells (HCAEC) were treated with tunicamycin (TM), thapsigargin (TG), high-glucose (HG), anandamide (AN), and 2-arachondonyl glycerol (2-AG), and endoplasmic reticulum (ER) stress was measured. In cells treated with TM, AM, and 2-AG and the UPR inhibitors 4-phenylbutyrate (4-PB) and taurodeoxycholic acid (TUDCA), both 4-PB and TUDCA prevented AN and 2-AG from promoting ER stress. ER stress in cells treated with AN and 2-AG, but not TM, was inhibited by the CNR1 antagonist rimonabant. Similar results were obtained with HCAEC. Furthermore, treatment with AN and 2-AG induced inositol requiring enzyme 1α and protein kinase R-like endoplasmic reticulum kinase phosphorylation but had no effect on their expression, while activating transcription factor 6 and binding immunoglobulin protein expression were also induced by AN and 2-AG in both HepG2 and HCAEC. Finally, AN and 2-AG treatment induced CNR1 expression in both cell lines. These results strongly suggest that EC’s promote ER stress and potentially induce liver and endothelial cell dysfunction.

High-throughput analysis identifying drugs that reduce oxidative and ER stress in human coronary artery endothelial cells

2020 ◽  
Vol 879 ◽  
pp. 173119 ◽  
Author(s):  
Michael J. Haas ◽  
Victoria Feng ◽  
Krista Gonzales ◽  
Luisa Onstead-Haas ◽  
Arshag D. Mooradian
Keyword(s):  
Endothelial Cells ◽  
Coronary Artery ◽  
Er Stress ◽  
High Throughput ◽  
Human Coronary Artery ◽  
High Throughput Analysis ◽  
Throughput Analysis

scholarly journals Myeloperoxidase-derived oxidants inhibit sarco/endoplasmic reticulum Ca2+-ATPase activity and perturb Ca2+ homeostasis in human coronary artery endothelial cells

2012 ◽  
Vol 52 (5) ◽  
pp. 951-961 ◽  
Author(s):  
Naomi L. Cook ◽  
Helena M. Viola ◽  
Victor S. Sharov ◽  
Livia C. Hool ◽  
Christian Schöneich ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Coronary Artery ◽  
Atpase Activity ◽  
Human Coronary Artery

Abstract #405 The Effect of Nicotine and Highdextrose on Endoplasmic Reticulum Stress in Human Coronary Artery Endothelial Cells

2018 ◽  
Vol 24 ◽  
pp. 98
Author(s):  
Krista Gonzales ◽  
Michael Haas ◽  
Kui-Tzu Feng ◽  
Priyanka Bikkina ◽  
Marie Angelica Landicho ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Coronary Artery ◽  
Endoplasmic Reticulum Stress ◽  
Human Coronary Artery

scholarly journals Activation of Cyclooxygenase-2 by ATF4 During Endoplasmic Reticulum Stress Regulates Kidney Podocyte Autophagy Induced by Lupus Nephritis

2018 ◽  
Vol 48 (2) ◽  
pp. 753-764 ◽  
Author(s):  
Juan Jin ◽  
Li Zhao ◽  
Wenli Zou ◽  
Wei Shen ◽  
Hongjuan Zhang ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Lupus Nephritis ◽  
Er Stress ◽  
Nuclear Translocation ◽  
Kidney Injury ◽  
Cyclooxygenase 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Levels ◽  
Cox 2 ◽  
Stress Pathway

Background/Aims: Autophagy plays an essential role in lupus nephritis (LN)-induced kidney injury, although the mechanism of action remains obscure. We investigated the role of cyclooxygenase-2 (COX-2) and the ATF4 endoplasmic reticulum (ER) stress pathway in LN-induced podocyte autophagy. Methods: We evaluated podocyte autophagy in a mouse model of LN. Protein levels of COX-2 and ATF4, and markers of autophagy, were evaluated by immunofluorescence and western blotting. To evaluate apoptosis, levels of PGE2 were measured by enzyme-linked immunosorbent assay. Results: LN induced kidney damage and dysfunction, which was associated with podocyte autophagy. COX-2 and the ATF4 ER stress pathway were induced by LN in cultured podocytes. Inhibition of COX-2 inhibited LN-induced autophagy in podocytes. In addition, blocking ER stress with 4-phenylbutyrate or RNAi partially counteracted COX-2 overexpression and LN-induced autophagy, suggesting that ER stress is required for LN-induced kidney autophagy. Furthermore, LN activated ATF4 and induced its nuclear translocation. Knockdown of ATF4 inhibited LN-induced COX-2 overexpression. Conclusions: Our study suggests a novel molecular mechanism by which COX2 overexpression, induced by the ATF4 ER stress pathway, contributes to LN-induced kidney autophagy and injury. These data demonstrate that COX-2 may be a potential therapeutic target against LN-induced nephropathy.

Abstract #411 High-Throughput Analysis Identifying Drugs that Reduce Oxidative and ER Stress in Human Coronary Artery Endothelial Cells

2018 ◽  
Vol 24 ◽  
pp. 101-102
Author(s):  
Priyanka Bikkina ◽  
Michael Haas ◽  
Marie Angelica Landicho ◽  
Krista Gonzales ◽  
Kui-Tzu Feng ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Coronary Artery ◽  
Er Stress ◽  
High Throughput ◽  
Human Coronary Artery ◽  
High Throughput Analysis ◽  
Throughput Analysis
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